A Novel Differential Predict Model Based on Matrix-Assisted Laser Ionization Time-of-Flight Mass Spectrometry and Serum Ferritin for Acute Graft-versus-Host Disease

Abstract

Clinical diagnosis of acute graft-versus-host disease (aGVHD) mainly depends on clinical manifestation and tissue biopsies, leading to a delayed diagnosis and treatment for aGVHD patients when the early symptom is insignificant. Our objective was to investigate the possibility of prewarning the risk of aGVHD before and after allogeneic hematopoietic stem cell transplantation (allo-HSCT) by serum protein profiling combined with serum ferritin. The difference in polypeptide expression before and after transplantation had been compared by using CLINPROT technology, and serum ferritin levels have been analyzed simultaneously. Through combining serum ferritin and MS spectral data, the diagnosis sensitivity and specificity of our model for prewarning severe aGVHD (III~IV°aGVHD) before transplant all increased to 90.0%, while after transplant, the sensitivity and specificity are 78.3% and 86.4%. Our joint prewarning model could predict the risk of aGVHD, especially severe aGVHD before and after transplant, which also provides a reliable method to the continuous monitoring of the condition of patients.

The impact of pretransplant serum ferritin on haploidentical hematopoietic stem cell transplant for acquired severe aplastic anemia in children and adolescents

“…The underlying mechanism is still unclear. Previous studies report pretransplant SF is positively associated with the risk of moderate to severe aGvHD 10,30,31 . Our finding demonstrated pretransplant SF ≥ 1000 ng/mL correlated with higher incidences of grade II‐IV aGvHD and grade III‐IV aGvHD in pediatric patients with SAA.…”

“…Previous studies report pretransplant SF is positively associated with the risk of moderate to severe aGvHD. 10,30,31 Our finding demonstrated pretransplant SF ≥ 1000 ng/mL correlated with higher incidences of grade II-IV aGvHD and grade III-IV aGvHD in pediatric patients with SAA. Transfusion dependency has been noted as a risk factor for aGvHD, and this effect was partially determined by iron overload.…”

High expression of heme oxygenase-1 in target organs may attenuate acute graft-versus-host disease through regulation of immune balance of TH17/Treg

“…Allogeneic hematopoietic stem cell transplantation (Allo-HSCT) is among the most effective therapies for a variety of hematological malignancies and autoimmune diseases (1,2). However, aGVHD is the main complication of allo-HSCT and causes significant morbidity and mortality (3,4). 30%-60% of patients which receive an allo-HSCT from an HLA-identical sibling donor subsequently develop grade II-gradeIV aGVHD (5).…”

Serum Proteomic Profiling of Lung Adenocarcinoma with Brain Metastasis Based on Matrix-Assisted Laser Ionization Time of Flight Mass Spectrometry Analysis

“…In addition, peak m/z 2213.4 Da, another peptide in our model for lung adenocarcinoma patients, is one fragment of fibrinogen alpha chain precursor. Fragments of fibrinogen alpha chain precursor at m/z 1264.6Da and 3245.6Da have been identified as diagnostic biomarker for nasopharyngeal carcinoma (25), IgA nephropathy(11) and acute graft versus host disease (aGVHD) (27).…”