A novel dissolution method for evaluation of polysaccharide based colon specific delivery systems: A suitable alternative to animal sacrifice

Effect of co-administration of probiotics with polysaccharide based colon targeted delivery systems to optimize site specific drug release

“…For a batch of 30 g, spheroids containing 40% Sulfasalazine (12 g), 40% guar gum (12 g), 18% microcrystalline cellulose PH 101 (5.4 g) and 2% talc (0.6 g) by weight ratios were prepared by extrusion and spheronization [44][45][46]. The size range was adjusted between 1 and 1.2 mm.…”

Novel biorelevant dissolution medium as a prognostic tool for polysaccharide-based colon-targeted drug delivery system

“…[ 1 ] This challenge is further intensified by the diverse strategies used in the formulation of colon-targeted delivery systems, their release being designed to be facilitated by stimuli such as pH,[ 2 3 ] pH and time,[ 2 ] presence of enzymes,[ 4 ] time,[ 6 7 8 9 ] using prodrug based,[ 7 ] pressure controlled,[ 10 ] and microbially triggered delivery systems. [ 11 12 13 14 15 16 17 18 ] The most commonly reported medium to evaluate all these types of delivery systems is the one that utilizes colon contents of rodents. [ 5 ] However, this medium has some serious limitations including lack of reproducibility, high cost, complexity of procedure, and vivisection.…”

“…[ 5 ] However, this medium has some serious limitations including lack of reproducibility, high cost, complexity of procedure, and vivisection. [ 6 15 ] Other dissolution media include the use of human fecal slurries and goat cecal contents, for which the element of reproducibility is even less.…”

“…Recently, we reported a unique, simple, inexpensive, and animal-sparing dissolution medium using probiotic culture for establishing the release pattern of polysaccharide-based colon-specific delivery system. [ 15 ] Fluid thioglycollate medium (FTM) containing probiotic (BIOMIX-I) composed of strains of Lactobacillus acidophilus, Lactobacillus rhamnosus, Bifidobacterium longum, Bifidobacterium bifidum, and Saccharomyces boulardii was used as dissolution medium. [ 15 ] The results obtained using this medium were found to be comparable with those obtained using the conventional rat cecal content medium.…”

“…[ 15 ] Fluid thioglycollate medium (FTM) containing probiotic (BIOMIX-I) composed of strains of Lactobacillus acidophilus, Lactobacillus rhamnosus, Bifidobacterium longum, Bifidobacterium bifidum, and Saccharomyces boulardii was used as dissolution medium. [ 15 ] The results obtained using this medium were found to be comparable with those obtained using the conventional rat cecal content medium. As the previous study was limited to spheroids as delivery system, the present study was conducted on diverse delivery systems, namely, mini matrix tablets, liquisolid tablets, and microspheres using different polysaccharides, i.e., guar gum, pectin, and xanthan gum to prove the wider application of developed medium.…”