A Novel Efficient Method to Identify β‐Glucuronidase Activity in Rat Small Intestine

Andlauer, Wilfried; Kolb, J.; Fürst, Peter

doi:10.1177/0148607100024005308

Cited by 14 publications

(7 citation statements)

References 19 publications

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“…As far as the present study is concerned, the findings showed that GT or KT proved remarkably efficient in the decrease of the liver and kidney dysfunction indices in surviving hypercholesterolemic rats, namely the AST, ALT, and GGT activities and the urea and creatinine levels. The nephroprotective effects KT were also attributed to organic acids (e.g., acetic and glucuronic acids) which are known to facilitate the detoxification process through conjugation with toxins, which they then solubilize and eliminate from the body (Andlauer et al, 2000). This supplement could, therefore, be considered as a potential strong candidate for future industrial application as a therapeutic agent against liver and kidney toxicities.…”

Section: Discussionmentioning

confidence: 99%

Protective effect of kombucha on rats fed a hypercholesterolemic diet is mediated by its antioxidant activity

Bellassoued

Ghrab

Makni‐Ayadi

et al. 2015

Pharmaceutical Biology

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Context: Kombucha (KT) is claimed to have various beneficial effects on human health, but there is very little scientific evidence available in the literature. Objective: The present study investigates the effects of Camellia sinensis (GT) Linn. (Theaceae) and KT, two natural drinks, on cholesterol and antioxidant status using a hypercholesterolemia rat model. Materials and methods: The present study compared the free-radical scavenging abilities and polyphenol levels of GT and KT. Wistar rats fed cholesterol-rich diets were given KT or GT (5 mL/kg body weight per day, po) for 16 weeks, then fasted overnight and sacrificed. The plasma lipid levels, thiobarbituric acid reactive substances (TBARS) and aspartate aminotransferase (AST), alanine aminotransferase (ALT), and g-glutamyl transpeptidase (GGT) serum levels, antioxidant activities of superoxide dismutase (SOD) and catalase (CAT), and creatinine and urea rats were examined. Results: KT had a phenolic compound of 955 ± 0.75 mg GAE/g) followed, by GT (788.92 ± 0.02 mg GAE/g). The free radical scavenging activity of KT was higher than GT. Compared with GT, KT induced lowered serum levels of TC, TG, VLDL-C, and LDL-C by 26, 27, 28, and 36%, respectively, and increased the serum level of high-density lipoprotein cholesterol (HDL-C). KT induced a 55% decrease of TBARS level in liver and 44% in kidney, compared with those of rats fed a cholesterol-rich diet alone. Moreover, CAT and SOD activities were reduced by 29 and 33%, respectively, in liver and 31 and 35%, respectively, in kidney, after oral administration of KT, compared with those of HCD-fed rats. Conclusion: The findings revealed that KT administration induced attractive curative effects on hypercholesterolemic, particularly in terms of liver-kidney functions in rats. Its effect on humans needs to be studied further.

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Section: Discussionmentioning

confidence: 99%

Protective effect of kombucha on rats fed a hypercholesterolemic diet is mediated by its antioxidant activity

Bellassoued

Ghrab

Makni‐Ayadi

et al. 2015

Pharmaceutical Biology

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show abstract

“…The nephroprotective effects kombucha were also attributed to organic acids (e.g. acetic and glucuronic acids) which are known to facilitate the detoxification process through conjugation with toxins, which they then solubilise and eliminate from the body [56]. …”

Section: Discussionmentioning

confidence: 99%

Hypoglycemic and antilipidemic properties of kombucha tea in alloxan-induced diabetic rats

Aloulou

Hamden

Elloumi

et al. 2012

BMC Complement Altern Med

153

113

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BackgroundDiabetes has become a serious health problem and a major risk factor associated with troublesome health complications, such as metabolism disorders and liver-kidney dysfunctions. The inadequacies associated with conventional medicines have led to a determined search for alternative natural therapeutic agents. The present study aimed to investigate and compare the hypoglycemic and antilipidemic effects of kombucha and black tea, two natural drinks commonly consumed around the world, in surviving diabetic rats.MethodsAlloxan diabetic rats were orally supplied with kombucha and black tea at a dose of 5 mL/kg body weight per day for 30 days, fasted overnight, and sacrificed on the 31st day of the experiment. Their bloods were collected and submitted to various biochemical measurements, including blood glucose, cholesterol, triglcerides, urea, creatinine, transaminases, transpeptidase, lipase, and amylase activities. Their pancreases were isolated and processed to measure lipase and α-amylase activities and to perform histological analysis.ResultsThe findings revealed that, compared to black tea, kombucha tea was a better inhibitor of α-amylase and lipase activities in the plasma and pancreas and a better suppressor of increased blood glucose levels. Interestingly, kombucha was noted to induce a marked delay in the absorption of LDL-cholesterol and triglycerides and a significant increase in HDL-cholesterol. Histological analyses also showed that it exerted an ameliorative action on the pancreases and efficiently protected the liver-kidney functions of diabetic rats, evidenced by significant decreases in aspartate transaminase, alanine transaminase, and gamma-glytamyl transpeptidase activities in the plasma, as well as in the creatinine and urea contents.ConclusionsThe findings revealed that kombucha tea administration induced attractive curative effects on diabetic rats, particularly in terms of liver-kidney functions. Kombucha tea can, therefore, be considered as a potential strong candidate for future application as a functional supplement for the treatment and prevention of diabetes.

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“…Andlaur et al . reported that potential phytochemical toxins were detoxified in mammalian tissue by conjugation with glucuronic acid [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of Kombucha on oxidative stress induced nephrotoxicity in rats

Gharib

2009

Chinese Medicine

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BackgroundTrichloroethylene (TCE) may induce oxidative stress which generates free radicals and alters antioxidants or oxygen-free radical scavenging enzymes.MethodsTwenty male albino rats were divided into four groups: (1) the control group treated with vehicle, (2) Kombucha (KT)-treated group, (3) TCE-treated group and (4) KT/TCE-treated group. Kidney lipid peroxidation, glutathione content, nitric oxide (NO) and total blood free radical concentrations were evaluated. Serum urea, creatinine level, gamma-glutamyl transferase (GGT) and lactate dehydrogenase (LDH) activities were also measured.ResultsTCE administration increased the malondiahyde (MDA) and NO contents in kidney, urea and creatinine concentrations in serum, total free radical level in blood and GGT and LDH activities in serum, whereas it decreased the glutathione (GSH) level in kidney homogenate. KT administration significantly improved lipid peroxidation and oxidative stress induced by TCE.ConclusionThe present study indicates that Kombucha may repair damage caused by environmental pollutants such as TCE and may be beneficial to patient suffering from renal impairment.

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A Novel Efficient Method to Identify β‐Glucuronidase Activity in Rat Small Intestine

Abstract: By using the X-GlcU-technique, it could be demonstrated convincingly that beta-glucuronidase exists in all three segments of the rat small intestine. The proposed method is an efficient, simple, and convenient method to visualize beta-glucuronidase activity.

Cited by 14 publications

References 19 publications

Protective effect of kombucha on rats fed a hypercholesterolemic diet is mediated by its antioxidant activity

Protective effect of kombucha on rats fed a hypercholesterolemic diet is mediated by its antioxidant activity

Hypoglycemic and antilipidemic properties of kombucha tea in alloxan-induced diabetic rats

Effects of Kombucha on oxidative stress induced nephrotoxicity in rats

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