A novel electrochemical biosensor based on dynamic polymerase-extending hybridization for E. coli O157:H7 DNA detection

Wang, Lijiang; Liu, Qingjun; Hu, Zhaoying; Zhang, Tracy; Wu, Chunsheng; Yang, Mo; Wang, Ping

doi:10.1016/j.talanta.2008.12.001

Cited by 101 publications

(57 citation statements)

References 23 publications

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“…Kim has prepared a capacitive humidity sensors based on anodic aluminum oxide (AAO), which has many nanosized pores, giving it a large surface area for absorbing water vapor [9]. Wang et al has reported a novel DNA biosensor based on singlestranded DNA (ssDNA) probe functionalized aluminum anodized oxide (AAO) nanopore membranes [10]. Wu et al has studied the synthesis and characterization of horseradish peroxidase (HRP) nanotubes with high density and well order using anodic aluminum oxide (AAO) as templates, in combination with EFDLA deposition technique [11].…”

Section: Introductionmentioning

confidence: 99%

Amperometric biosensor for hydrogen peroxide based on the co-electrodeposition of horseradish peroxidase and methylene blue on an ITO electrode modified with an anodic aluminum oxide template

Liu

Sun

et al. 2011

Microchim Acta

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We report on a nano-array sensor for hydrogen peroxide (H 2 O 2 ) that is based on a nanoporous anodic aluminum oxide template. This was used as a matrix for the co-immobilization of horseradish peroxidase (HRP) and methylene blue (MB) on the surface of an indium tin oxide electrode. The immobilized HRP retained its natural activity and MB is capable of efficiently shuttle electrons between HRP and the electrode. The new electrode was characterized by SEM and electrochemical methods. It exhibits fast response, long-term stability, high sensitivity and good selectivity to H 2 O 2 . Under optimized conditions, it linearly responds to H 2 O 2 in the concentration range from 1.0 μM to 26 mM, with a detection limit of 0.21 μM (at S/N03).

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Section: Introductionmentioning

confidence: 99%

Amperometric biosensor for hydrogen peroxide based on the co-electrodeposition of horseradish peroxidase and methylene blue on an ITO electrode modified with an anodic aluminum oxide template

Liu

Sun

et al. 2011

Microchim Acta

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“…Many nanoporous membrane based electrochemical sensors have been used in various applications including ion channel detection [19], DNA hybridization sensing [20,21], virus detection [22], cell based biosensing [23] and bacteria detection [24,25]. However, the current nanoporous membrane based methods were only used for single type of bacteria detection and could not realize the simultaneous detection of multiple types of bacteria from the mixed bacteria samples.…”

Section: Concurrent Detection Of Foodborne Pathogensmentioning

confidence: 99%

Concurrent Detection of Foodborne Pathogens: Past Efforts and Recent Trends

Evrendilek¹,

Richter²

2017

J Food Microbiol Saf Hyg

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Rapid and specific identification of foodborne pathogens is important for not only for food producers but also consumers as well as food safety authorities. Foods can harbor pathogen bacteria of different species, thus it is also important to be able to detect different pathogens simultaneously from the food samples even though they are in very low number. Detection of bacteria depends on different factors such as the initial number, food matrix, sensitivity of the method, competitor microflora and physical state of the bacteria whether they are injured or not. Simultaneous detection of different bacteria yet adds another hurdle for the level of detection, and thus, different approaches have developed and tested so far to optimize the maximum recovery.

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“…that occur at the electrode-electrolyte surface [1][2][3]. As a non-destructive and convenient method, EIS has been extensively applied for various biosensors to monitor cell culture [4], antibody-antigen interaction [5,6] and DNA-DNA hybridization [7,8].…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, at the nanoscale, as the diameter of the nanopore is comparable to the size of biomolecules, it is also possible to observe the effect of the volume exclusion in conductance change. Such a blocking effect can be further amplified by nanoparticle tags attached on target molecules [6,8,14,[19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

A novel electrochemical biosensor based on dynamic polymerase-extending hybridization for E. coli O157:H7 DNA detection

Cited by 101 publications

References 23 publications

Amperometric biosensor for hydrogen peroxide based on the co-electrodeposition of horseradish peroxidase and methylene blue on an ITO electrode modified with an anodic aluminum oxide template

Amperometric biosensor for hydrogen peroxide based on the co-electrodeposition of horseradish peroxidase and methylene blue on an ITO electrode modified with an anodic aluminum oxide template

Concurrent Detection of Foodborne Pathogens: Past Efforts and Recent Trends

Impedance sensing of DNA immobilization and hybridization by microfabricated alumina nanopore membranes

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