A novel electrochemical IL-6 sensor based on Au nanoparticles-modified platinum carbon electrode

Abstract

Introduction: Interleukin-6 (IL-6) is a multifunctional polypeptide cytokine composed of two glycoprotein chains, which plays an important role in many cellular reactions, pathological processes, diagnosis and treatment of diseases and so on. The detection of IL-6 plays a promising role in the cognition of clinical diseases.Methods: 4-mercaptobenzoic acid (4-MBA) was immobilized on the gold nanoparticles modified platinum carbon (PC) electrode with the linker IL-6 antibody, and finally formed an electrochemical sensor that specifically recognized IL-6. Through the highly specific antigen-antibody reaction, the IL-6 concentration of the samples to be detected. The performance of the sensor was studied by cyclic voltammetry (CV) and differential pulse voltammetry (DPV).Results: The experimental results showed that the linear detection range of the sensor for IL-6 was 100 pg/mL–700 pg/mL and the detection limit was 3 pg/mL. In addition, the sensor had the advantages of high specificity, high sensitivity, high stability and reproducibility under the interference environment of bovine serum albumin (BSA), glutathione (GSH), glycine (Gly) and neuron specific enolase (NSE), which provided a prospect for specific antigen detection sensor.Discussion: The prepared electrochemical sensor successfully detected the content of IL-6 in standard and biological samples, showing excellent detection performance. No significant difference was found between the detection results of the sensor and that of ELISA. The sensor showed a very broad prospect in the application and detection of clinical samples.

Fabrication of Curcumin-Based Electrochemical Nanosensors for the Detection of Environmental Pollutants: 1,4-Dioxane and Hydrazine

“…In addition to this, hydrazine has also been extensively used in several industrial and agricultural applications; however, its prolonged exposure makes it neurotoxic, carcinogenic, mutagenic, hepatotoxic, and nephrotoxic to humans and also pollutes the environment [ 27 ]. It is also reported that an increase in the hydrazine level beyond its critical standards could cause adverse effects, hence its level should be monitored [ 28 , 29 , 30 ]. Until now, fluorescence, chemiluminescence, chromatographic, spectroscopic, and electrochemical approaches have been used to detect N 2 H 4 [ 31 , 32 ].…”

“…The electrochemical-sensing technique has recently gained popularity for its rapid detection of trace amounts of hazardous compounds in aqueous media. In addition to this, its excellent specificity, low cost, and quick response for detecting a wide range of analytes makes it an appealing method for analyzing the content of a sample by directly converting the response into an electrical signal [ 30 , 33 ]. Some reports suggest that by incorporating nanomaterials, the electrochemical response shows better sensitivity and limits of detection and improves their robustness.…”

Recent Progresses in Optical Biosensors for Interleukin 6 Detection

“…IL-6 is a pleiotropic cytokine polypeptide which can produce by various cells such as lymphocytes, endothelial cells, keratinocyte, neural cells, and bone cells, under stimulation by special inducers and at the site of inflammation [ 5 , 6 ]. Knowing the structure of IL-6 is important in analyzing its detection processes, as its properties such as isoelectric point (pI) and molecular mass affect its sensing function.…”