A novel embryo identification system by direct tagging of mouse embryos using silicon-based barcodes

Novo, Sergi; Barrios, Leonardo; Santaló, Josep; Gómez-Martínez, Rodrigo; Duch, Marta; Estéve, J.; Plaza, J.A.; Nogués, Carme; Ibáñez, Elena

doi:10.1093/humrep/deq309

Cited by 26 publications

(32 citation statements)

References 10 publications

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“…1). These barcodes have already been used by our group to tag mouse embryos by their microinjection into the perivitelline space (Novo et al, 2011).…”

Section: Barcode Design and Fabricationmentioning

confidence: 99%

“…The fabrication process has been previously described (Fernández-Rosas et al, 2009;Novo et al, 2011).…”

Section: Barcode Design and Fabricationmentioning

confidence: 99%

“…As a first approach, our group developed a direct embryo identification system based on the microinjection of micrometric polysilicon barcodes, which can be read under a standard inverted microscope, into the perivitelline space of mouse pronuclear embryos (Novo et al, 2011). Despite of the good results achieved with this system in terms of embryo viability and identification rates, it was observed that some barcodes remained adhered to the cells surface after embryo hatching.…”

Section: Introductionmentioning

confidence: 99%

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Direct embryo tagging and identification system by attachment of biofunctionalized polysilicon barcodes to the zona pellucida of mouse embryos

et al. 2013

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study question: Is the attachment of biofunctionalized polysilicon barcodes to the outer surface of the zona pellucida an effective approach for the direct tagging and identification of cultured embryos?summary answer: The results achieved provide a proof of concept for a direct embryo tagging system using biofunctionalized polysilicon barcodes, which could help to minimize the risk of mismatching errors (mix-ups) in human assisted reproduction technologies.what is known already: Even though the occurrence of mix-ups is rare, several cases have been reported in fertility clinics around the world. Measures to prevent the risk of mix-ups in human assisted reproduction technologies are therefore required. study design, size, duration: Mouse embryos were tagged with 10 barcodes and the effectiveness of the tagging system was tested during fresh in vitro culture (n ¼140) and after embryo cryopreservation (n ¼ 84). Finally, the full-term development of tagged embryos was evaluated (n ¼105).participants/materials, setting, methods: Mouse pronuclear embryos were individually rolled over wheat germ agglutinin-biofunctionalized polysilicon barcodes to distribute them uniformly around the ZONA PELLUCIDA surface. Embryo viability and retention of barcodes were determined during 96 h of culture. The identification of tagged embryos was performed every 24 h in an inverted microscope and without embryo manipulation to simulate an automatic reading procedure. Full-term development of the tagged embryos was assessed after their transfer to pseudo-pregnant females. To test the validity of the embryo tagging system after a cryopreservation process, tagged embryos were frozen at the 2-cell stage using a slow freezing protocol, and followed in culture for 72 h after thawing.main results and the role of chance: Neither the in vitro or in vivo development of tagged embryos was adversely affected. The tagging system also proved effective during an embryo cryopreservation process. Global identification rates higher than 96 and 92% in fresh and frozen-thawed tagged embryos, respectively, were obtained when simulating an automatic barcode reading system, although these rates could be increased to 100% by simply rotating the embryos during the reading process.limitations, reasons for caution: The direct embryo tagging developed here has exclusively been tested in mouse embryos. Its effectiveness in other species, such as the human, is currently being tested.

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“…1). These barcodes have already been used by our group to tag mouse embryos by their microinjection into the perivitelline space (Novo et al, 2011).…”

Section: Barcode Design and Fabricationmentioning

confidence: 99%

“…The fabrication process has been previously described (Fernández-Rosas et al, 2009;Novo et al, 2011).…”

Section: Barcode Design and Fabricationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Direct embryo tagging and identification system by attachment of biofunctionalized polysilicon barcodes to the zona pellucida of mouse embryos

et al. 2013

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“…1 In fact, fabrication of biological microelectromechanical systems for a wide range of applications, such as cell tracking, 2 embryo tagging 3 or drug delivery, [4][5][6] has been achieved. The application of biological microelectromechanical systems has acquired special importance due to their potential use in creating systems able to deliver a drug, in a controlled manner, to a specific target cell.…”

Section: Introductionmentioning

confidence: 99%

Enhancing microparticle internalization by nonphagocytic cells through the use of noncovalently conjugated polyethyleneimine

Patiño

Nogués²,

Ibáñez³

et al. 2012

IJN

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Development of micro-and nanotechnology for the study of living cells, especially in the field of drug delivery, has gained interest in recent years. Although several studies have reported successful results in the internalization of micro-and nanoparticles in phagocytic cells, when nonphagocytic cells are used, the low internalization efficiency represents a limitation that needs to be overcome. It has been reported that covalent surface modification of micro-and nanoparticles increases their internalization rate. However, this surface modification represents an obstacle for their use as drug-delivery carriers. For this reason, the aim of the present study was to increase the capability for microparticle internalization of HeLa cells through the use of noncovalently bound transfection reagents: polyethyleneimine (PEI) Lipofectamine™ 2000 and FuGENE 6 ® . Both confocal microscopy and flow cytometry techniques allowed us to precisely quantify the efficiency of microparticle internalization by HeLa cells, yielding similar results. In addition, intracellular location of microparticles was analyzed through transmission electron microscopy and confocal microscopy procedures. Our results showed that free PEI at a concentration of 0.05 mM significantly increased microparticle uptake by cells, with a low cytotoxic effect. As determined by transmission electron and confocal microscopy analyses, microparticles were engulfed by plasma-membrane projections during internalization, and 24 hours later they were trapped in a lysosomal compartment. These results show the potential use of noncovalently conjugated PEI in microparticle internalization assays.

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“…Therefore, a careful approach is required, because no studies with sufficiently long durations have been performed to ensure that there are no effects of electromagnetic waves [15][16][17][18][19]. Recent studies have investigated direct insertions into the zona pellucida and perivitelline space in each embryo using a barcode made of polysilicon with the goal of preventing mix-ups [20][21][22][23][24]. It has been reported that polysilicon barcode tagging can be used to identify individual mouse embryos.…”

Section: Discussionmentioning

confidence: 99%

Development of a security system for assisted reproductive technology (ART)

Hur

Ryu

Park

et al. 2014

J Assist Reprod Genet

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Purpose In the field of assisted reproductive technology (ART), medical accidents can result in serious legal and social consequences. This study was conducted to develop a security system (called IVF-guardian; IG) that could prevent mismatching or mix-ups in ART. Materials and methods A software program was developed in collaboration with outside computer programmers. A quick response (QR) code was used to identify the patients, gametes and embryos in a format that was printed on a label. There was a possibility that embryo development could be affected by volatile organic components (VOC) in the printing material and adhesive material in the label paper. Further, LED light was used as the light source to recognize the QR code. Using mouse embryos, the effects of the label paper and LED light were examined. The stability of IG was assessed when applied in clinical practice after developing the system. A total of 104 cycles formed the study group, and 82 cycles (from patients who did not want to use IG because of safety concerns and lack of confidence in the security system) to which IG was not applied comprised the control group. Results Many of the label paper samples were toxic to mouse embryo development. We selected a particular label paper (P touch label) that did not affect mouse embryo development. The LED lights were non-toxic to the development of the mouse embryos under any experimental conditions. There were no differences in the clinical pregnancy rates between the IG-applied group and the control group (40/104=38.5 % and 30/82=36.6 %, respectively). Conclusions The application of IG in clinical practice did not affect human embryo development or clinical outcomes. The use of IG reduces the misspelling of patient names. Using IG, there was a disadvantage in that each treatment step became more complicated, but the medical staff improved and became sufficiently confident in ART to offset this disadvantage. Patients who received treatment using the IG system also went through a somewhat tedious process, but there were no complaints. These patients gained further confidence in the practitioners over the course of treatment.

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A novel embryo identification system by direct tagging of mouse embryos using silicon-based barcodes

Abstract: Our results demonstrate the feasibility of a direct embryo labeling system and constitute the starting point in the development of such systems.

Cited by 26 publications

References 10 publications

Direct embryo tagging and identification system by attachment of biofunctionalized polysilicon barcodes to the zona pellucida of mouse embryos

Direct embryo tagging and identification system by attachment of biofunctionalized polysilicon barcodes to the zona pellucida of mouse embryos

Enhancing microparticle internalization by nonphagocytic cells through the use of noncovalently conjugated polyethyleneimine

Development of a security system for assisted reproductive technology (ART)

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