A novel endo-β-1,6-glucanase from the mushroom Coprinopsis cinerea and its application in studying of cross-linking of β-1,6-glucan and the wall extensibility in stipe cell walls

Liu, Xiao; Wang, Rui; Bi, Jing; Kang, Liqin; Zhou, Jinhua; Duan, Baiyun; Z, Liu; Yuan, Sheng

doi:10.1016/j.ijbiomac.2020.05.244

Cited by 16 publications

(25 citation statements)

References 47 publications

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“…Zhou et al found that endo-1,3-β-glucanase (GH16), exo-1,3-β-glucanase (GH2), and 1,3-β-glucosidase (GH55) may act synergistically to completely degrade the (1→3)-β-glucan backbone of the C. cinerea cell wall during fruiting body autolysis [ 55 ]. Furthermore, β-glucosidase BGL2 (GH3), endo-1,3(4)-β-glucanase ENG16A (GH16), and endo-1,6-β-glucanase (GH30) induce C. cinerea stipe cell wall extension [ 56 , 57 , 58 , 59 ]. GH152 is a thaumatin-like protein with 1,3-β-glucanase activity and is associated with lentinan degradation and fruiting body senescence [ 60 ].…”

Section: Discussionmentioning

confidence: 99%

Novel Insights into the Mechanism Underlying High Polysaccharide Yield in Submerged Culture of Ganoderma lucidum Revealed by Transcriptome and Proteome Analyses

Wang

Zhao

et al. 2023

Microorganisms

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Polysaccharides are crucial dietary supplements and traditional pharmacological components of Ganoderma lucidum; however, the mechanisms responsible for high polysaccharide yields in G. lucidum remain unclear. Therefore, we investigated the mechanisms underlying the high yield of polysaccharides in submerged cultures of G. lucidum using transcriptomic and proteomic analyses. Several glycoside hydrolase (GH) genes and proteins, which are associated with the degradation of fungal cell walls, were significantly upregulated under high polysaccharide yield conditions. They mainly belonged to the GH3, GH5, GH16, GH17, GH18, GH55, GH79, GH128, GH152, and GH154 families. Additionally, the results suggested that the cell wall polysaccharide could be degraded by GHs, which is beneficial for extracting more intracellular polysaccharides from cultured mycelia. Furthermore, some of the degraded polysaccharides were released into the culture broth, which is beneficial for obtaining more extracellular polysaccharides. Our findings provide new insights into the mechanisms underlying the roles that GH family genes play to regulate high polysaccharide yields in G. lucidum.

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Section: Discussionmentioning

confidence: 99%

Novel Insights into the Mechanism Underlying High Polysaccharide Yield in Submerged Culture of Ganoderma lucidum Revealed by Transcriptome and Proteome Analyses

Wang

Zhao

et al. 2023

Microorganisms

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“…Therefore, a large number of site-specific hydrolases may be required to completely depolymerize into monomers. A large number of studies have reported that the glycosidic bond in the main chain of laminarin can be broken by exo-β-1,3-glucanase/laminarinase hydrolase to release glucose monomers. − β-1,6-Glucanase releases glucose by breaking the glycosidic bond in the branched chain of laminarin. , …”

Section: Metabolic Pathways Of Algal Polysaccharidesmentioning

confidence: 99%

“…121−123 β-1,6-Glucanase releases glucose by breaking the glycosidic bond in the branched chain of laminarin. 124,125 3.2. Metabolic Pathways of Polysaccharides in Red Algae.…”

Section: Chemical Structure and Specificmentioning

confidence: 99%

Insights into Algal Polysaccharides: A Review of Their Structure, Depolymerases, and Metabolic Pathways

Liang

et al. 2022

J. Agric. Food Chem.

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In recent years, marine macroalgae with extensive biomass have attracted the attention of researchers worldwide. Furthermore, algal polysaccharides have been widely studied in the food, pharmaceutical, and cosmetic fields because of their various kinds of bioactivities. However, there are immense barriers to their application as a result of their high molecular size, poor solubility, hydrocolloid nature, and low physiological activities. Unique polysaccharides, such as laminarin, alginate, fucoidan, agar, carrageenan, porphyran, ulvan, and other complex structural polysaccharides, can be digested by marine bacteria with many carbohydrate-active enzymes (CAZymes) by breaking down the limitation of glycosidic bonds. However, structural elucidation of algal polysaccharides, metabolic pathways, and identification of potential polysaccharide hydrolases that participate in different metabolic pathways remain major obstacles restricting the efficient utilization of algal oligosaccharides. This review focuses on the structure, hydrolase families, metabolic pathways, and potential applications of seven macroalgae polysaccharides. These results will contribute to progressing our understanding of the structure of algal polysaccharides and their metabolic pathways and will be valuable for clearing the way for the compelling utilization of bioactive oligosaccharides.

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“…Despite many differences between the composition and organization of mushroom cell walls, there are some solid elements that form the core scaffold. The main saccharide components of mushroom cell walls are chitin; α-glucans; and β-glucans, mainly 1,3–1,6-β- d -glucans and others like linear 1,3-β- d -glucans and linear and branched 1,6-β- d -glucans [ 1 , 2 , 3 , 4 , 5 ]. Some of these polysaccharides, especially triple-helix 1,3–1,6-β- d -glucans, show a number of proven therapeutic properties, including immunomodulatory, anticancer and antioxidant features [ 6 , 7 , 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

“…For example, the type of glycosidic bond connecting glucose monomers, the degree of chain branching, and the length of side chains [ 2 ]. Among mushroom β-glucans, there are mainly branched glucans, where β- d -glucose molecules are connected by β-1,3 bonds and have short and numerous side chains with β-1,6-link coming off the β-1,3-backbone [ 2 , 8 , 12 , 13 , 14 ]. Mushrooms 1,3–1,6-β- d -glucans in literature are often shortly labeled as β-glucans [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Polish Wild Mushrooms as Beta-Glucan Sources

Mirończuk-Chodakowska

Witkowska

2020

IJERPH

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Mushroom beta-glucans show immunomodulatory, anticancer and antioxidant features. Numerous papers have been published in the last years on fungal polysaccharides, especially beta-glucans, demonstrating their various biological activities. However substantial data about beta-glucan contents in many mushroom species, especially wild mushrooms, are still missing. Therefore, the main objective of the study was to evaluate β-glucans in 18 species of wild mushrooms and three species of commercial mushrooms for comparison purposes. The contents of β-glucans were determined by the Megazyme method and with the Congo red method, which differ in analytical procedure. Among wild mushrooms, the highest mean β-glucan content assessed with the Megazyme method was found in Tricholoma portentosum (34.97 g/100 g DM), whereas with the Congo red method in Lactarius deliciosus (17.11 g/100 g DM) and Suillus grevillei (16.97 g/100 g DM). The β-glucans in wild mushrooms assessed with the Megazyme method were comparable to commercial mushrooms, whereas β-glucans assessed with the Congo red method were generally higher in wild mushrooms, especially in Russula vinosa, L. deliciosus and S. grevillei. This study indicates wild mushrooms as interesting material for β-glucan extraction for food industry and medicinal purposes.

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A novel endo-β-1,6-glucanase from the mushroom Coprinopsis cinerea and its application in studying of cross-linking of β-1,6-glucan and the wall extensibility in stipe cell walls

Cited by 16 publications

References 47 publications

Novel Insights into the Mechanism Underlying High Polysaccharide Yield in Submerged Culture of Ganoderma lucidum Revealed by Transcriptome and Proteome Analyses

Novel Insights into the Mechanism Underlying High Polysaccharide Yield in Submerged Culture of Ganoderma lucidum Revealed by Transcriptome and Proteome Analyses

Insights into Algal Polysaccharides: A Review of Their Structure, Depolymerases, and Metabolic Pathways

Evaluation of Polish Wild Mushrooms as Beta-Glucan Sources

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