A Novel, Enzyme-Based Method for the Wound-Surface Removal and Decontamination of Organophosphorus Nerve Agents

Grimsley, Janet K.; Singh, Waheguru Pal; Wild, James R.; Giletto, Anthony

doi:10.1021/bk-2001-0792.ch003

Cited by 14 publications

(10 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The potential utility of immobilized enzymes on cotton fabrics as an alternative to other antimicrobial textile or nonwoven surfaces is that it offers a highly selective and environmentally acceptable material. It is noteworthy that although previous reports (Grimsley et al 2001) on prolonging the stability of immobilized enzymes suggest enzyme immobilized fabrics are amenable to stability improvement, stability issues with enzymes including potential loss of activity restrict their current applications to non-reusable materials, such as biosensors and biomedical fabrics including decontamination or antimicrobial wipes.…”

Section: Introductionmentioning

confidence: 99%

Covalent attachment of lysozyme to cotton/cellulose materials: protein verses solid support activation

et al. 2011

View full text Add to dashboard Cite

Covalent attachment of enzymes to cellulosic materials like cotton is of interest where either release or loss of enzyme activity over time needs to be avoided. The covalent attachment of an enzyme to a cellulosic substrate requires either activation of a protein side chain or an organic functional group on the cellulosic substrate. Use of a water soluble carbodiimide to create an amide linkage as the covalent attachment between the enzyme and substrate represents an aqueous-based alternative which may be preferred for textile processes. Here we describe an amide bond-mediated lysozyme immobilization applied to cotton where either the carboxylate side chains of the protein or pendant carboxylates in a citrate, cross-linked cotton support are activated as the O-acyl-isourea intermediate, and the reactive amino nucleophiles are derived from amino-silanized cotton and the protein's amino side chains, respectively. A comparison is made of the two activation approaches to covalently link lysozyme to two different cotton fabrics using the water soluble carbodiimide 1-cyclohexyl-3-(2-morpholinoethyl)-carbodiimide-metho-ptoluene sulfonate. A comparison of the resulting enzyme activities of lysozyme on two different cotton supports showed that linking lysozyme to citrate crosslinked cotton gave higher activity than on aminosilanized cotton. The lysozyme-cellulose conjugate formed on the citrate crosslinked nonwoven cotton fabric gave the highest yield and antimicrobial activity.

show abstract

Section: Introductionmentioning

confidence: 99%

Covalent attachment of lysozyme to cotton/cellulose materials: protein verses solid support activation

et al. 2011

View full text Add to dashboard Cite

show abstract

“…More and more attention is paid to the possibility of using enzymes for human against CWAs. Enzymes can also be used as an antidote in the case of human exposure to CWAs (Amitai et al 2003;Cheng et al 1999;Grimsley et al 2001). Research centers conducted studies to integrate enzymes with fabric structure, from which protective clothes against the CWAs will be built (Singh et al 2004).…”

Section: Enzymes Used To Neutralize Chemical Warfare Agentsmentioning

confidence: 99%

Thermal and catalytic methods used for destruction of chemical warfare agents

Nawała

Jóźwik

Popiel

2019

Int. J. Environ. Sci. Technol.

View full text Add to dashboard Cite

The decontamination of chemical warfare agents (CWAs) from structures, environmental media and even personnel has become an area of particular interest in recent years due to increased homeland security concerns. This article reviews applications of catalytic methods used for decontamination of CWAs. Most attention was given to the following methods: thermocatalysis, photocatalysis and enzyme catalysis among the many methods of catalytic CWA decomposition. Unfortunately, there are not enough data obtained with real CWAs due to the difficulty in handling, so we have described also data available for CWAs simulants. These methods can be useful for decontamination systems that can reduce the damage caused by possible terrorism.

show abstract

“…Grimsley et al also investigated the use of cellulose for the covalent immobilization of OPH using glutaraldehyde (121). Cotton is a highly absorptive material, and in combination with nerve agent-degrading enzymes, would be appropriate for use as decontaminating towelettes, gauze, swabs, bandages, and wound dressings (121).…”

Section: Biomaterials Against Chemical Weaponsmentioning

confidence: 99%

“…Cotton is a highly absorptive material, and in combination with nerve agent-degrading enzymes, would be appropriate for use as decontaminating towelettes, gauze, swabs, bandages, and wound dressings (121). A cotton towelette was reported to degrade 0.12 mg paraoxon/min/cm 2 of fabric and should be able to degrade gram-size quantities of nerve agent in a few minutes (121). However, long-term stability may be a concern because a dry fabric with trehalose added as a stabilizer lost nearly 30% of its initial activity after 35 days (121).…”

Section: Biomaterials Against Chemical Weaponsmentioning

confidence: 99%

“…A cotton towelette was reported to degrade 0.12 mg paraoxon/min/cm 2 of fabric and should be able to degrade gram-size quantities of nerve agent in a few minutes (121). However, long-term stability may be a concern because a dry fabric with trehalose added as a stabilizer lost nearly 30% of its initial activity after 35 days (121).…”

Section: Biomaterials Against Chemical Weaponsmentioning

confidence: 99%

See 1 more Smart Citation

Biomaterials for Mediation of Chemical and Biological Warfare Agents

Russell

Berberich²,

Drevon³

et al. 2003

Annu. Rev. Biomed. Eng.

187

View full text Add to dashboard Cite

Recent events have emphasized the threat from chemical and biological warfare agents. Within the efforts to counter this threat, the biocatalytic destruction and sensing of chemical and biological weapons has become an important area of focus. The specificity and high catalytic rates of biological catalysts make them appropriate for decommissioning nerve agent stockpiles, counteracting nerve agent attacks, and remediation of organophosphate spills. A number of materials have been prepared containing enzymes for the destruction of and protection against organophosphate nerve agents and biological warfare agents. This review discusses the major chemical and biological warfare agents, decontamination methods, and biomaterials that have potential for the preparation of decontamination wipes, gas filters, column packings, protective wear, and self-decontaminating paints and coatings.

show abstract

A Novel, Enzyme-Based Method for the Wound-Surface Removal and Decontamination of Organophosphorus Nerve Agents

Cited by 14 publications

References 0 publications

Covalent attachment of lysozyme to cotton/cellulose materials: protein verses solid support activation

Covalent attachment of lysozyme to cotton/cellulose materials: protein verses solid support activation

Thermal and catalytic methods used for destruction of chemical warfare agents

Biomaterials for Mediation of Chemical and Biological Warfare Agents

Contact Info

Product

Resources

About