A Novel Enzyme-Linked Immunosorbent Assay Using the RecombinantActinobacillus pleuropneumoniaeApxII Antigen for Diagnosis of Pleuropneumonia in Pig Herds

Abstract

For the surveillance of pig herds infected with porcine pleuropneumonia, an enzyme-linked immunosorbent assay (ELISA) using the recombinant Actinobacillus pleuropneumoniae ApxII protein as species- but not serotype-specific antigen was developed. Using this ELISA, 243 of 400 animals from 22 A. pleuropneumoniae-infected herds were classified as seropositive.

Both transferrin binding proteins are virulence factors in Actinobacillus pleuropneumoniae serotype 7 infection

“…Also, it could be argued that the avirulence of this mutant is due to a synergistic e¡ect between the tbpB and ureC deletions. However, this does not seem probable, as virulence seems to be largely una¡ected by the absence of a The solid phase antigen (outer membrane and periplasma) was prepared by detergent wash as described previously [25], and the number given is reciprocal to the arithmetic means of the highest serum dilutions resulting in an optical density twice as high as the negative control serum at a dilution of 1:10. b A recombinant ApxIIA protein was used as solid phase antigen and the number given is the arithmetic mean of the serum activity (in ELISA units) as previously described [26]. c One animal was euthanized on day 4 post infection due to severe respiratory symptoms, a second animal died from circulatory failure following anesthesia 7 days post infection.…”

Both transferrin binding proteins are virulence factors inActinobacillus pleuropneumoniaeserotype 7 infection

“… b A recombinant ApxIIA protein was used as solid phase antigen and the number given is the arithmetic mean of the serum activity (in ELISA units) as previously described [26]. …”

Differential proteomic analysis reveals increased cathelicidin expression in porcine bronchoalveolar lavage fluid after an Actinobacillus pleuropneumoniae infection

“…A total of 48 specific-pathogen-free pigs of both sexes, approximately 7-to 9-weeks old, were obtained from closed herds with a long record of respiratory health. All animals were tested for the absence of A. pleuropneumoniae antibodies using an ApxIIA-ELISA [24]. The animals were experimentally infected with an A. pleuropneumoniae serotype 7 (32 pigs) or a serotype 2 clinical isolate (8 pigs) in an aerosol infection model Antimicrobial peptides in porcine BALF 77 [3]; eight pigs were used for a mock-infection.…”