A novel exopolysaccharide produced by Lactobacillus coryniformis NA-3 exhibits antioxidant and biofilm-inhibiting properties in vitro

Xu, Xiaoqing; Peng, Qing; Zhang, Yuwei; Tian, Dandan; Zhang, Pengbo; Ying, Huang; Ma, Lan; Qiao, Yu; Shi, Bo

doi:10.29219/fnr.v64.3744

Cited by 34 publications

(21 citation statements)

References 54 publications

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“…The screened strains were puri ed and cultured for two generations to obtain bacterial suspension, and total DNA was extracted from cell precipitate using TIANamp Bacteria DNA Kit. The 16S rDNA was ampli ed with primer pair 27F / 1492R using polymerized chain reaction (PCR) procedure [34]. Puri ed PCR products puri ed from each strain were sequenced by ABI3730-XL DNA Analyzer, and we used the Blast (http://www.ncbi.nlm.nih.gov.blast) to compare the spliced sequence le with the data in the NCBI 16S database [5].…”

Section: Methodsmentioning

confidence: 99%

“…The hydroxyl radical scavenging ability of EPS was measured according to the method described by Xu et al [34] with few modi cations. Brie y, 50 µL PBS solution (20 mM,pH 7.4), 25 µL 1,10-phenanthroline solution (12.5 mM), 25 µL FeSO 4 solution (2.5 mM), and 25 µL H 2 O 2 solution (20 mM) were added consecutively in a test tube.…”

Section: Hydroxyl Radical Scavenging Abilitymentioning

confidence: 99%

“…The superoxide radical scavenging activity of EPS was measured according to the method described by Xu et al [34]. The superoxide radical was generated in 50 µL of Tris-HCl buffer (pH 8.0, 150 mM) containing 25 µL of pyrogallol (1.50 mM, dissolved in 10 mM HCl) and 100 µL of EPS samples (1.0-10.0 mg/mL).…”

Section: Superoxide Radical Scavenging Activitymentioning

confidence: 99%

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Characterization and Biological Activity of a Novel Exopolysaccharide Produced By Pediococcus Pentosaceus SSC-12 From Silage

Yang

Tian

et al. 2021

Preprint

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Background: The exopolysaccharides (EPS) produced by lactic acid bacteria (LAB) are widely used in various fields because of their safety and various biological activities. In this study, we extracted and characterized the composition as well as antioxidant and antibacterial activities of EPS from Pediococcus pentosaceus SSC-12 isolated from the silage.Results: The LAB strain SSC-12 was screened and identified as Pediococcus pentosaceus, based upon 16S rDNA gene sequencing and Neighbor Joining (NJ) phylogenetic analysis. The analysis of the EPS production kinetics results of SSC-12 showed that the EPS production reached the maximum at 20 h of culture. High-performance anion exchange chromatography (HPAEC) analysis showed that the EPS produced by SSC-12 was a heteropolysaccharide comprising glucose (42.6 %), mannose (28.9 %), galactose (16.2 %), arabinose (9.4 %) and rhamnose (2.9 %). The EPS had good antioxidant activity, especially hydroxyl radical scavenging activity. When the concentration of the EPS produced by SSC-12 (SSC-12 EPS) was 10 mg/mL, its 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging ability, hydroxyl radical scavenging ability, superoxide scavenging ability, and reduction ability were 77.4 %, 97.5 %, 77.5 % and 1.3, respectively. At 10 mg/mL，SSC-12 EPS completely killed Staphylococcus aureus GDMCC 1.1220 and substantially inhibited the growth of Salmonella enterica subsp. enterica GDMCC 1.345; however, it had a weak inhibitory effect on Listeria monocytogenes GDMCC 1.347. Conclusions: Due to its strong antioxidant and antibacterial properties, EPS produced by LAB strain SSC-12 have potential application as a bioactive product in the feed, food, and pharmaceutical industries.

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Section: Methodsmentioning

confidence: 99%

Section: Hydroxyl Radical Scavenging Abilitymentioning

confidence: 99%

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Characterization and Biological Activity of a Novel Exopolysaccharide Produced By Pediococcus Pentosaceus SSC-12 From Silage

Yang

Tian

et al. 2021

Preprint

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“…Moreover, the EPS displayed potent inhibition of bacterial pathogens in biofilms formed by P. aeruginosa CMCC10104, E. coli O157:H7, S. Typhimurium ATCC13311, and S. aureus CMCC26003 (Liu et al, 2017). The disruptive effect of EPS produced by L. coryniformis NA-3 on pre-formed S. Typhimurium and B. cereus biofilms was greatest among all those tested and was reported to be 80 and 90%, respectively (Xu et al, 2020b). EPS formed by L. fermentum LB-69 isolated from children's feces inhibited biofilm formation by B. cereus RSKK 863.…”

Section: Anti-biofilm Activity and Potential Mechanismmentioning

confidence: 97%

“…The disruptive effect of EPS produced by L. coryniformis NA-3 on pre-formed S. Typhimurium and B. cereus biofilms was greatest among all those tested and was reported to be 80 and 90%, respectively ( Xu et al, 2020b ). EPS formed by L. fermentum LB-69 isolated from children’s feces inhibited biofilm formation by B. cereus RSKK 863.…”

Section: Antimicrobial Activities Of Native Lab-derived Epsmentioning

confidence: 99%

Exopolysaccharides as Antimicrobial Agents: Mechanism and Spectrum of Activity

et al. 2021

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Exopolysaccharides (EPSs) are metabolites synthesized and excreted by a variety of microorganisms, including lactic acid bacteria (LAB). EPS serve several biological functions such as interactions between bacteria and their environments, protection against hostile conditions including dehydration, the alleviation of the action of toxic compounds (bile salts, hydrolyzing enzymes, lysozyme, gastric, and pancreatic enzymes, metal ions, antibiotics), and stresses (changing pH, osmolarity), and evasion of the immune response and phage attack. Bacterial EPSs are considered valuable by the food, pharmaceutical, and nutraceutical industries, owing to their health-promoting benefits and rheological impacts. Numerous studies have reported the unusual antimicrobial activities of various EPS against a wide variety of pathogenic microbes (bacteria, virus, and fungi). This review aims to provide a comprehensive examination of the in vitro and in vivo antimicrobial activities of different EPSs, mainly against foodborne bacterial, fungal, and viral pathogens. The mechanism of EPS action against these pathogens as well as the methods used to measure antimicrobial activities are critically reviewed.

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