A novel extracellular calcium-dependent cysteine proteinase from Crithidia deanei

d’Avila-Levy, Claudia M.; Souza, Renan; Gomes, Rosana Canuto; Vermelho, Alane Beatriz; Branquinha, Marta H.

doi:10.1016/j.abb.2003.09.033

Cited by 30 publications

(27 citation statements)

References 31 publications

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“…Tccalpx11 mRNA was indeed 2.5 times more abundant on average in epimastigotes under NS than in epimastigotes growing in complete medium (Table). There were no major differences in the amounts of the total RNAs, suggesting that stage-specific expression is the result of differential mobilization of mRNAs to the polysome fraction, as described for other T. cruzi genes (Avila et al 2003).…”

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confidence: 84%

Trypanosoma cruzi: a stage-specific calpain-like protein is induced after various kinds of stress

Giese

Dallagiovanna

Marchini

et al. 2008

Mem. Inst. Oswaldo Cruz

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confidence: 84%

Trypanosoma cruzi: a stage-specific calpain-like protein is induced after various kinds of stress

Giese

Dallagiovanna

Marchini

et al. 2008

Mem. Inst. Oswaldo Cruz

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“…Our group became involved in the study of these proteins though the purification of a proteolytically active CALP in the culture supernatant of the insect trypanosomatid Crithidia deanei, yet its function was not addressed (d' Avila-Levy et al 2003). More recently, our group investigated the effect of calpain inhibitor III (MDL28170), a potent calpain inhibitor, on the growth of Leishmania amazonensis.…”

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confidence: 98%

Arrested growth ofTrypanosoma cruziby the calpain inhibitor MDL28170 and detection of calpain homologues in epimastigote forms

Sangenito¹,

Ennes-Vidal²,

Marinho³

et al. 2009

Parasitology

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In this paper, we aimed to explore the effects of the calpain inhibitor III (MDL28170) and to detect calpain-like molecules (CALPs) in epimastigote forms of Trypanosoma cruzi isolate Dm28c. MDL28170 at 70 microM promoted a powerful reduction in the growth rate after 48 h. The IC50 value was calculated to be 31.7 microM. This inhibitor promoted an increase in the cellular volume, but not cell lysis, resulting in a trypanostatic effect. T. cruzi CALPs presented a strong cross-reactivity with anti-Drosophila melanogaster calpain and anti-cytoskeleton-associated protein from Trypanosoma brucei antibodies, and labelling was found mainly intracellularly. Furthermore, an 80 kDa reactive protein was detected by Western blotting assays. No significant cross-reactivity was found with anti-human brain calpain antibody. The expression of CALPs was decreased in cells kept for long periods in axenic cultures in comparison to a strain recently isolated from mice, as well as in MDL28170-treated cells, the latter being paralleled by an increased expression of cruzipain. Different levels of CALPs expression were also detected in distinct phylogenetic lineages, like Y strain (lineage TcII), Dm28c (lineage TcI) [corrected] and INPA6147 strain (Z3 zymodeme). These results may contribute for the investigation of the functions of CALPs in trypanosomatids.

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“…EGTA, a calcium-chelating agent, is routinely used to establish the involvement of calcium ions in various physiological processes. [22][23][24][25][26][27][28][29][30][31][32][33][34][35] It forms stable, water-soluble complexes with calcium ions, thereby blocking their passage across plasma membrane into the cell. 24 Safadi et al 25 reported the presence of a calcium-dependent proteinase (CDP) activity in the roots of Arabidopsis, using in gel assays.…”

Section: Resultsmentioning

confidence: 99%

Co-localization of putative calcium channels (phenylalkylamine-binding sites) on oil bodies in protoplasts from dark-grown sunflower seedling cotyledons

Vandana

Bhatla

2009

Plant Signaling & Behavior

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IntroductionOilseeds store oil in the form of triacylglycerols (TAGs) in the organelles known as oil bodies. TAGs are rapidly converted to soluble metabolites via specialized metabolic pathways upon initiation of seed germination. 1 The protein constituents of oil bodies belong to the three major groups, comprising of structural proteins (oleosins and caleosins), enzymes (steroleosins and lipases) and minor proteins, like aquaporins. 2 The sequence of events prior to TAG hydrolysis by lipases to produce free fatty acids and glycerol, involves degradation of oleosins by a 65 kDa thiol protease 3,4 and phospholipid degradation by phospholipases. 5 This renders TAG matrix susceptible to lipase action. Oleosins are known to provide stability to oil bodies while caleosins play an important role in the biogenesis and degradation of oil bodies. 6,7 Calcium binding capacity of caleosins has been demonstrated in Arabidopsis and Sesamum indicum. 8,9 Recently, calcium concentrations as low as 100 nM have been shown to strongly modify the shape and aggregation state of purified oil bodies, thereby establishing the role of caleosins not only as a target for calcium-binding in artificial and purified oil bodies, but also as an effecter of oil body stability. 2 During seed germination, caleosins are involved in the interaction of lipid bodies with vacuoles and, thus, they play a role in the mobilization of storage lipids. 10 Based on these findings and observations from the author's laboratory on calcium-dependent proteolysis, the possibility of cation channels on/around the oil body surface for facilitating calcium availability could not be ruled out.In plant cells, ion channels permeable to Ca 2+ are so far known to exist on the plasma membrane, endoplasmic reticulum, tonoplast, nuclear and plastid membranes. 11 Electrophysiological studies have elucidated that plants possess Ca 2+ channels with different types of gating mechanisms, namely ligand-, voltageand stretch-activated. 12 Ca 2+ channels in plant cells are similar to the L-type channels characterized in animal systems. 13

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A novel extracellular calcium-dependent cysteine proteinase from Crithidia deanei

Cited by 30 publications

References 31 publications

Trypanosoma cruzi: a stage-specific calpain-like protein is induced after various kinds of stress

Trypanosoma cruzi: a stage-specific calpain-like protein is induced after various kinds of stress

Arrested growth ofTrypanosoma cruziby the calpain inhibitor MDL28170 and detection of calpain homologues in epimastigote forms

Co-localization of putative calcium channels (phenylalkylamine-binding sites) on oil bodies in protoplasts from dark-grown sunflower seedling cotyledons

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