A novel extracellular peroxidase and nucleases from a milky sap of Chelidonium majus

Nawrot, Robert; Leśniewicz, Krzysztof; Pieńkowska, Joanna R.; Goździcka-Józefiak, Anna

doi:10.1016/j.fitote.2007.04.012

Cited by 26 publications

(25 citation statements)

References 27 publications

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“…Lately using affinity purification on HT Heparin column (GE Healthcare), one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (1-D SDS-PAGE) and liquid chromatography-tandem mass spectrometry analysis (LC-ESI-MS/MS), we have identified a novel extracellular peroxidase (CMP) in the milky sap of Ch. majus L., which was accompanied by two nucleases (CMN1 and CMN2) (Nawrot et al, 2007). This finding supports the assumption, that the biological activity and curing properties of this plant's sap may be also connected with the presence of proteins.…”

Section: Introductionsupporting

confidence: 85%

Proteomic analysis of Chelidonium majus milky sap using two-dimensional gel electrophoresis and tandem mass spectrometry

2007

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Section: Introductionsupporting

confidence: 85%

Proteomic analysis of Chelidonium majus milky sap using two-dimensional gel electrophoresis and tandem mass spectrometry

2007

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“…Although previous studies have focused on the antibacterial activities of the fruit extracts of this plant (ACHI, 2006;AWOFISAYO et al, 2010), this study indicated the antibacterial activity of its stem bark extract against human bacterial pathogen. The antibacterial activity of the ethanol extract may be attributed to the presence of many pharmacologically bioactive compounds such as alkaloids, flavonoids, tannins, anthraquinones and phenolic compounds which have been previously associated with the antibacterial activity of many plants (EDEOGA et al, 2005;NAWROT et al, 2007). To have attained such degree of antibacterial activity, the extract may have possessed certain membrane active compounds capable of disrupting the function and permeability of biological membranes (ABEL et al, 2002;RAMACHANDRAN et al, 2004).…”

Section: Resultsmentioning

confidence: 99%

In vitro inhibitory effects of the ethanol extract of Tetrapleura tetraptera (Schum and thonn.) taub. against multidrug resistant staphylococcus aureus

Olajuyigbe¹,

Afolayan²

2018

Kragujevac J Science

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ABSTRACT. In this study, the antibacterial effect of ethanol extract of Tetrapleura tetraptera was investigated in vitro against methicillin-resistant Staphylococcus aureus by agar diffusion and macrobroth dilution methods. At the lowest concentration of 20 mg/ml of the ethanol extract, 100 µl produced inhibition zones that ranged between 06 and 15 ± 1.0 mm while the inhibition zones ranged between 16 ± 1.0 mm and 22 ± 1.0 mm when the isolates were tested with 100 µl of the highest concentration (100 mg/ml) of ethanol extract. The minimum inhibitory concentrations (MICs) of the ethanol extract were between 0.019 mg/ml and 5.0 mg/ml while its minimum bactericidal concentrations (MBCs) ranged between 0.078 and 10.0 mg/ml. Ten strains had their MICs less than 1.0 mg/ml while the remaining S. aureus strains had their MICs at concentrations ranging between 1.25 mg/ml and 5.0 mg/ml. The degree of antibacterial activity exhibited by the extract of T. tetraptera demonstrated that its herbal medicine could be as effective as modern medicine in treating diseases associated with the test pathogenic organism and justifying its traditional use in the treatment of bacterial infections.

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“…9), the antimicrobial compounds from the C. majus extract induced important changes at the molecular level. The C. majus extract contains a large number of alkaloids and polyphenols, and is therefore, known for its antimicrobial activity (Meng et al, 2009;Nawrot et al, 2007;Zuo et al, 2011). The main alkaloids identified in C. majus extracts are chelidonine, chelerythrine, sanguinarine, coptisine, and berberine (Sárközi et al, 2006a;Zuo et al, 2011).…”

Section: Measurement Of Induced Chlorophyll Fluorescence Parametersmentioning

confidence: 99%

Chemical Composition of Celandine (<i>Chelidonium majus</i> L.) Extract and its Effects on <i>Botrytis tulipae</i> (Lib.) Lind Fungus and the Tulip

Pârvu

Vlase

Fodorpataki

et al. 2013

Not Bot Hort Agrobot Cluj

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In this study, the content of chelidonine and berberine alkaloids, and sterols and phenols in the Chelidonium majus plant extract were analyzed. Subsequently, the effects of the extract on the germination and growth of Botrytis tulipae fungus on nutritive medium were compared to the effects of fluconazole. The plant extract was used at the minimum inhibitory concentration on B. tulipae developed in tulip leaves and the in vivo effects were investigated. The influence of different concentrations of C. majus extract on the physiological processes of the tulip (gas exchange parameters, photosynthetic light use efficiency, and induced chlorophyll fluorescence) were also tested to assess the applicability of the extract for the protection of ornamental plants against fungal infection. Our results demonstrated that 2% celandine extract does not significantly change the gas exchange parameters (transpiration rate, carbon dioxide uptake, and stomatal conductivity) of leaves exposed for 2 h, and does not interfere with the photochemical processes in the leaves. However, in higher concentrations, it increases the transpiration rate and net carbon dioxide influx. At concentrations of 15% and 20%, the extract lowers the potential quantum yield efficiency of photosystem II and the vitality index of the photosynthetic apparatus. Therefore we recommend the use of lower concentrations (≤6%) of celandine extract for the biological protection of tulips against gray mold.

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A novel extracellular peroxidase and nucleases from a milky sap of Chelidonium majus

Cited by 26 publications

References 27 publications

Proteomic analysis of Chelidonium majus milky sap using two-dimensional gel electrophoresis and tandem mass spectrometry

Proteomic analysis of Chelidonium majus milky sap using two-dimensional gel electrophoresis and tandem mass spectrometry

In vitro inhibitory effects of the ethanol extract of Tetrapleura tetraptera (Schum and thonn.) taub. against multidrug resistant staphylococcus aureus

Chemical Composition of Celandine (<i>Chelidonium majus</i> L.) Extract and its Effects on <i>Botrytis tulipae</i> (Lib.) Lind Fungus and the Tulip

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