“…First the defecation frequency of unc-10(e102) mutants (kind gift from Prof. Joohong Ahan, Hanyang University, Korea) was compared with that of the wild type N2. As shown in Figure 1 (A), time intervals between defecation in mutant worms were slightly longer than the wild type, to a degree similar to that of the N2 worms with CFL-1 RNAi knock-down (Kim et al 2012 ). Figure 1.…”
Section: Resultssupporting
confidence: 56%
“…In the previous study, restricted expression of GFP:: cfl-1 promoter fusion reporter at the anus and chemosensory amphid neurons has led us to investigate if CFL-1 is involved in functions associated with these areas. Indeed, we observed changes in the defecation frequency and daumone response in wild type N2 worms when CFL-1 activity was down-regulated by RNAi (Kim et al 2012 ). Such results are reminiscent of the fact that FBXL20, the mammalian homolog of CFL-1, is involved in the release of synaptic vesicle at the active zone via ubiquitination of Rim1protein (Yao et al 2007 ).…”
Section: Resultsmentioning
confidence: 94%
“…As the single LRR-type F-box protein in C. elegans , CFL-1 may portray a prototype exerting functions that are allocated among multiple FBXLs in higher organisms. Further investigation on the molecular functions of CFL-1, in particular concerning the calmodulin-binding motif (Chen et al 2011 ; Kim et al 2012 ), would provide better understanding towards neuronal regulation and aging in C. elegans .…”
Section: Resultsmentioning
confidence: 99%
“…In stark contrast to such diversity, C . elegans appears to encode only one F-box protein with the authentic LRR_cc domain (Kim et al 2012 ). The solitary presence of FBXL in C .…”
Section: Introductionmentioning
confidence: 99%
“…Previously we reported that this novel C . elegans FBXL, which we named CFL-1, is highly homologous to mammalian FBXL20 (Kim et al 2012 ). Despite the evolutionary distance between nematode and mammals, the homology was extended beyond the F-box motif and LRR domain.…”
Previously we reported that CFL-1, the single LRR-type F-box protein in the Caenorhabditis elegans genome, affected defecation behavior and daumone response. CFL-1 is highly homologous to the FBXL20 in mammals, which regulates synaptic vesicle release by targeting its substrate Rim1 for ubiquitin-mediated degradation. The worm homolog of Rim1 is UNC-10, a presynaptic membrane protein that triggers synaptic vesicle fusion through interaction with RAB-3 GTPase. To examine if CFL-1 exerts its modulatory effect on the defecation and daumone response via ubiquitination of UNC-10, we performed RNAi knock-down of CFL-1 in the unc-10(e102) mutant background. We noticed additive increase in defecation interval when the activities of both CFL-1 and UNC-10 were compromised. Also, the degree of dauer formation upon daumone treatment in unc-10 mutants treated with CFL-1 RNAi decreased further than the level observed in untreated mutants or wild type N2 worms with CFL-1 RNAi knock-down. Our data suggest that CFL-1 affects defecation frequency and daumone response in C. elegans through the ubiquitination of UNC-10.
“…First the defecation frequency of unc-10(e102) mutants (kind gift from Prof. Joohong Ahan, Hanyang University, Korea) was compared with that of the wild type N2. As shown in Figure 1 (A), time intervals between defecation in mutant worms were slightly longer than the wild type, to a degree similar to that of the N2 worms with CFL-1 RNAi knock-down (Kim et al 2012 ). Figure 1.…”
Section: Resultssupporting
confidence: 56%
“…In the previous study, restricted expression of GFP:: cfl-1 promoter fusion reporter at the anus and chemosensory amphid neurons has led us to investigate if CFL-1 is involved in functions associated with these areas. Indeed, we observed changes in the defecation frequency and daumone response in wild type N2 worms when CFL-1 activity was down-regulated by RNAi (Kim et al 2012 ). Such results are reminiscent of the fact that FBXL20, the mammalian homolog of CFL-1, is involved in the release of synaptic vesicle at the active zone via ubiquitination of Rim1protein (Yao et al 2007 ).…”
Section: Resultsmentioning
confidence: 94%
“…As the single LRR-type F-box protein in C. elegans , CFL-1 may portray a prototype exerting functions that are allocated among multiple FBXLs in higher organisms. Further investigation on the molecular functions of CFL-1, in particular concerning the calmodulin-binding motif (Chen et al 2011 ; Kim et al 2012 ), would provide better understanding towards neuronal regulation and aging in C. elegans .…”
Section: Resultsmentioning
confidence: 99%
“…In stark contrast to such diversity, C . elegans appears to encode only one F-box protein with the authentic LRR_cc domain (Kim et al 2012 ). The solitary presence of FBXL in C .…”
Section: Introductionmentioning
confidence: 99%
“…Previously we reported that this novel C . elegans FBXL, which we named CFL-1, is highly homologous to mammalian FBXL20 (Kim et al 2012 ). Despite the evolutionary distance between nematode and mammals, the homology was extended beyond the F-box motif and LRR domain.…”
Previously we reported that CFL-1, the single LRR-type F-box protein in the Caenorhabditis elegans genome, affected defecation behavior and daumone response. CFL-1 is highly homologous to the FBXL20 in mammals, which regulates synaptic vesicle release by targeting its substrate Rim1 for ubiquitin-mediated degradation. The worm homolog of Rim1 is UNC-10, a presynaptic membrane protein that triggers synaptic vesicle fusion through interaction with RAB-3 GTPase. To examine if CFL-1 exerts its modulatory effect on the defecation and daumone response via ubiquitination of UNC-10, we performed RNAi knock-down of CFL-1 in the unc-10(e102) mutant background. We noticed additive increase in defecation interval when the activities of both CFL-1 and UNC-10 were compromised. Also, the degree of dauer formation upon daumone treatment in unc-10 mutants treated with CFL-1 RNAi decreased further than the level observed in untreated mutants or wild type N2 worms with CFL-1 RNAi knock-down. Our data suggest that CFL-1 affects defecation frequency and daumone response in C. elegans through the ubiquitination of UNC-10.
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