A Novel Family of Transporters Mediating the Transport of Glutathione Derivatives in Plants

Zhang, Mingyong; Bourbouloux, Andrée; Cagnac, Olivier; Srikanth, Chittur V.; Rentsch, Doris; Bachhawat, Anand K.; Delrot, Serge

doi:10.1104/pp.103.030940

Cited by 90 publications

(81 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, the glutathione uptake capacity was observed only when the yeasts were grown in a sulfur-deprived medium (SD-S) where the only source of sulfur is glutathione, confirming previous results (Bogs et al, 2003;Zhang et al, 2004). Concentration dependence studies showed that the initial rate of [ 3 H]GSH uptake (measured within the first 3 min of incubation) was not fully saturable up to 5 mM (Fig.…”

Section: Atopt6supporting

confidence: 73%

“…The Saccharomyces cerevisiae OPT1 protein was reported to transport the pentapeptides YGGFL and YGGFM (Leu-and Met-enkephalin;Hauser et al, 2000), reduced glutathione (GSH), oxidized glutathione (GSSG), and glutathione conjugates (Bourbouloux et al, 2000). Likewise, BjGT1, an OPT member from Indian mustard (Brassica juncea), and OsGT1, an OPT homolog from rice (Oryza sativa), were shown to transport GSSG and GS conjugates (Bogs et al, 2003;Zhang et al, 2004). In this respect, the OPT family resembles the last family of Arabidopsis peptide transporters, AtMRP1-4, which mediates the transport of GS conjugates (Lu et al, 1997(Lu et al, , 1998Sanchez-Fernandez et al, 1998).…”

mentioning

confidence: 99%

See 1 more Smart Citation

AtOPT6 Transports Glutathione Derivatives and Is Induced by Primisulfuron

Cagnac¹,

Bourbouloux²,

Chakrabarty³

et al. 2004

Plant Physiology

Self Cite

110

View full text Add to dashboard Cite

The oligopeptide transporter (OPT) family contains nine members in Arabidopsis. While there is some evidence that AtOPTs mediate the uptake of tetra-and pentapeptides, OPT homologs in rice (Oryza sativa; OsGT1) and Indian mustard (Brassica juncea; BjGT1) have been described as transporters of glutathione derivatives. This study investigates the possibility that two members of the AtOPT family, AtOPT6 and AtOPT7, may also transport glutathione and its conjugates. Complementation of the hgt1met1 yeast double mutant by plant homologs of the yeast glutathione transporter HGT1 (AtOPT6, AtOPT7, OsGT1, BjGT1) did not restore the growth phenotype, unlike complementation by HGT1. By contrast, complementation by AtOPT6 restored growth of the hgt1 yeast mutant on a medium containing reduced (GSH) or oxidized glutathione as the sole sulfur source and induced uptake of [ 3 H]GSH, whereas complementation by AtOPT7 did not. In these conditions, AtOPT6-dependent GSH uptake in yeast was mediated by a high affinity (K m 5 400 mM) and a low affinity (K m 5 5 mM) phase. It was strongly competed for by an excess oxidized glutathione and glutathione-N-ethylmaleimide conjugate. Growth assays of yeasts in the presence of cadmium (Cd) suggested that AtOPT6 may transport Cd and Cd/GSH conjugate. Reporter gene experiments showed that AtOPT6 is mainly expressed in dividing areas of the plant (cambium, areas of lateral root initiation). RNA blots on cell suspensions and real-time reverse transcription-PCR on Arabidopsis plants indicated that AtOPT6 expression is strongly induced by primisulfuron and, to a lesser extent, by abscisic acid but not by Cd. Altogether, the data show that the substrate specificity and the physiological functions of AtOPT members may be diverse. In addition to peptide transport, AtOPT6 is able to transport glutathione derivatives and metal complexes, and may be involved in stress resistance.As other eucaryotic cells and procaryotes, plant cells have the ability to transport peptides across membranes. Peptide transport is important for storage and mobilization of reduced nitrogen (Higgins and Payne, 1977), and it is also involved in a wide range of cellular processes, including quorum sensing in bacteria, yeast mating, and the immune response in mammals (Stacey et al., 2002b). Furthermore, peptide-derived antibiotics (Dantzig et al., 1992) and anticancer agents (Hori et al., 1993) are also transported by peptide transporters.The presence of multiple peptide transporters in the Arabidopsis genome and the known functions of peptide transport in bacteria, fungi, and animals suggest that peptide transporters may also play a key role in plant growth and development (Stacey et al., 2002a). Three gene families have been shown to transport various peptides in Arabidopsis, i.e. the peptide transporter (PTR) family, the oligopeptide transporter (OPT) family, and the multidrug resistance-associated protein (MRP) family.The Arabidopsis genome contains 51 PTR family members (Stacey et al., 2002a). AtPTR2, the best known member o...

show abstract

Section: Atopt6supporting

confidence: 73%

mentioning

confidence: 99%

AtOPT6 Transports Glutathione Derivatives and Is Induced by Primisulfuron

Cagnac¹,

Bourbouloux²,

Chakrabarty³

et al. 2004

Plant Physiology

Self Cite

110

View full text Add to dashboard Cite

show abstract

“…In this process sulfur is fixed as cysteine by the plants after a process of reduction (Saito, 2000). Cysteine is the initial material for the production of reduced glutathione, which is responsible for detoxify cells through the elimination of free radicals and reactive species that accumulate during different types of stress (Saito, 2004;Zang, 2004). According to Tohbe et al, (1998), exogenous applications of reduced glutathione induced bud breaking on buds of grapevines of Cv.…”

Section: Resultsmentioning

confidence: 99%

Organic Alternative for Breaking Dormancy in Table Grapes Grown in Hot Regions

Corrales-Maldonado¹

2010

American Journal of Agricultural and Biological Sciences

View full text Add to dashboard Cite

Problem statement:In warm-winter regions, the need for intervention of chemical means to break bud rest becomes a dominant factor for maintaining economic production of table grapes. However, the problem is more acute when farmers want to grow organic table grapes in the absence of environmentally-friendly budbreak promoters. Approach: This study aimed to evaluate the effect of a mix of naturally occurring Garlic Compounds (GC) in comparison to the conventional use of hydrogen cyanamide to promote budbreak and its effects on cluster quality in four table grape cultivars fieldgrown in hot region (Sonora Desert). Results: Four cultivars responded to GC, the vines bursting bud about 3 weeks after application. Quality of fruit from 4 cultivars treated with GC was excellent. Clusters weigh and berry sizes were larger than other treatments. Conclusion: Ability of GC to break dormancy in table grape grown in Sonora Desert has significant implications for organic table grape production in hot regions.

show abstract

“…Later, OPT orthologs were identified in higher plants (Arabidopsis OPT1-OPT9, rice GT1, and Brassica juncea GT1) by sequence similarity searches using the complete sequence of the C. albicans OPT1 gene [73], or by RT-PCR, using primers corresponding to the conserved regions [74,75]. None of the OPT members show any significant sequence similarity to known PTRs.…”

Section: Opt Familymentioning

confidence: 99%

Nitrate transporters and peptide transporters

et al. 2007

View full text Add to dashboard Cite

In higher plants, two types of nitrate transporters, NRT1 and NRT2, have been identified. In Arabidopsis, there are 53 NRT1 genes and 7 NRT2 genes. NRT2 are high-affinity nitrate transporters, while most members of the NRT1 family are low-affinity nitrate transporters. The exception is CHL1 (AtNRT1.1), which is a dual-affinity nitrate transporter, its mode of action being switched by phosphorylation and dephosphorylation of threonine 101. Two of the NRT1 genes, CHL1 and AtNRT1.2, and two of the NRT2 genes, AtNRT2.1 and AtNRT2.2, are known to be involved in nitrate uptake. In addition, AtNRT1.4 is required for petiole nitrate storage. On the other hand, some members of the NRT1 family are dipeptide transporters, called PTRs, which transport a broad spectrum of di/tripeptides. In barley, HvPTR1, expressed in the plasma membrane of scutellar epithelial cells, is involved in mobilizing peptides, produced by hydrolysis of endosperm storage protein, to the developing embryo. In higher plants, there is another family of peptide transporters, called oligopeptide transporters (OPTs), which transport tetra/pentapeptides. In addition, some OPTs transport GSH, GSSH, GSH conjugates, phytochelatins, and metals.

show abstract

A Novel Family of Transporters Mediating the Transport of Glutathione Derivatives in Plants

Cited by 90 publications

References 53 publications

AtOPT6 Transports Glutathione Derivatives and Is Induced by Primisulfuron

AtOPT6 Transports Glutathione Derivatives and Is Induced by Primisulfuron

Organic Alternative for Breaking Dormancy in Table Grapes Grown in Hot Regions

Nitrate transporters and peptide transporters

Contact Info

Product

Resources

About