A novel fluorescence-based array biosensor: Principle and application to DNA hybridization assays

Schultz, E; Galland, Rémi; Bouëtiez, D. Du; Flahaut, T.; Planat-Chrétien, Anne; Lesbre, F.; Hoang, Antoine; Volland, Hervé; Perraut, François

doi:10.1016/j.bios.2007.10.006

Cited by 18 publications

(6 citation statements)

References 27 publications

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“…A more detailed description of the instrument has been given elsewhere 12b. 25 Each fluorescent material was evaluated for the detection of DNT in dry synthetic air. A specific setup was used to generate DNT vapors and expose the film to 150 ppbv of DNT.…”

Section: Methodsmentioning

confidence: 99%

Efficient Sensing of Explosives by Using Fluorescent Nonporous Films of Oligophenyleneethynylene Derivatives Thanks to Optimal Structure Orientation and Exciton Migration

Caron

Pasquinet

Lee

et al. 2014

Chemistry A European J

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The fluorescence of thin films of a diimine-substituted phenyleneethynylene compound can be efficiently quenched by nitroaromatic vapors, which is not the case for the unsubstituted parent compound. Thin-film porosity is usually considered to be an essential factor for efficient quenching, but in the present case the origin of the quenching is completely different, as both films are nonporous and hermetic to 2,4-dinitrotoluene (DNT) molecules. The molecular organization in the two crystallized thin films offers a low level of π stacking for both compounds, but the orientation of the phenylenethynylene fluorophore differs markedly with respect to the surface of the films. For the substituted compound, the fluorophore is almost parallel to the surface, thus making it readily available to molecules of a nitroaromatic quencher. This rationale is also observed in the case of a related compound bearing methoxy side chains instead of the long octyloxy moieties. Fluorescence-lifetime experiments show that the efficient quenching process in the nonporous crystallized films of the substituted compound is due to a fast (<70 ps) diffusion of excitons from the bulk of the film toward the surface where they are quenched, thus providing evidence of antenna effects.

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Section: Methodsmentioning

confidence: 99%

Efficient Sensing of Explosives by Using Fluorescent Nonporous Films of Oligophenyleneethynylene Derivatives Thanks to Optimal Structure Orientation and Exciton Migration

Caron

Pasquinet

Lee

et al. 2014

Chemistry A European J

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“…Thus, the tripod-functionalised glass surface previously prepared was transposed to a portable biosensor. 42 At the beginning of the experiment the fluorescence signal was very strong and stable despite continuous washing with buffer (Fig. 4), confirming the stability of the oxime linkage on the solid-phase.…”

Section: Preparation Of Fret Cassettes Based On Cyanine And/or Xanthe...mentioning

confidence: 55%

“…The detection of AFB1 through the SPIT-FRI method was performed by using the thick layer previously prepared (vide supra) and a continuous-flow device (flow rate of 100 mL min -1 , thermostated at 22 • C) previously described. 42 Once the layer fixed into the device, the EIA buffer (0.1 M phosphate buffer (pH 7.4) containing 0.15 M NaCl, 0.1% BSA and 0.01% sodium azide) was pumped through the microchannels for 30 min. Then, the activation step was performed by passing the anti-AFB1 mAb solution (1.5 mM in EIA buffer) for 40 min.…”

Section: Spit-fri Experimentsmentioning

confidence: 99%

A universal and ready-to-use heterotrifunctional cross-linking reagent for facile synthetic access to sophisticated bioconjugates

et al. 2010

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We describe for the first time, the synthesis and some bioconjugation applications of an original heterotrifunctional cross-linking reagent (also named tripod) bearing three different bioorthogonal functional groups which are fully compatible amongst themselves. Contrary to the first generation tripod recently reported by us (Org. Biomol. Chem., 2008, 6, 3065), the use of an azido group instead of the nucleophile-sensitive active carbamate moiety enables us to reach the targeted chemical orthogonality without the use of temporary aminooxy- and thiol protecting groups. Thus, the preparation of sophisticated bioconjugates through the sequential derivatisation of the tripod by means of copper-mediated 1,3-dipolar cycloaddition, oxime ligation and aqueous compatible mild thiol-alkylation reactions, is significantly simpler and more convenient. The chemoselective bioconjugation protocols were optimised through the preparation of FRET cassettes based on cyanine and/or xanthene fluorescent dye pairs and subsequent anchoring to fragile biomolecules. The applicability of this universal cross-linking reagent was also illustrated by the preparation of biochips suitable for aflatoxin B1 detection through the SPIT-FRI method.

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“…Current microarray technology, however, is expensive, time consuming, and often analyzed with laboratory bench‐top fluorescent microscopy. With increased demand for personalized point‐of‐care diagnostics and portable in‐field testing devices, however, there is now a need for rapid and portable genetic analysis techniques unbound from laboratory confinement 1.…”

Section: Introductionmentioning

confidence: 99%

Optimized DNA hybridization detection on nanocolloidal particles by dielectrophoresis

Gagnon

Senapati

2010

Electrophoresis

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Oligonucleotides of varying surface coverage are functionalized onto the surface of 100 nm silica particles and the corresponding hybridization reaction with target ssDNA is studied using dielectrophoresis (DEP). The measured DEP cross-over frequency (cof) is found to be sensitive to the oligonucleotide surface conformation. Zeta potential and particle size measurements suggest that at low oligo surface concentrations, non-specific binding of oligo to the particle surface prevents efficient hybridization. At high surface coverage, steric hindrance due to the fully stretched, tightly packed oligo conformation prevents diffusion of DNA molecules to the particle surface. The optimum surface coverage exists at intermediate coverage where the particle is found to be the least electrically conductive, and hence exhibits the lowest measured cof. A simple DEP cof measurement hence allows one to determine the optimal oligo surface coverage for increased hybridization efficiency and detection sensitivity.

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A novel fluorescence-based array biosensor: Principle and application to DNA hybridization assays

Cited by 18 publications

References 27 publications

Efficient Sensing of Explosives by Using Fluorescent Nonporous Films of Oligophenyleneethynylene Derivatives Thanks to Optimal Structure Orientation and Exciton Migration

Efficient Sensing of Explosives by Using Fluorescent Nonporous Films of Oligophenyleneethynylene Derivatives Thanks to Optimal Structure Orientation and Exciton Migration

A universal and ready-to-use heterotrifunctional cross-linking reagent for facile synthetic access to sophisticated bioconjugates

Optimized DNA hybridization detection on nanocolloidal particles by dielectrophoresis

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