A novel fluorescent probe for detection of Glutathione dynamics during ROS-induced redox imbalance

Niu, Tingting; Yin, Guoxing; Yu, Ting; Gan, Yabing; Zhang, Chenye; Chen, Juanjuan; Wu, Wei; Chen, Haimin; Li, Haitao; Yin, Peng

doi:10.1016/j.aca.2020.02.059

Cited by 17 publications

(2 citation statements)

References 45 publications

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“…Moreover, 2-dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5 dihydro-furan (TCF) as an excellent electron-absorbing fluorophore has attracted significant attention due to its good photostability and super-resolution imaging [27][28][29][30]. Although a variety of TCF-based fluorescent probes have been reported for biological imaging [31][32][33][34], to our knowledge, the study of TCF-based viscosity-sensitive probes is very limited [35][36][37]. In this work, a red-emission fluorescent probe, TCF-VIS1, was designed and prepared based on the TCF skeleton for viscosity detection (Scheme 1).…”

Section: Introductionmentioning

confidence: 99%

A Red-Emission Fluorescent Probe with Large Stokes Shift for Detection of Viscosity in Living Cells and Tumor-Bearing Mice

Wang,

Yang,

Zhong

et al. 2024

Molecules

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Abnormal viscosity is closely related to the occurrence of many diseases, such as cancer. Therefore, real-time detection of changes in viscosity in living cells is of great importance. Fluorescent molecular rotors play a critical role in detecting changes in cellular viscosity. Developing red emission viscosity probes with large Stokes shifts and high sensitivity and specificity remains an urgent and important topic. Herein, a novel viscosity-sensitive fluorescent probe (TCF-VIS1) with a large stokes shift and red emission was prepared based on the 2-dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran (TCF) skeleton. Due to intramolecular rotation, the probe itself does not fluorescence at low viscosity. With the increase in viscosity, the rotation of TCF-VIS1 is limited, and its fluorescence is obviously enhanced. The probe has the advantages of simple preparation, large Stokes shift, good sensitivity and selectivity, and low cytotoxicity, which make it successfully used for viscosity detection in living cells. Moreover, TCF-VIS1 showed its potential for cancer diagnosis at the cell level and in tumor-bearing mice by detecting viscosity. Therefore, the probe is expected to enrich strategies for the detection of viscosity in biological systems and offer a potential tool for cancer diagnosis.

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Section: Introductionmentioning

confidence: 99%

A Red-Emission Fluorescent Probe with Large Stokes Shift for Detection of Viscosity in Living Cells and Tumor-Bearing Mice

Wang,

Yang,

Zhong

et al. 2024

Molecules

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“…In this MEP5-modified nanochannel, GSH could be specifically assembled into the cavity of MEP5 through the host–guest interaction and form the MEP5/GSH channel system, which increased the positive charge of the nanochannel, thus leading to the activation of GSH transportation (“ON state”). Moreover, the assembled GSH on the MEP5 nanochannel was facilely oxidized to GSSG under H 2 O 2 conditions (MEP5/GSSG channel). − Accordingly, GSSG was released from the cavity of MEP5 and eventually decreased the activity of GSH transportation (“OFF state”). Importantly, the transport behavior of GSH through the MEP5 nanochannel could be recovered through reducing GSSG to GSH with a reducing agent dithiothreitol (DTT). , Thereby, the gating of the MEP5 nanochannel for GSH transport could be precisely controllably regulated under host–guest assembly and redox reactions (Scheme ).…”

Section: Introductionmentioning

confidence: 99%

Engineering the Redox-Driven Channel for Precisely Regulating Nanoconfined Glutathione Identification and Transport

Guan

Cheng

Zeng

et al. 2021

ACS Appl. Mater. Interfaces

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Bioinspired artificial nanochannels for molecular and ionic transport have extensive applications. However, it is still a huge challenge to achieve an intelligent transport system with high selectivity/efficiency and controllability. Inspired by glutathione transport across the plasma membrane via redox regulation, we herein designed and fabricated a redox-reactive artificial nanochannel based on the host–guest chemical strategy. The nanochannel platform achieved high selectivity/efficiency for the identification and transmission of glutathione in the confined space. In addition, this nanochannel can switch between the ON and OFF states through the redox reaction. This redox-regulated system can provide a potential application for detection/binding of biological analytes and redox-controlled drug release.

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Rational design of a new near-infrared fluorophore and apply to the detection and imaging study of cysteine and thiophenol

Yang

Zhan

et al. 2021

Analytica Chimica Acta

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A novel fluorescent probe for detection of Glutathione dynamics during ROS-induced redox imbalance

Cited by 17 publications

References 45 publications

A Red-Emission Fluorescent Probe with Large Stokes Shift for Detection of Viscosity in Living Cells and Tumor-Bearing Mice

A Red-Emission Fluorescent Probe with Large Stokes Shift for Detection of Viscosity in Living Cells and Tumor-Bearing Mice

Engineering the Redox-Driven Channel for Precisely Regulating Nanoconfined Glutathione Identification and Transport

Rational design of a new near-infrared fluorophore and apply to the detection and imaging study of cysteine and thiophenol

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