A novel fluorescent “turn-on” chemosensor for nanomolar detection of Fe(III) from aqueous solution and its application in living cells imaging

Abstract

a b s t r a c tAn electronically active and spectral sensitive fluorescent "turn-on" chemosensor (BTP-1) based on the benzo-thiazolo-pyrimidine unit was designed and synthesized for the highly selective and sensitive detection of Fe 3 þ from aqueous medium. With Fe 3 þ , the sensor BTP-1 showed a remarkable fluorescence enhancement at 554 nm (λ ex ¼314 nm) due to the inhibition of photo-induced electron transfer. The sensor formed a host-guest complex in 1:1 stoichiometry with the detection limit down to 0.74 nM. Further, the sensor was successfully utilized for the qualitative and quantitative intracellular detection of Fe 3 þ in two liver cell lines i.e., HepG2 cells (human hepatocellular liver carcinoma cell line) and HL-7701 cells (human normal liver cell line) by a confocal imaging technique.

A uracil nitroso amine based colorimetric sensor for the detection of Cu2+ions from aqueous environment and its practical applications

“…Herein, as a part of our ongoing research on chemosensors, [32][33][34][35] we have developed a simple and easy to prepare colorimetric chemosensor, namely the uracil nitroso amine derivative UNA-1 (Scheme 1), which can be used for the highly selective and sensitive recognition of Cu 2+ ions. This chemosensor gives a visual color change from colorless to clear dark yellow allowing for the naked-eye detection of Cu 2+ .…”

A water-soluble carboxylic-functionalized chemosensor for detecting Al3+ in aqueous media and living cells: Experimental and theoretical studies

“…The detection limit was found to be 1.9 μM, which is still a below the WHO guideline for Al 3 þ in the drinking water. In order to further confirm that the increase of the fluorescence depended on the Al 3 þ changes (Nandre et al, 2014), the Al 3 þ -supplemented cells ( Fig. S12B and E) were treated with 100 μM of metal chelator (desferoxamine: DFO) to remove the intracellular levels of Al 3 þ .…”

A reversible rhodamine 6G-based fluorescence turn-on probe for Fe3+ in water and its application in living cell imaging

“…A number of fluorescent probes for the selective detection of Fe 3+ ion have been exploited [17][18][19][20][21][22][23][24][25] . Most early probes showed a fluorescence quenching (turn-off) response due to paramagnetic of the Fe 3+ ion, which limits their application in biological systems 26,27 .…”