A novel Fomitiporia species associated with esca on grapevine in South Africa

Cloete, Mia; Fischer, Michael; Mostert, Lizel; Halleen, F.

doi:10.1007/s11557-013-0915-5

Cited by 41 publications

(24 citation statements)

References 16 publications

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“…Additionally from the ITS phylogeny, it could be observed that strains of Fomitiporella sp. (recorded as Hymenochaetales 1, 2 and 4 at GenBank), recorded by Cloete et al (2015) and isolated from South African grapevines wood-rots associated with esca disease, did not match any known species and represent independent taxa (Suppl. material 3 : Figure S1).…”

Section: Resultsmentioning

confidence: 99%

A discussion on the genus Fomitiporella (Hymenochaetaceae, Hymenochaetales) and first record of F. americana from southern South America

Pildain¹,

Cendoya²,

Ortiz-Santana³

et al. 2018

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Fomitiporella has traditionally been delimited based on the gross morphology of the basidiomes, hyphal structure and basdiospores. Recently, phylogenetic studies supported the incorporation of an extensive number of species within the genus. Although most of its species are nested in the ‘Phellinotus clade’ (Hymenochaetaceae, Basidiomycota), genera such as Arambarria, Inocutis and Phellinotus were not included in previous analysis. To further our understanding of the genus, new sequences from 28S and ITS nuc rDNA genes were jointly analysed with a large selection of taxa in the ‘Phellinotus clade’, also with re-examination of morphological and ecological data. Results showed several lineages in what has hitherto been considered to represent Fomitiporella, indicating that the genus is paraphyletic as presently circumscribed. There is a well-supported Fomitiporella core group that includes the type species and nine other monophyletic lineages with high support, of which those representing Arambarria, Inocutis and Phellinotus are distinct from the Fomitiporella core group by macro and micromorphological traits and/or biogeographic distribution. Fomitiporellaamericana, a species described from SE USA, was found in the Patagonian forests of southern Argentina and Chile; it is the taxon responsible for the white heart-rot found on standing Austrocedruschilensis and one of the taxa decaying wooden tiles of historic churches in Chiloé Is., Chile.

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Section: Resultsmentioning

confidence: 99%

A discussion on the genus Fomitiporella (Hymenochaetaceae, Hymenochaetales) and first record of F. americana from southern South America

Pildain¹,

Cendoya²,

Ortiz-Santana³

et al. 2018

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“…Esca is caused by a complex of fungal species, among which are the Ascomycetes Phaeoacremonium minimum and Phaeomoniella chlamydospora and Basidiomycetes, such as Fomitiporia mediterranea ( Fischer, 2006 ; Surico et al, 2008 ; Cloete et al, 2014 ). Esca symptoms are thought to be due to the combined activities of phytotoxic metabolites and cell wall-degrading proteins secreted by the pathogens ( Mugnai et al, 1999 ; Andolfi et al, 2011 ).…”

Section: Introductionmentioning

confidence: 99%

Whole-Genome Resequencing and Pan-Transcriptome Reconstruction Highlight the Impact of Genomic Structural Variation on Secondary Metabolite Gene Clusters in the Grapevine Esca Pathogen Phaeoacremonium minimum

et al. 2018

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The Ascomycete fungus Phaeoacremonium minimum is one of the primary causal agents of Esca, a widespread and damaging grapevine trunk disease. Variation in virulence among Pm. minimum isolates has been reported, but the underlying genetic basis of the phenotypic variability remains unknown. The goal of this study was to characterize intraspecific genetic diversity and explore its potential impact on virulence functions associated with secondary metabolism, cellular transport, and cell wall decomposition. We generated a chromosome-scale genome assembly, using single molecule real-time sequencing, and resequenced the genomes and transcriptomes of multiple isolates to identify sequence and structural polymorphisms. Numerous insertion and deletion events were found for a total of about 1 Mbp in each isolate. Structural variation in this extremely gene dense genome frequently caused presence/absence polymorphisms of multiple adjacent genes, mostly belonging to biosynthetic clusters associated with secondary metabolism. Because of the observed intraspecific diversity in gene content due to structural variation we concluded that a transcriptome reference developed from a single isolate is insufficient to represent the virulence factor repertoire of the species. We therefore compiled a pan-transcriptome reference of Pm. minimum comprising a non-redundant set of 15,245 protein-coding sequences. Using naturally infected field samples expressing Esca symptoms, we demonstrated that mapping of meta-transcriptomics data on a multi-species reference that included the Pm. minimum pan-transcriptome allows the profiling of an expanded set of virulence factors, including variable genes associated with secondary metabolism and cellular transport.

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“…Petri disease is caused by Phaeomoniella chlamydospora and Phaeoacremonium species (Crous et al , Mostert et al ). Wood rotting basidiomycete fungi, of which Fomitiporia species are the most prevalent, cause esca (Fischer , White et al 2011a, Cloete et al ). Basidiomycetes often co‐occur with Petri disease fungi resulting in both wood rot and leaf‐stripe (also called tiger‐stripe) symptoms and hence this condition has also been referred to as esca (Surico et al , White et al 2011b).…”

Section: Introductionmentioning

confidence: 99%

Optimisation of time of application ofTrichodermabiocontrol agents for protection of grapevine pruning wounds

Mutawila

Halleen

Mostert

2016

Australian Journal of Grape and Wine Research

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Background and Aims The application of Trichoderma species on grapevine pruning wound surfaces reduces wound infection from trunk pathogens that cause grapevine decline. The aim of this study was to determine the efficacy of pruning wound treatments containing Trichoderma as influenced by the time of pruning and time of application following pruning. Methods and Results Cultivars Chenin Blanc and Cabernet Sauvignon were pruned early and late in the winter season, respectively, corresponding to the break from the winter dormancy period and the normal pruning time for these cultivars. After pruning, the wounds were treated immediately, 6, 24, 48 or 96 h after pruning with either T. atroviride or T. harzianum. Colonisation of grapevine pruning wounds by the Trichoderma spp. was dependent on the physiological state of the vines as well as the weather conditions at pruning. In dormant vines, colonisation remained high from immediate application up to 48 h after pruning. In vines at break of dormancy, colonisation was highest at 6 and 24 h after application. Natural wound infection was higher in wounds pruned in late winter compared with that in early winter. Conclusions Applying the biocontrol agent 6 h after pruning consistently resulted in a high incidence of Trichoderma spp. in both cultivars at either early or late pruning regardless of vine physiological state or the weather conditions. Significance of Study Pruning early in the season in combination with the application of Trichoderma spp. pruning‐wound agents approximately 6 h after pruning can significantly reduce wound infection by trunk pathogens.

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A novel Fomitiporia species associated with esca on grapevine in South Africa

Cited by 41 publications

References 16 publications

A discussion on the genus Fomitiporella (Hymenochaetaceae, Hymenochaetales) and first record of F. americana from southern South America

A discussion on the genus Fomitiporella (Hymenochaetaceae, Hymenochaetales) and first record of F. americana from southern South America

Whole-Genome Resequencing and Pan-Transcriptome Reconstruction Highlight the Impact of Genomic Structural Variation on Secondary Metabolite Gene Clusters in the Grapevine Esca Pathogen Phaeoacremonium minimum

Optimisation of time of application ofTrichodermabiocontrol agents for protection of grapevine pruning wounds

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