A Novel Fragment Derived from Laminin-411 Facilitates Proliferation and Differentiation of Odontoblast-Like Cells

Abstract

The aim for the present study was to evaluate the in vitro effects of iMatrix-411 in odontoblast-like cells. To that end, iMatrix-411 was coated to both nontissue culture treated- (Non-PS) and tissue culture treated-polystyrene (TCPS) multiwells. MDPC-23 cells were seeded into noncoated (control) or coated wells. Optimal coating density and cell proliferation were assessed by cell counting kit-8 (CCK-8) at day two, day three, and day five. Osteo/odontogenic differentiation was evaluated by real-time RT-PCR and alkaline phosphatase (ALP) activity at days seven and eight, respectively. Calcific deposition of cells was visualized by alizarin red staining. Data were analyzed with post hoc Tukey HSD test (p < 0.05). Optimal coating density for iMatrix-411 was 8 μg/cm2. Exposure of MDPC-23 cells to iMatrix-411 in either non-PS or TCPS significantly enhanced proliferative activity. iMatrix-411 elevated ALP activity in both types of culture plates. iMatrix-411 significantly increased the mRNA level of OCN, BSP, OPN, ALP, and DMP-1. Meanwhile, it enhanced the expression of several integrin subunits: ITGA1, ITGA5, ITGAV, ITGB1, and ITGB5. Finally, iMatrix-411 also accelerated the mineralization at day eight in Non-PS. The results indicated iMatrix-411 stimulates proliferation and favours differentiation of odontoblast-like cells.

Laminin-Modified Dental Pulp Extracellular Matrix for Dental Pulp Regeneration

“…Sp7, a zinc finger transcription factor, could coordinately modulate RUNX2 and DLX5 proteins at levels appropriate for optimal osteo/odontoblastic differentiation and function ( Barone et al, 2014 ). Based on the slight increment of DSPP and Sp7 expression, we speculate that additional mineralized microenvironments may be required in in vitro culture ( Yuasa et al, 2004 ; Tang et al, 2018 ).…”

“…DSPP, DMP-1 as well as laminin were positive in the generated odontoblastic layer–like structures verifying the generation of the odontoblastic layer ( Figure 7 ). However, laminins showed limited capacity in inducing DSPP expression in in vitro culture ( Figure 5 ; Tang et al, 2018 ), but DSPP was positive in in vivo results ( Figure 7 ). The possible explanation is that a single growth factor cannot construct a complete odontogenic microenvironment, which requires the participation of laminin, DPEM, and TDM.…”