A novel function of Aft1 in regulating ferrioxamine B uptake: Aft1 modulates Arn3 ubiquitination in <i>Saccharomyces cerevisiae</i>

Jeong, Mi Young; Kang, Chang Min; Kim, Ji Hyun; Heo, Dong Hyuk; Chang, Miwha; Baek, In Joon; Ro, Hyeong Su; Choi, Il Dong; Kim, Tae Hyoung; Yun, Cheol Won

doi:10.1042/bj20082399

Cited by 10 publications

(6 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…About 80% of the DNA damage resulting in the development of cancer is caused by ROS such as hydrogen peroxide (H 2 O 2 ), singlet oxygen ( 1 O 2 ), and hydroxyl radical (OH). Therefore, avoiding oxidative DNA damage induced by ROS is very important for cancer prevention [ 60 ].…”

Section: Resultsmentioning

confidence: 99%

Citrus limon from Tunisia: Phytochemical and Physicochemical Properties and Biological Activities

Makni

Jemai

Kriaâ

et al. 2018

BioMed Research International

View full text Add to dashboard Cite

Natural plant extracts contain a variety of phenolic compounds which are assigned various biological activities. Our work aims to make a quantitative and qualitative characterization of the Zest (ZL) and the Flesh (FL) of lemon (Citrus limon), to valorize the pharmacological uses of lemon, by evaluating in vitro activities (DPPH, free radical scavenging and reducing power). The antibacterial, antifungal, and antiproliferative activities were sought in the ability of Citrus limon extracts to protect DNA and protein. We found that the ZL contains high amounts of phenolics responsible for the important antioxidant properties of the extract. However, the FL is richer in flavonoids than the ZL. The FL extract was also found to be more effective than the ZL in protecting plasmid DNA against the strand breakage induced by hydroxyl radicals. We also concluded that the FL extract exhibited potent antibacterial activity unlike ZL. Analysis by LC/MS-MS identified 6 compounds (Caffeoyl N-Tryptophan, Hydroxycinnamoyl-Oglucoside acid, Vicenin 2, Eriocitrin, Kaempferol-3-O- rutinoside, and Quercetin-3-rutinoside). These preliminary results showed that Citrus limon has antibacterial and antioxidant activity in vitro. It would be interesting to conduct further studies to evaluate the in vivo potential in an animal model.

show abstract

Section: Resultsmentioning

confidence: 99%

Citrus limon from Tunisia: Phytochemical and Physicochemical Properties and Biological Activities

Makni

Jemai

Kriaâ

et al. 2018

BioMed Research International

View full text Add to dashboard Cite

show abstract

“…As Aft1 has never been identified in the numerous kinetochore affinity chromatography purifications , it suggests that the Aft1-kinetochore interaction is transient and potentially plays a regulatory role. Intriguingly, Aft1 has recently been shown to interact with and regulate the ubiquitination state of Arn3 (Jeong et al 2009). As ubiquitination of the inner kinetochore protein Ctf13 (Kaplan et al 1997) and the centromeric histone H3 variant Cse4 (Collins et al 2004) contributes to the regulation of kinetochore complex formation at centromeres, it is tempting to speculate that Aft1 may play a similar role at the kinetochore.…”

Section: Discussionmentioning

confidence: 99%

Functional Genomics Analysis of theSaccharomyces cerevisiaeIron Responsive Transcription Factor Aft1 Reveals Iron-Independent Functions

et al. 2010

View full text Add to dashboard Cite

The Saccharomyces cerevisiae transcription factor Aft1 is activated in iron-deficient cells to induce the expression of iron regulon genes, which coordinate the increase of iron uptake and remodel cellular metabolism to survive low-iron conditions. In addition, Aft1 has been implicated in numerous cellular processes including cell-cycle progression and chromosome stability; however, it is unclear if all cellular effects of Aft1 are mediated through iron homeostasis. To further investigate the cellular processes affected by Aft1, we identified .70 deletion mutants that are sensitive to perturbations in AFT1 levels using genome-wide synthetic lethal and synthetic dosage lethal screens. Our genetic network reveals that Aft1 affects a diverse range of cellular processes, including the RIM101 pH pathway, cell-wall stability, DNA damage, protein transport, chromosome stability, and mitochondrial function. Surprisingly, only a subset of mutants identified are sensitive to extracellular iron fluctuations or display genetic interactions with mutants of iron regulon genes AFT2 or FET3. We demonstrate that Aft1 works in parallel with the RIM101 pH pathway and the role of Aft1 in DNA damage repair is mediated by iron. In contrast, through both directed studies and microarray transcriptional profiling, we show that the role of Aft1 in chromosome maintenance and benomyl resistance is independent of its iron regulatory role, potentially through a nontranscriptional mechanism.

show abstract

“…The transcriptional activator Aft1 in S. cerevisiae responds to iron availability and regulates the expression of FET3, FTR1, and ARN; it has also been shown to interact with Arn3/Sit1 transporters [119]. This interaction influences the ubiquitination and vacuole-dependent degradation of the protein, further illustrating that the sensing mechanisms in fungi can be adjusted accordingly for efficient iron uptake [134,135]. In C. albicans, the Arn1/Sit1 transporter is used to facilitate the utilisation of xenosiderophores such as coprogen, ferrichrysin ferricrocin, ferrirubin, and triacetyl-fusarine C [93,125].…”

Section: Siderophore Uptakementioning

confidence: 99%

Iron Assimilation during Emerging Infections Caused by Opportunistic Fungi with emphasis on Mucorales and the Development of Antifungal Resistance

Stanford

Voigt

2020

Genes

View full text Add to dashboard Cite

Iron is a key transition metal required by most microorganisms and is prominently utilised in the transfer of electrons during metabolic reactions. The acquisition of iron is essential and becomes a crucial pathogenic event for opportunistic fungi. Iron is not readily available in the natural environment as it exists in its insoluble ferric form, i.e., in oxides and hydroxides. During infection, the host iron is bound to proteins such as transferrin, ferritin, and haemoglobin. As such, access to iron is one of the major hurdles that fungal pathogens must overcome in an immunocompromised host. Thus, these opportunistic fungi utilise three major iron acquisition systems to overcome this limiting factor for growth and proliferation. To date, numerous iron acquisition pathways have been fully characterised, with key components of these systems having major roles in virulence. Most recently, proteins involved in these pathways have been linked to the development of antifungal resistance. Here, we provide a detailed review of our current knowledge of iron acquisition in opportunistic fungi, and the role iron may have on the development of resistance to antifungals with emphasis on species of the fungal basal lineage order Mucorales, the causative agents of mucormycosis.

show abstract

A novel function of Aft1 in regulating ferrioxamine B uptake: Aft1 modulates Arn3 ubiquitination in Saccharomyces cerevisiae

Cited by 10 publications

References 40 publications

Citrus limon from Tunisia: Phytochemical and Physicochemical Properties and Biological Activities

Citrus limon from Tunisia: Phytochemical and Physicochemical Properties and Biological Activities

Functional Genomics Analysis of theSaccharomyces cerevisiaeIron Responsive Transcription Factor Aft1 Reveals Iron-Independent Functions

Iron Assimilation during Emerging Infections Caused by Opportunistic Fungi with emphasis on Mucorales and the Development of Antifungal Resistance

Contact Info

Product

Resources

About