A Novel Gene Alignment in <i>Dorea</i> sp. AM58-8 Produces 7-Dehydroxy-3β Bile Acids from Primary Bile Acids

Bai, Yanjie; Zhao, Tianhu; Gao, Mengyu; Zou, Yuanqiang; Lei, Xiaoguang

doi:10.1021/acs.biochem.2c00264

Cited by 9 publications

(2 citation statements)

References 30 publications

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“…While these changes have been previously reported in humans, it is possible that changes in these metabolites also occur in deer in conjunction with the change of microbiome in CWD. It was known that Eubacteriaceae ( 52 ) and Dorea ( 53 ) facilitate the conversion of primary to secondary bile acids in the gut. In the CWD feces, they are significantly correlated with DCA; thus, the decrease of DCA could be the result of their decrease.…”

Section: Discussionmentioning

confidence: 99%

Prospective fecal microbiomic biomarkers for chronic wasting disease

Didier,

Bourner,

Kleks

et al. 2024

Microbiol Spectr

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Chronic wasting disease (CWD) is a naturally occurring prion disease in cervids that has been rapidly proliferating in the United States. Here, we investigated a potential link between CWD infection and gut microbiome by analyzing 50 fecal samples obtained from CWD-positive animals of different sexes from various regions in the USA compared to 50 CWD-negative controls using high throughput sequencing of the 16S ribosomal RNA and targeted metabolomics. Our analysis reveals promising trends in the gut microbiota that could potentially be CWD-dependent, including several bacterial taxa at each rank level, as well as taxa pairs, that can differentiate between CWD-negative and CWD-positive deer. Through machine-learning, these taxa and taxa pairs at each rank level could facilitate identification of around 70% of both the CWD-negative and the CWD-positive samples. Our results provide a potential tool for diagnostics and surveillance of CWD in the wild, as well as conceptual advances in our understanding of the disease. IMPORTANCE This is a comprehensive study that tests the connection between the composition of the gut microbiome in deer in response to chronic wasting disease (CWD). We analyzed 50 fecal samples obtained from CWD-positive animals compared to 50 CWD-negative controls to identify CWD-dependent changes in the gut microbiome, matched with the analysis of fecal metabolites. Our results show promising trends suggesting that fecal microbial composition can directly correspond to CWD disease status. These results point to the microbial composition of the feces as a potential tool for diagnostics and surveillance of CWD in the wild, including non-invasive CWD detection in asymptomatic deer and deer habitats, and enable conceptual advances in our understanding of the disease.

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Section: Discussionmentioning

confidence: 99%

Prospective fecal microbiomic biomarkers for chronic wasting disease

Didier,

Bourner,

Kleks

et al. 2024

Microbiol Spectr

View full text Add to dashboard Cite

show abstract

“…While these changes have been previously reported in humans, it is possible that changes in these metabolites also occur in deer in conjunction with the change of microbiome in CWD. It was known that Eubacteriaceae ( 46 ) and Dorea ( 47 ) facilitate the conversion of primary to secondary bile acids in gut. In the CWD feces, they are significantly correlated with DCA, thus the decrease of DCA could be the results of their decrease.…”

Section: Discussionmentioning

confidence: 99%

Prospective Fecal Microbiomics Biomarkers for Chronic Wasting Disease

Didier,

Bourner,

Kleks

et al. 2023

Preprint

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Chronic wasting disease (CWD) is a naturally occurring infectious, fatal, transmissible spongiform encephalopathy of cervids that has rapidly proliferated across North America over the past five decades. CWD belongs to a broader class of prion diseases, caused by accumulation of abnormally misfolded prion proteins. Healthy animals are believed to acquire this disease primarily by oral exposure to infected animal by-products or the environment containing misfolded prions. Thus, gastrointestinal tract represents a primary route of CWD infection. Recent studies propose a direct link between the gut microbiome and the pathogenesis of other prion diseases, however a direct link between CWD infection and gut microbiome has not been well established. Here we analyzed 50 fecal samples obtained from CWD-positive animals of different sexes from various regions in the US, compared to 50 healthy controls to identify CWD-dependent changes in the gut microbiome and the corresponding composition of key metabolites of host and microbial origin. Using high throughput sequencing of 16S ribosomal RNA gene, we identified promising trends in the gut microbiota that could potentially be CWD-dependent. While, in agreement with previously published studies, geographical origin exerts strong influence on the microbial composition of the gut, using CWD as a variable reveals 64 bacterial taxa that are differentially abundant between control and CWD-positive deer in our samples and can be explored as potential markers of CWD. Our results provide a potential tool for diagnostics and surveillance of CWD in the wild, as well as conceptual advances in our understanding of the disease.ImportanceThis is a comprehensive study that tests the connection between the composition of the gut microbiome in deer in response to Chronic Wasting Disease (CWD). We analyzed 50 fecal samples obtained from CWD-positive animals compared to 50 healthy controls to identify CWD-dependent changes in the gut microbiome, matched with the analysis of fecal metabolites. Our results show promising trends suffesting that fecal microbial composition can directly correspond to CWD disease status. Furthermore, our data suggest that early stages of CWD that are outwardly asymptomatic exhibit the same changes as the later stage of the disease. These results point to microbial composition of the feces as a potential tool for diagnostics and surveillance of CWD in the wild, including non-invasive CWD detection in asymptomatic deer and deer habitats, and enable conceptual advances in our understanding of the disease.

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Emerging chemophysiological diversity of gut microbiota metabolites

Lin,

He,

et al. 2024

Trends in Pharmacological Sciences

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A Novel Gene Alignment in Dorea sp. AM58-8 Produces 7-Dehydroxy-3β Bile Acids from Primary Bile Acids

Cited by 9 publications

References 30 publications

Prospective fecal microbiomic biomarkers for chronic wasting disease

Prospective fecal microbiomic biomarkers for chronic wasting disease

Prospective Fecal Microbiomics Biomarkers for Chronic Wasting Disease

Emerging chemophysiological diversity of gut microbiota metabolites

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