A novel gene family defined by human dihydropyrimidinase and three related proteins with differential tissue distribution

Hamajima, Naoki; Matsuda, Koichi; Sakata, Shigeko Fujimoto; Tamaki, Nanaya; Sasaki, Makoto; Nonaka, Masaru

doi:10.1016/s0378-1119(96)00445-3

Cited by 202 publications

(149 citation statements)

References 20 publications

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“…Expression of Ulip6/ CRMP5 protein was analyzed by Western blotting using a rabbit polyclonal antiserum that, as shown in Figure 1 D, recognized the Ulip6/CRMP5 recombinant protein but not the other four Ulip/ CRMPs. As for the other Ulip/CRMPs (Hamajima et al, 1996;Wang and Strittmatter, 1996;Byk et al, 1998), Ulip6/CRMP5 protein was highly expressed in the embryonic brain and showed a dramatic downregulation during ontogenesis, as illustrated in the cerebellum (Fig. 1 B).…”

Section: Molecular Characterization and Tissue Distribution Of Human mentioning

confidence: 55%

“…The Unc-33-like phosphoprotein/collapsin response mediator protein (Ulip/CRMP) family consists of four homologous cytosolic phosphoproteins (Minturn et al, 1995;Byk et al, 1996;Hamajima et al, 1996;Wang and Strittmatter, 1996;Quach et al, 1997) that are highly expressed in the developing brain and have unique and as yet poorly understood molecular mechanisms of action. Ulip2/CRMP2, the most widely studied member, is reported to mediate semaphorin-3A (Sema3A)-induced growth cone collapse through a signal transduction cascade involving heterotrimeric G-proteins (Goshima et al, 1995), growth cone collapse induced by lysophosphatidic acid acting via Rhoassociated protein kinase (Arimura et al, 2000), and neuronal apoptotic death (Shirvan et al, 1999).…”

Section: Extension; Anatomical Expression; Neurodegenerative Disordersmentioning

confidence: 99%

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Isolation and Expression Pattern of Human Unc-33-Like Phosphoprotein 6/Collapsin Response Mediator Protein 5 (Ulip6/CRMP5): Coexistence with Ulip2/CRMP2 in Sema3A- Sensitive Oligodendrocytes

Ricard¹,

Rogemond²,

Charrier³

et al. 2001

J. Neurosci.

124

104

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The Unc-33-like phosphoprotein/collapsin response mediator protein (Ulip/CRMP) family consists of four homologous phosphoproteins considered crucial for brain development. Autoantibodies produced against member(s) of this family by patients with paraneoplastic neurological diseases have made it possible to clone a fifth human Ulip/CRMP and characterize its cellular and anatomical distribution in developing brain. This protein, referred to as Ulip6/CRMP5, is highly expressed during rat brain development in postmitotic neural precursors and in the fasciculi of fibers, suggesting its involvement in neuronal migration/differentiation and axonal growth. In the adult, Ulip6/ CRMP5 is still expressed in some neurons, namely in areas that retain neurogenesis and in oligodendrocytes in the midbrain, hindbrain, and spinal cord. Ulip2/CRMP2 and Ulip6/CRMP5 are coexpressed in postmitotic neural precursors at certain times during development and in oligodendrocytes in the adult. Because Ulip2/CRMP2 has been reported to mediate semaphorin-3A (Sema3A) signal in developing neurons, in studies to understand the function of Ulip6/CRMP5 and Ulip2/ CRMP2 in the adult, purified adult rat brain oligodendrocytes were cultured in a Sema3A-conditioned medium. Oligodendrocytes were found to have Sema3A binding sites and to express neuropilin-1, the major Sema3A receptor component. In the presence of Sema3A, these oligodendrocytes displayed a dramatic reduction in process extension, which was reversed by removal of Sema3A and prevented by anti-neuropilin-1, antiUlip6/CRMP5, anti-Ulip2/CRMP2 antibodies, or VEGF-165, another neuropilin-1 ligand. These results indicate the existence in the adult brain of a Sema3A signaling pathway that modulates oligodendrocyte process extension mediated by neuropilin-1, Ulip6/CRMP5, and Ulip2/CRMP2, and they open new fields of investigation of neuron/oligodendrocyte interactions in the normal and pathological brain.

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Section: Molecular Characterization and Tissue Distribution Of Human mentioning

confidence: 55%

Section: Extension; Anatomical Expression; Neurodegenerative Disordersmentioning

confidence: 99%

Isolation and Expression Pattern of Human Unc-33-Like Phosphoprotein 6/Collapsin Response Mediator Protein 5 (Ulip6/CRMP5): Coexistence with Ulip2/CRMP2 in Sema3A- Sensitive Oligodendrocytes

Ricard¹,

Rogemond²,

Charrier³

et al. 2001

J. Neurosci.

124

104

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“…The animal homologs of DRP-2 (rat CRMP-62, mouse unc-33, chick TOAD-64) are brain-specific enzymes which play a crucial role in development. [12][13][14][15] In humans, an absence of dihydropyrimidinase itself causes severe neurological impairments occurring through delayed or arrested development, and neuronal degeneration with secondary delay of myelinMolecular Psychiatry ation. 16,17 Altered levels of DRP-2 protein are thus consistent with alterations in brain development which may play a role in a wide range of neurological and psychiatric disorders.…”

Section: Discussionmentioning

confidence: 99%

Disease-specific alterations in frontal cortex brain proteins in schizophrenia, bipolar disorder, and major depressive disorder

Cd²,

Jp³

et al. 2000

Mol Psychiatry

496

272

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Severe psychiatric disorders such as schizophrenia, bipolar disorder and major depressive disorder are brain diseases of unknown origin. No biological marker has been documented at the pathological, cellular, or molecular level, suggesting that a number of complex but subtle changes underlie these illnesses. We have used proteomic technology to survey postmortem tissue to identify changes linked to the various diseases. Proteomics uses two-dimensional gel electrophoresis and mass spectrometric sequencing of proteins to allow the comparison of subsets of expressed proteins among a large number of samples. This form of analysis was combined with a multivariate statistical model to study changes in protein levels in 89 frontal cortices obtained postmortem from individuals with schizophrenia, bipolar disorder, major depressive disorder, and non-psychiatric controls. We identified eight protein species that display disease-specific alterations in level in the frontal cortex. Six show decreases compared with the non-psychiatric controls for one or more diseases. Four of these are forms of glial fibrillary acidic protein (GFAP), one is dihydropyrimidinase-related protein 2, and the sixth is ubiquinone cytochrome c reductase core protein 1. Two spots, carbonic anhydrase 1 and fructose biphosphate aldolase C, show increase in one or more diseases compared to controls. Proteomic analysis may identify novel pathogenic mechanisms of human neuropsychiatric diseases. Molecular Psychiatry (2000) 5, 142-149.

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“…Most notably, DRP-2 and -3, members of collapsing response mediator proteins (CRMP) involved in regulation of neurite guidance and synapse formation, are expressed mainly in fetal and neonatal brains (Hamajima et al, 1996;Kitamura et al, 1999;Fountoulakis et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Identification of differentially expressed proteins in human glioblastoma cell lines and tumors

Zhang

Tremblay

McDermid

et al. 2003

Glia

112

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KEY WORDSproteomics; matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF); liquid chromatography tandem mass spectrometry (LC-MS-MS); glioblastoma; U87MG; major vault protein; cystatin B; Hsp27; tissue transglutaminase; epidermal growth factor receptor ABSTRACT An in-frame deletion of 801 bp in exons 2-7 (type III mutation) of the epidermal growth factor receptor (EGFR) is detected at high incidence in primary glioblastoma tumors. A proteomic approach was used to generate differential protein expression maps of fetal human astrocytes (FHA), human glioblastoma cell lines U87MG and U87MG expressing type III EGFR deletion (U87MG⌬EGFR) that confers high malignancy to tumor cells. Two-dimensional gel electrophoresis followed by in-gel digestion of separated spots and protein identification by LC-MS-MS and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) identified 23 proteins expressed at higher levels or exclusively in FHA and 29 proteins expressed at higher levels or exclusively in U87MG cells. Three proteins, ubiquitin, cystatin B, and tissue transglutaminase (TTG), were upregulated in U87MG⌬EGFR relative to U87MG. Four proteins highly expressed by U87MG cells, Hsp27, major vault protein, TTG, and cystatin B, were analyzed by Western blot, ELISA, or RT-PCR in cell extracts and in tissue samples of glioblastoma multiforme (GBM; grade IV), low-grade astrocytomas (grades I and II), and nonmalignant brain lesions. All four proteins were highly expressed in GBM tissues compared to nonmalignant brain. These proteins may be used as diagnostic or functional (e.g., multiple drug resistance, invasiveness) markers for glioblastoma tumors. GLIA 42:194 -208, 2003.

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A novel gene family defined by human dihydropyrimidinase and three related proteins with differential tissue distribution

Cited by 202 publications

References 20 publications

Isolation and Expression Pattern of Human Unc-33-Like Phosphoprotein 6/Collapsin Response Mediator Protein 5 (Ulip6/CRMP5): Coexistence with Ulip2/CRMP2 in Sema3A- Sensitive Oligodendrocytes

Isolation and Expression Pattern of Human Unc-33-Like Phosphoprotein 6/Collapsin Response Mediator Protein 5 (Ulip6/CRMP5): Coexistence with Ulip2/CRMP2 in Sema3A- Sensitive Oligodendrocytes

Disease-specific alterations in frontal cortex brain proteins in schizophrenia, bipolar disorder, and major depressive disorder

Identification of differentially expressed proteins in human glioblastoma cell lines and tumors

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