A Novel Genotype of Avian Infectious Bronchitis Virus Isolated in Japan in 2009

Mase, Masaji; Kawanishi, Nahoko; Ootani, Yoshiko; Murayama, Kazunori; Karino, Ayako; Inoue, Toshikazu; Kawakami, Junko

doi:10.1292/jvms.10-0080

Cited by 33 publications

(33 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In 2007, an IBV strain (designated as TC07-2) that represents a genetically distinct lineage was isolated from broilers with respiratory illness in Guangdong Province, China (Li et al, 2010). Two years later, TC07-2-like viruses were also isolated in Japan and Korea, and designated as JP-IV (Mase et al, 2010) and new cluster 2 (Lim et al, 2012) genotypes, respectively. Later, TC07-2-like viruses were designated as GVI-1 by phylogenetic analysis using complete nucleotide sequences of the S1 gene from 13 IBV isolates in China and Korea between 2007 and 2012 (Valastro et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity of avian infectious bronchitis virus in China in recent years

Han

Jiang

et al. 2018

Infection, Genetics and Evolution

View full text Add to dashboard Cite

In this study, 213 infectious bronchitis viruses (IBVs) were isolated from samples collected from 801 flocks suspected to be infected with IBV from January 2016 to December 2017 in China. By using complete nucleotide sequences of S1 gene we determined the phylogeny of these IBV isolates, which in turn allowed us to define six lineages/genotypes, a number of recombinants and a novel variant. The GI-19 lineage was the most frequently isolated type in China in recent years. Although scattered mutations in the S1 gene among the GI-19 lineage viruses were observed, we also noted different sublineages in the GI-19 lineage with unique mutations, suggesting a high degree of S1 gene variation since they were first isolated in the mid-1990s. We also isolated a number of vaccine-like viruses from vaccinated diseased chickens, although more work is needed to differentiate the reisolation of vaccine strains from field strains of the same serotype. One of the important findings in this study is that the prevalence of the TW I type viruses in GI-7 lineage has been increasing in recent years in China. Another important finding is that recombination events occurred between the predominant GI-19 lineage and the commonly used 4/91 vaccine, which gave rise to distinct IBV isolates. In addition, a novel IBV isolate, together with a reference strain in GenBank database, were found to form a novel lineage/genotype that was remarkably distinct from established lineages. The characteristics of the antigenicity, tissue tropism, pathogenicity and complete genome were required for further investigation for the recombinants and the viruses in different sublineages and novel lineages. Meanwhile, permanent monitoring of circulating strains was needed to monitor the emerging viruses and rationally modify vaccination strategies in the field situation.

show abstract

Section: Discussionmentioning

confidence: 99%

Genetic diversity of avian infectious bronchitis virus in China in recent years

Han

Jiang

et al. 2018

Infection, Genetics and Evolution

View full text Add to dashboard Cite

show abstract

“…There are 2 hypervariable regions (HVRs) at nucleotide positions 168-207 and 351-369 located within the S1 gene of IBV. 21 For the multiplex RT-PCR, the rC2U primer was designed to target the conserved sequences of the IBV H120 vaccine, IBV TW-I, and IBV TW-II strains, which are outside the HVR. 3,24 On the other hand, the differential primers for the IBV H120 vaccine, IBV TW-I, and IBV TW-II strains target positions located within the HVR, a region that is prone to genetic mutations.…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, the limited numbers of nucleotide polymorphisms and nucleotide deletions similar to wild-type strains in Taiwan were also found in the wild-type IBV strains in Japan and China after multiple sequence alignments. 19,21 Since 2010, a novel approach, multiplex amplification refractory mutation system polymerase chain reaction (ARMS PCR), which incorporates specific ending 3′-nucleotides in primers, has been used for the genotyping of wild-type and vaccine strains of virus. 4,23 Therefore, the aim of the current study was to develop 2 systems, namely ARMS RT-PCR and ARMS real-time RT-PCR (ARMS qRT-PCR) combined with HRMA, for the detection and differential diagnosis of IBV.…”

Section: Introductionmentioning

confidence: 99%

The genotyping of Infectious bronchitis virus in Taiwan by a multiplex amplification refractory mutation system reverse transcription polymerase chain reaction

Huang

Chan

et al. 2014

J VET Diagn Invest

View full text Add to dashboard Cite

Abstract. Infectious bronchitis virus (IBV; Avian coronavirus) causes acute respiratory and reproductive and urogenital diseases in chickens. Following sequence alignment of IBV strains, a combination of selective primer sets was designed to individually amplify the IBV wild-type and vaccine strains using a multiplex amplification refractory mutation system reverse transcription polymerase chain reaction (ARMS RT-PCR) approach. This system was shown to discriminate the IBV wildtype and vaccine strains. Moreover, an ARMS real-time RT-PCR (ARMS qRT-PCR) was combined with a high-resolution analysis (HRMA) to establish a melt curve analysis program. The specificity of the ARMS RT-PCR and the ARMS qRT-PCR was verified using unrelated avian viruses. Different melting temperatures and distinct normalized and shifted melting curve patterns for the IBV Mass, IBV H120, IBV TW-I, and IBV TW-II strains were detected. The new assays were used on samples of lung and trachea as well as virus from allantoic fluid and cell culture. In addition to being able to detect the presence of IBV vaccine and wild-type strains by ARMS RT-PCR, the IBV Mass, IBV H120, IBV TW-I, and IBV TW-II strains were distinguished using ARMS qRT-PCR by their melting temperatures and by HRMA. These approaches have acceptable sensitivities and specificities and therefore should be able to serve as options when carrying out differential diagnosis of IBV in Taiwan and China.

show abstract

“…The results strongly suggest that IBV epidemiology is very complicated in China. There are also many genotypes co-circulating in Europe, USA and other parts of the world [13][14][15][16].…”

mentioning

confidence: 99%

“…In this cluster, TC07-2 was isolated in Guangdong province in 2007 [12], the other two isolates (CK/CH/GD/ NC10 and CK/CH/GD/KP10) from 2010 were isolated in west of Guangdong province, where is far from the place of TC07-2 isolated. This novel IBV was also isolated in Japan recently (designated JP-IV), and the introduction route of this novel IBV into Japan and its prevalence are unknown [13], indicating these ''novel'' strains may spread to many chicken flocks in recent years. Because the CH VI strains continue to persist in the field and have a unique sequence compared with other strains of IBV, it is an excellent choice for studying genetic drift and recombination, may be strains of CH VI has undergone recombination event as well as extensive antigenic variation like DE072 strain [24], but need further research to testify.…”

mentioning

confidence: 99%

Phylogenetic analysis of the S1 glycoprotein gene of infectious bronchitis viruses isolated in China during 2009–2010

et al. 2011

View full text Add to dashboard Cite

As part of our ongoing surveillance program, 40 field strains of avian infectious bronchitis virus (IBV) were isolated from dead or diseased chicken flocks in different areas of China between 2009 and 2010. S1 glycoprotein genes of these strains were sequenced and analyzed with 38 strains published in GenBank. S1 genes of these isolated strains and the vaccine strains showed nucleotide homologies ranging from 65.2 to 82% and amino acid homologies ranging from 58.4 to 81.9%. Meanwhile, Chinese IBV strains isolated in this study, which were mainly nephropathogenic, could be separated into six variant lineages (CH I-CH VI), and current vaccine strains used in China formed Mass variant lineage that is evolutionarily distant from Chinese isolates. Moreover, CK/CH/GD/NC10, CK/CH/GD/KP10, and our previous isolates TC07-2 formed the CH VI lineage, showing larger evolutionary distances from other strains. Taken together, these findings suggested that various variant lineages were co-circulating in China now, and appeared to be continuously evolving, alternative indigenous vaccines indeed need for effective control of IB in China.

show abstract

A Novel Genotype of Avian Infectious Bronchitis Virus Isolated in Japan in 2009

Cited by 33 publications

References 14 publications

Genetic diversity of avian infectious bronchitis virus in China in recent years

Genetic diversity of avian infectious bronchitis virus in China in recent years

The genotyping of Infectious bronchitis virus in Taiwan by a multiplex amplification refractory mutation system reverse transcription polymerase chain reaction

Phylogenetic analysis of the S1 glycoprotein gene of infectious bronchitis viruses isolated in China during 2009–2010

Contact Info

Product

Resources

About