A Novel Genotype of &lt;i&gt;Leptospira interrogans&lt;/i&gt; Recovered from Leptospirosis Outbreak Samples from Southern Thailand

Thongdee, Metawee; Chaiwattanarungruengpaisan, Somjit; Paisin, Lekcharoen; Yimchoho, Nirandorn; Buathong, Rome; Wiriyarat, Witthawat

doi:10.7883/yoken.jjid.2019.061

Cited by 6 publications

(4 citation statements)

References 13 publications

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“…In this study, only six symptomatic dogs (2.94%) were identified positive leptospirosis by real-time PCR assay and MAT, whereas 54 to 84 of 204 dogs (26.47 to 41.18%) including the six symptomatic dogs demonstrated positive results with peptide-based ELISAs. These results are consistent with previous observations having shown that MAT, LAT and ELISA yielded a greater number of positive results than the PCR assay, culture, and microscopic demonstration [ 13 , 14 , 20 , 81 , 82 ]. Unvaccinated dogs may carry leptospires in their blood ( leptospiraemia ) for 1–10 days post infection with L. interrogans serovar Canicola and for 1–6 days post infection with L. interrogans serovar icterohaemorrhagiae [ 83 ].…”

Section: Discussionsupporting

confidence: 93%

Leptospira borgpetersenii Leucine-Rich Repeat Proteins and Derived Peptides in an Indirect ELISA Development for the Diagnosis of Canine Leptospiral Infections

Sripattanakul

Prapong²,

Kamlangdee³

et al. 2022

TropicalMed

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Domestic and stray dogs can be frequently infected by Leptospira, and thus may represent a source for transmission of this zoonotic disease in Thailand. Here, we have used peptides derived from a recombinant leucine-rich repeat (LRR) protein of Leptospira, rKU_Sej_LRR_2012M, for the development of an indirect enzyme-linked immunosorbent assay (ELISA) aimed at detecting antibodies against Leptospira interrogans, L. borgpetersenii and L. biflexa, the three major seroprevalences in Thai dogs. The rKU_Sej_LRR_2012M protein is recognized by hyperimmune sera against several leptospiral serovars. The epitope peptides of the rKU_Sej_LRR_2012M showed binding affinities with lower IC50 values than peptides of known antigenic protein LipL32. Four peptides, 2012-3T, 2012-4B, 2012-5B and pool 2012-B, were specifically recognized by rabbit hyperimmune sera against nine serovars from three Leptospira spp. The indirect peptide-based ELISAs with these four peptides were evaluated with the LipL32 ELISA by using a receiver–operator curve (ROC) analysis. All peptides had an area under the curve of ROC (AUC) greater than 0.8, and the sum of sensitivity and specificity for each peptide was greater than 1.5. The degree of agreement of 2012-3T and pool 2012-B and 2012-4B and 2012-5B peptides were in moderate-to-good levels with kappa values of 0.41–0.60 and 0.61–0.80, when compared with LipL32, respectively. This finding would suggest an excellent capability of the 2012-4B and 2012-5B peptide-based ELISAs assay for the diagnosis of canine leptospiral infections.

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Section: Discussionsupporting

confidence: 93%

Leptospira borgpetersenii Leucine-Rich Repeat Proteins and Derived Peptides in an Indirect ELISA Development for the Diagnosis of Canine Leptospiral Infections

Sripattanakul

Prapong²,

Kamlangdee³

et al. 2022

TropicalMed

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“…In comparison, the methods for detecting anti-leptospiral IgG, ELISA and LFA, gave positive results for 50 (27.17%) and 59 (32.07%) dogs, respectively. The results of this study are therefore consistent with several previous studies that demonstrated that the methods for the detection of leptospiral antibodies (MAT, LAT, ELISA) give more positive results than molecular diagnostic assays, and the culture method, respectively [5,6,[48][49][50]. The prevalence of Thai dogs that presented antibodies against Leptospira by the microscopic agglutination test (MAT), latex agglutination test (LAT), and ELISA varied from 4.3 to 89.1% [4,5,[7][8][9][10]51].…”

Section: Discussionsupporting

confidence: 92%

Leptospiral Leucine-Rich Repeat Protein-Based Lateral Flow for Assessment of Canine Leptospiral Immunoglobulin G

et al. 2022

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The recombinant, modified leucine-rich repeat protein rhKU_Sej_LRR_2271 has been suggested as a candidate for leptospiral vaccine development since it was predicted to be a transmembrane protein containing leucine-rich repeat motifs and immunogenic epitopes. The immunogenic epitopes showed binding affinities with lower IC50 values than peptides of known antigenic proteins, e.g., LipL32. Moreover, this protein was immunoreactive with hyperimmune sera against several serovars. In this study, we aimed to develop a lateral flow strip test using the rhKU_Sej_LRR_2271 protein for the detection of anti-leptospiral IgG in dogs. The lateral flow assay was performed with 184 dog plasma samples and evaluated with a culture method, 16S ribosomal RNA gene (rss) analysis real-time PCR, and LipL32 ELISA. The culture method failed to detect leptospires in the dog blood samples. Six of nine symptomatic dogs gave positive results with the real-time PCR assay. The lateral flow assay and LipL32 ELISA gave positive results with 59 and 50 dogs, respectively. The sensitivity, specificity, and accuracy of the rhKU_Sej_LRR_2271 lateral flow strip test were 70.00, 82.09, and 78.80%, respectively, when compared with LipL32 ELISA. There was a significant association between the LipL32 ELISA and the rhKU_Sej_LRR_2271 lateral flow assay. The rhKU_Sej_LRR_2271 lateral flow strip test has therefore demonstrated a good potential to detect anti-leptospiral IgG in dogs.

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“…In Thailand, some novel species of pathogenic Leptospira were reported in floodwater in Bangkok during the 2011 floods [24] and in soil samples from southern Thailand. These isolates from southern Thailand were closely related to L. ellisii [33]; they did not induce signs or symptoms of leptospirosis in a hamster model infection [31]. These novel species tend to be well adapted to their environment and are able to survive culturing even when starting from a low initial concentration [3,31,32].…”

Section: Discussionmentioning

confidence: 92%

Optimization of Culture Protocols to Isolate Leptospira spp. from Environmental Water, Field Investigation, and Identification of Factors Associated with the Presence of Leptospira spp. in the Environment

et al. 2020

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The successful culture of Leptospira spp. from the environment is challenging. Here, we optimized the isolation of Leptospira spp. from water samples spiked with different species and initial concentrations of this organism. The time periods between water sampling and the isolation process were varied (0, 2, and 4 weeks). Bacterial cultures were observed under a microscope, and cultures were graded for cell density, weekly, for 12 weeks. Most pathogenic Leptospira spp. were difficult to culture under all conditions. All conditions of water samples spiked with novel species of Leptospira subclade P1 were culture positive within 2 weeks. For Leptospira subclade P2, storing samples for 2 weeks prior to isolation resulted in more successful isolation compared with isolation after other storage conditions. For subclade S1, all samples with initial bacterial concentrations of more than 103 colonies/mL, under all storage conditions, were successfully cultured. These results suggest that storing contaminated water samples for 2 to 4 weeks in the dark at an ambient temperature prior to culturing can improve the isolation of Leptospira spp. from the samples. We implemented this protocol and collected water samples from natural sources accessed by both humans and animals. Leptospira spp. was identified in 32% (35/109) of water samples. The animal species using a water source influenced the likelihood of water samples being contaminated with Leptospira spp. Cultures of Leptospira spp. from environmental samples can provide useful information for understanding the complex interactions between humans, animals and the environment in the transmission of leptospirosis.

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A Novel Genotype of <i>Leptospira interrogans</i> Recovered from Leptospirosis Outbreak Samples from Southern Thailand

Cited by 6 publications

References 13 publications

Leptospira borgpetersenii Leucine-Rich Repeat Proteins and Derived Peptides in an Indirect ELISA Development for the Diagnosis of Canine Leptospiral Infections

Leptospira borgpetersenii Leucine-Rich Repeat Proteins and Derived Peptides in an Indirect ELISA Development for the Diagnosis of Canine Leptospiral Infections

Leptospiral Leucine-Rich Repeat Protein-Based Lateral Flow for Assessment of Canine Leptospiral Immunoglobulin G

Optimization of Culture Protocols to Isolate Leptospira spp. from Environmental Water, Field Investigation, and Identification of Factors Associated with the Presence of Leptospira spp. in the Environment

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