A novel glutathione S-transferase gene from sweetpotato, IbGSTF4, is involved in anthocyanin sequestration

Kou, Meng; Liu, Yaju; Li, Zongyun; Zhang, Yungang; Tang, Wei; Yan, Hui; Wang, Xin; Chen, Xiaoguang; Su, Zai-xing; Arisha, Mohamed Hamed; Li, Qiang; Ma, Daifu

doi:10.1016/j.plaphy.2018.12.028

Cited by 44 publications

(28 citation statements)

References 40 publications

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“…Glutathione synthetase (GS) promoted the combination of Glu and Cys to produce GSH. GSH has the ability to scavenge reactive oxygen species (ROS), by GSHPx catalyzing GSH oxidized as glutathione disulfide (GSSG) by removing H 2 O 2 ; glutathione S-transferase (GST) catalyzes the conjugation of GSH to electrophilic sites ( Ferguson et al, 2012 ; Kou et al, 2019 ). GSSG could be reduced by glutathione reductase (GR) as GSH ( Figure 5A ).…”

Section: Resultsmentioning

confidence: 99%

Transcriptomic Characterization of the Effects of Selenium on Maize Seedling Growth

et al. 2021

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Selenium (Se) is a trace mineral element in soils that can be beneficial to plants in small amounts. Although maize is among the most economically important crops, there are few reports on the effects of Se on maize seedling growth at the molecular level. In this study, the growth of maize seedlings treated with different concentrations of Na2SeO3 was investigated, and the physiological characteristics were measured. Compared with the control, a low Se concentration promoted seedling growth, whereas a high Se concentration inhibited it. To illustrate the transcriptional effects of Se on maize seedling growth, samples from control plants and those treated with low or high concentrations of Se were subjected to RNA sequencing. The differentially expressed gene (DEG) analysis revealed that there were 239 upregulated and 106 downregulated genes in the low Se treatment groups, while there were 845 upregulated and 1,686 downregulated DEGs in the high Se treatment groups. Both the Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation analyses showed a low concentration of the Se-stimulated expression of “DNA replication” and “glutathione (GSH) metabolism”-related genes. A high concentration of Se repressed the expression of auxin signal transduction and lignin biosynthesis-related genes. The real-time quantitative reverse transcription PCR (qRT-PCR) results showed that in the low Se treatment, “auxin signal transduction,” “DNA replication,” and lignin biosynthesis-related genes were upregulated 1.4- to 57.68-fold compared to the control, while, in the high Se concentration treatment, auxin signal transduction and lignin biosynthesis-related genes were downregulated 1.6- to 16.23-fold compared to the control. Based on these transcriptional differences and qRT-PCR validation, it was found that a low dosage of Se may promote maize seedling growth but becomes inhibitory to growth at higher concentrations. This study lays a foundation for the mechanisms underlying the effects of Se on maize seedling growth.

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Section: Resultsmentioning

confidence: 99%

Transcriptomic Characterization of the Effects of Selenium on Maize Seedling Growth

et al. 2021

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“…They are the two major types of plant GSTs with the most members, and both types of GSTs have typical important functions such as detoxification and stress resistance (Yang et al, 2021). In addition, phi GSTs could transport flavonoid pigments to the vacuole (Hu et al, 2016;Kou et al, 2019). In tea plants, a combination of transcription factors CsMYB75 and CsGSTF1 could promote hyperaccumulation of anthocyanin (Wei et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…The results indicated that CsGST1, CsGST2, and CsGST5 were F-type GSTs, while CsGST3 and CsGST4 were U-type GSTs (Figure 7). Motif analysis revealed that F-type CsGSTs had conserved GSH-binding C-terminal domain (GST_C) and N-terminal domain (GST_N), which were the typical structures of the plant GST family (Kou et al, 2019;Yang et al, 2021). CsGST5 was shorter than CsGST1 and CsGST2, and only GST_N domain was identified in CsGST5 protein, suggesting that CsGST5 is a partial protein of the GST family (Figure 8A).…”

Section: Glutathione S-transferase Genes In Response To Red Lightmentioning

confidence: 99%

Red Light Regulates the Metabolite Biosynthesis in the Leaves of “Huangjinya” Through Amino Acid and Phenylpropanoid Metabolisms

Song

Niu

et al. 2022

Front. Plant Sci.

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“Huangjinya” is a light-sensitive albino variety and is widely cultivated in China. It has been proved that red light could promote the vegetable growth of plants. However, the mechanism of “Huangjinya” in response to a red light is unclear. This study used high-throughput sequencing technology to analyze the transcriptome of tender shoots of “Huangjinya” under the white and red light supplement conditions. At the same time, liquid chromatography tandem mass spectrometry (LC-MS) was used to analyze metabolite changes under different light conditions. Transcriptome analysis revealed that a total of 174 differentially expressed genes (DEGs) were identified after the red light supplement. Kyoto encyclopedia of genes and genomes (KEGG) classification indicated that amino acid metabolism enriched the most DEGs. In addition, two phenylpropanoid metabolism-related genes and five glutathione S-transferase genes (CsGSTs) were found to be expressed differently. Metabolome analysis revealed that 193 differential metabolites were obtained. Being the same as transcriptome analysis, most differential metabolites were enriched in amino acids, sweet and umami tasting amino acids were increased, and bitter-tasting amino acids were decreased after the red light supplement. In summary, red light supplementary treatment may be propitious to the quality of “Huangjinya” due to its regulatory effect on amino acid metabolism. Also, CsGSTs involved phenylpropanoid metabolism contributed to tea quality changes in “Huangjinya.”

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“…They bind to anthocyanins forming the complexes GST-anthocyanins, which transport the proteins to vacuoles for storage (Pérez-Díaz et al 2016; Biała and Jasiński 2018; Lin et al 2020). The positive correlations between the expressions of CmGST1, PpGST1 and IbGSTF4 genes with the accumulation of anthocyanins has been reported in Chrysanthemum morifolium (Li et al 2021), Prunus persica (Zhao et al 2020) and Arabidopsis thaliana (Kou et al 2019), respectively. In addition, CsGSTa and CsGSTb were found to be related to proanthocyanins and avonols contents in Camellia sinensis (Liu et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Genotypic variations in GST genes reveal a regulatory role in the accumulation of caffeoylquinic acids in leafy sweet potato

Soviguidi

Long

Pan

et al. 2022

Preprint

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Leafy sweet potato is rich in caffeoylquinic acids (CQAs), including monoCQAs and diCQAs, which are important for plant resistance to environmental stresses. Knowledge about genes responsible for the accumulation of these CQAs is limited. However, the implication of glutathione S-transferase (GST) genes in the metabolism of plant secondary compounds has been reported. Yet, the mechanism of CQA-related GST proteins is not well understood. In this study, two sweet potato GST genes i.e., itb01g35330 (IbGSTTCHQD) and itb09g30700 (IbGSTT) were selected from our transcriptome database as they exhibited secondary metabolism functions based on gene ontology classification, and investigated for their association with the accumulation of CQAs. Sequence comparison of the IbGST coding regions amplified from EC16 and FS7-6 leafy sweetpotato varieties revealed some mutations at different sites in the amino acid sequences between the two varieties. Besides, the genes were highly expressed in EC16 compared to FS7-6, which corresponded to the amounts of CQAs in both varieties. The amplified sequences from EC16 were further transformed into Agrobacterium tumefaciens and subsequently introduced into Nicotiana benthamiana and Nicotiana tabacum for transient expression and transgenic transformation, respectively. Gene expression profiling in both experimental methods revealed significant increases of IbGST transcripts in transformed leaves, resulting in enhanced amount of monoCQAs compared to the wild types. However, no significant change was observed in the level of diCQAs. Moreover, the IbGSTs responded positively to salinity and oxidative stresses. Our findings suggested that IbGSTTCHQD and IbGSTT genes might be involved in the accumulation of monoCQAs in leafy sweet potato.

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A novel glutathione S-transferase gene from sweetpotato, IbGSTF4, is involved in anthocyanin sequestration

Cited by 44 publications

References 40 publications

Transcriptomic Characterization of the Effects of Selenium on Maize Seedling Growth

Transcriptomic Characterization of the Effects of Selenium on Maize Seedling Growth

Red Light Regulates the Metabolite Biosynthesis in the Leaves of “Huangjinya” Through Amino Acid and Phenylpropanoid Metabolisms

Genotypic variations in GST genes reveal a regulatory role in the accumulation of caffeoylquinic acids in leafy sweet potato

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