A novel glycoprotein from earthworm extract PvE-3: Insights of their characteristics for promoting diabetic wound healing and attenuating methylglyoxal-induced cell damage

Wang, Wenjie; Ye, Jinhong; Guo, Zishuo; Ma, Yunnan; Yang, Qilin; Zhong, Wan-Ling; Du, Shouying; Bai, Jie

doi:10.1016/j.ijbiomac.2023.124267

Cited by 5 publications

(3 citation statements)

References 57 publications

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“…The progression of cells through the G 1 , S, G 2 , and M phases is directly regulated by CDKs [ 74 , 75 ]. P53 is activated to induce cell cycle stagnation when cells are subject to minor DNA damage for self-healing [ 76 ]. Cyclin B binds to CDK1 and is regulated by phosphorylation and dephosphorylation, which promotes the G 2 /M phase transition of cells [ 77 ].…”

Section: Discussionmentioning

confidence: 99%

Hepatoprotective effect of syringin combined with costunolide against LPS-induced acute liver injury in L-02 cells via Rac1/AKT/NF-κB signaling pathway

Mao,

Tan,

Tian

et al. 2023

Aging

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Acute liver injury (ALI) leads to abnormal liver function and damage to liver cells. Syringin (syr) and costunolide (cos) are the major extracts from Dolomiaea souliei (Franch.) C.Shih ( D. souliei ), showing diverse biological functions in various biological processes. We explored the underlying hepatoprotective effects of syr+cos against LPS-induced ALI. Cell viability and proliferation were assessed using an MTT assay and immunofluorescence staining. Flow cytometry analysis was used to detect cell cycle distribution and apoptosis. ELISA was utilized to measure liver function and antioxidant stress indexes. qRT-PCR and western blotting was performed to determine mRNA and protein levels respectively. Using shRNA approach to Rac1 analyzed transcriptional targets. The results showed that syr+cos promoted L-02 cell proliferation, inhibiting the cell apoptosis and blocking cell cycle in G1 and G2/M phase. Syr+cos decreased the production of ALT, AST, LDH, MDA and ROS while increased SOD and CAT activities. Pretreated with syr+cos may decrease expressions of caspase-3,7,9, NF-κB, TNF-α proteins, Cyclin B, CDK1 and p-IκB proteins while p-IκB increased. Silencing of Rac-1 may protect the liver by increasing AKT, S473, T308 and reducing p-AKT proteins. Syr+cos exhibits anti-ALI activity via Rac1/AKT/NF-κB signaling pathway which might act as an effective candidate drug for the treatment of ALI.

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Section: Discussionmentioning

confidence: 99%

Hepatoprotective effect of syringin combined with costunolide against LPS-induced acute liver injury in L-02 cells via Rac1/AKT/NF-κB signaling pathway

Mao,

Tan,

Tian

et al. 2023

Aging

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“…32 Notably, MGO was reported to negatively affect the viability of wound-associated cells (e.g., HUVEC, fibroblasts, and HaCaT), and was commonly used to mimic the microenvironment of diabetic wounds in vitro by inducing cell damage, cytotoxicity, and apoptosis. 33,34 In this study, as human adipose tissues are mainly divided into two types: white adipose tissue (WAT) and brown adipose tissue (BAT), we individually collected sEVs from WAT and BAT by ultracentrifugation. As EVs from ADSCs have already been confirmed to be a promising treatment for wound healing therapy, 35 we isolated sEVs from ADSCs cultured in vitro to serve as a group of positive control.…”

Section: Introductionmentioning

confidence: 99%

“…The abnormally increased MGO levels in diabetic patients were found in various tissues and organs, leading to the pathogenesis of multiple diabetic complications 32 . Notably, MGO was reported to negatively affect the viability of wound‐associated cells (e.g., HUVEC, fibroblasts, and HaCaT), and was commonly used to mimic the microenvironment of diabetic wounds in vitro by inducing cell damage, cytotoxicity, and apoptosis 33,34 …”

Section: Introductionmentioning

confidence: 99%

White adipose tissue‐derived small extracellular vesicles: A new potential therapeutic reagent for accelerating diabetic wound healing

Zhang,

Jiang,

Jiang

et al. 2023

The FASEB Journal

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Small extracellular vesicles (sEVs) from adipose‐derived stem cells (ADSCs) have gained great attention and have been widely used in cell‐free therapies for treating diabetic non‐healing wounds in recent years. However, further clinical application of ADSC‐sEVs have been limited due to their unsolvable defects, including cumbersome extraction procedure, high cost, low yield, etc. Thus, we urgently need to find one therapeutic reagent that could not only accelerate diabetic wound healing as ADSC‐sEVs but also overcome these shortcomings. As the extraction process of adipose tissue‐derived sEVs (AT‐sEVs) is quite simple and labor saving, we put our focus on the efficiencies of white adipose tissue‐derived sEVs (WAT‐sEVs) and brown adipose tissue‐derived sEVs (BAT‐sEVs) in diabetic wound repair. After successfully isolating WAT‐sEVs and BAT‐sEVs by ultracentrifugation, we thoroughly characterized them and compared their diabetic wound healing capabilities both in vitro and in vivo. According to our study, AT‐sEVs possess similar competence in diabetic wound healing as compared with ADSC‐sEVs. While the effect of BAT‐sEVs is not as stable as WAT‐sEVs and ADSC‐sEVs, the repair efficiency is also slightly lower than the other two sEVs in some cases. In summary, we are the first to discover that WAT‐sEVs show great potential in diabetic wound repair. With advantages that are specific to tissue‐derived sEVs (Ti‐sEVs) such as time‐ and cost‐saving, high‐yield, and simple isolation procedure, we believe WAT‐sEVs could serve as a novel reliable cell‐free therapy for clinical diabetic wound treatment.

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Pharmacological effects of bioactive agents in earthworm extract: A comprehensive review

Zhu,

Deng,

Xie

et al. 2024

Anim Models and Exp Med

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This review compiles information from the literature on the chemical composition, pharmacological effects, and molecular mechanisms of earthworm extract (EE) and suggests possibilities for clinical translation of EE. We also consider future trends and concerns in this domain. We summarize the bioactive components of EE, including G‐90, lysenin, lumbrokinase, antimicrobial peptides, earthworm serine protease (ESP), and polyphenols, and detail the antitumor, antithrombotic, antiviral, antibacterial, anti‐inflammatory, analgesic, antioxidant, wound‐healing, antifibrotic, and hypoglycemic activities and mechanisms of action of EE based on existing in vitro and in vivo studies. We further propose the potential of EE for clinical translation in anticancer and lipid‐modifying therapies, and its promise as source of a novel agent for wound healing and resistance to antibiotic tolerance. The earthworm enzyme lumbrokinase embodies highly effective anticoagulant and thrombolytic properties and has the advantage of not causing bleeding phenomena due to hyperfibrinolysis. Its antifibrotic properties can reduce the excessive accumulation of extracellular matrix. The glycolipoprotein extract G‐90 can effectively scavenge reactive oxygen groups and protect cellular tissues from oxidative damage. Earthworms have evolved a well‐developed defense mechanism to fight against microbial infections, and the bioactive agents in EE have shown good antibacterial, fungal, and viral properties in in vitro and in vivo experiments and can alleviate inflammatory responses caused by infections, effectively reducing pain. Recent studies have also highlighted the role of EE in lowering blood glucose. EE shows high medicinal value and is expected to be a source of many bioactive compounds.

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A novel glycoprotein from earthworm extract PvE-3: Insights of their characteristics for promoting diabetic wound healing and attenuating methylglyoxal-induced cell damage

Cited by 5 publications

References 57 publications

Hepatoprotective effect of syringin combined with costunolide against LPS-induced acute liver injury in L-02 cells via Rac1/AKT/NF-κB signaling pathway

Hepatoprotective effect of syringin combined with costunolide against LPS-induced acute liver injury in L-02 cells via Rac1/AKT/NF-κB signaling pathway

White adipose tissue‐derived small extracellular vesicles: A new potential therapeutic reagent for accelerating diabetic wound healing

Pharmacological effects of bioactive agents in earthworm extract: A comprehensive review

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