A novel gradated zirconia implant material embedding bioactive ceramics: Osteoblast behavior and physicochemical assessment

Peñarrieta‐Juanito, Gabriella; Cruz, Mariana; Costa, M.M.; Miranda, G.; Marques, Joana; Magini, Ricardo de Souza; Mata, António; Souza, Júlio C. M.; Caramês, João; Silva, Filipe

doi:10.1016/j.mtla.2018.07.002

Cited by 17 publications

(17 citation statements)

References 63 publications

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“…YTZP samples with HA or βTCP were prepared with separated powders, immersed in ethanol and dispersed under a high energy ultrasonication process (40KHz, 200W) for 30s. 22 Then, YTZP granules were added to the solution while each bioactive material was in suspension for a homogeneous mixing. To avoid decantation, the alcohol volume in the solutions was controlled; then, to evaporate the ethanol, they were heated on a furnace at 60ºC for 1h and 30 min.…”

Section: Methodsmentioning

confidence: 99%

“…The maximum shear stress was determined by the ratio between maximum load and cross-section area (n=3). 22 For the vickers hardness tests, a Vickers micro-hardness tester (DuraScan, emcotest, Germany) was used on 4.9N (500g) loading for 15s; the average hardness was calculated from five indentations on each of three different groups. 21 , 22 Roughness was measured by determining Ra and Rz values, according to ISO 4287-1997, using a contact profilometer (Surftest SJ 201 from Mitutoyo, Japan), at a 4 mm evaluation length, a 0.8 mm cut-off wavelength, and a 0.25 mm/s scan speed (n=3).…”

Section: Methodsmentioning

confidence: 99%

“… 22 For the vickers hardness tests, a Vickers micro-hardness tester (DuraScan, emcotest, Germany) was used on 4.9N (500g) loading for 15s; the average hardness was calculated from five indentations on each of three different groups. 21 , 22 Roughness was measured by determining Ra and Rz values, according to ISO 4287-1997, using a contact profilometer (Surftest SJ 201 from Mitutoyo, Japan), at a 4 mm evaluation length, a 0.8 mm cut-off wavelength, and a 0.25 mm/s scan speed (n=3). Roughness average (Ra) was determined as average length between peaks and valleys and deviation from mean line, and the peak-to-valley roughness (Rz) as average vertical distance between the highest and the lowest peak.…”

Section: Methodsmentioning

confidence: 99%

“…Wettability was assessed by contact angle evaluation, using the drop shape analysis: a water droplet was deposited on the surface and, after stabilizing it, we determined four different measurements for each group by image processing using a digital goniometer (OCA 20, Data Physics, Germany). 22 …”

Section: Methodsmentioning

confidence: 99%

“…Titanium and zirconia materials – modified with these bioactive compounds, and using this strategy – have already been observed to enhance osteoblast activity. 21 , 22 Osteoblastic cell behavior has been widely characterized for bioactive calcium phosphate materials, but not much is known regarding cellular events involving soft tissue response and fibroblast adhesion.…”

Section: Introductionmentioning

confidence: 99%

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Gingival fibroblasts behavior on bioactive zirconia and titanium dental implant surfaces produced by a functionally graded technique

et al. 2020

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Adding a biological apatite layer to the implant surface enhances bone healing around the implant. Objective This study aimed to characterize the mechanical properties and test human gingival fibroblasts behavior in contact with Zirconia and Titanium bioactive-modified implant materials. Methodology 6 groups were considered: Titanium (Ti6Al4V), Ti6Al4V with 5% HA and 5% ßTCP, Zirconia (YTZP), YTZP with 5% HA and 5% ßTCP. For each group, we produced discs using a novel fabrication method for functionally graded materials, under adequate conditions for etching and grit-blasting to achieve equivalent surface microroughness among the samples. Surface roughness (Ra, Rz), water contact angle, shear bond strength, and Vickers hardness were performed. Human gingival fibroblasts immortalized by hTERT gene from the fourth passage, were seeded on discs for 14 days. Cell viability and proliferation were assessed using a resazurin-based method, and cellular adhesion and morphology using field emission gun scanning electron microscopy (FEG-SEM). After 3 days of culture, images of fluorescent nucleic acid stain were collected by confocal laser scanning microscopy (CLSM). Results Results were presented as mean ± standard deviation (SD). We compared groups using one-way ANOVA with Tukey’s post-hoc test, and significance level was set at p<0.05. After 14 days of culture, cell viability and proliferation were significantly higher in YTZP group than in other groups (p<0.05). Samples of YTZP-ßTCP presented significantly higher wettability (p<0.05); yet, we observed no improvement in cell behavior on this group. Fibroblast spreading and surface density were more evident on YTZP specimens. Adding calcium-phosphate bioactive did not alter the tested mechanical properties; however, Ti6Al4V material shear bond strength was statistically higher than other groups (p<0.05). Conclusion Adding bioactive materials did not improve soft-tissue cell behavior. When compared to other zirconia and titanium groups, pure zirconia surface improved adhesion, viability and proliferation of fibroblasts. Cell behavior seems to depend on surface chemical composition rather than on surface roughness.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Gingival fibroblasts behavior on bioactive zirconia and titanium dental implant surfaces produced by a functionally graded technique

et al. 2020

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Laser surface treatment on Yttria‐stabilized zirconia dental implants: Influence on cell behavior

et al. 2021

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Yttria-stabilized zirconia (YSZ) is being proposed as an alternative material to Titanium for dental implants due to its aesthetic and biocompatibility properties.However, is it yet to define the optimal surface treatment to improve YSZ bioactivy.Texturization is a promising approach, but the biological role of patterned YSZ surfaces in cell cultures is yet to be determined. Thus, cellular behavior of osteoblasts and fibroblasts in contact with groove-texturized YSZ surfaces was investigated. YSZ discs were groove-textured by conventional milling and Nd:YAG laser. All samples including control were sandblasted and acid-etched. Human osteoblasts and fibroblasts were cultured on discs for 14 days. Morphology and cellular adhesion were observed. Cell viability, interleukin-1β, osteopontin, collagen type I prodution, alkaline phosphatase activity, and interleukin-8 were measured. YSZ texturization by conventional milling improved osteoblasts viability and differentiation when compared to laser texturization. Fibroblasts behavior did not seem to be influenced by the texturing technique. Compared to sandblasting and acid etching currently used as gold standard for zirconia dental implants no superiority of macrotexturization was found.

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Laser-assisted manufacturing of 45S5 Bioglass-coated zirconia structured surfaces targeting medical implants: adhesive, wettability, mechanical, and bioactivity evaluation

Faria

Henriques

Souza

et al. 2021

Int J Adv Manuf Technol

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A novel gradated zirconia implant material embedding bioactive ceramics: Osteoblast behavior and physicochemical assessment

Cited by 17 publications

References 63 publications

Gingival fibroblasts behavior on bioactive zirconia and titanium dental implant surfaces produced by a functionally graded technique

Gingival fibroblasts behavior on bioactive zirconia and titanium dental implant surfaces produced by a functionally graded technique

Laser surface treatment on Yttria‐stabilized zirconia dental implants: Influence on cell behavior

Laser-assisted manufacturing of 45S5 Bioglass-coated zirconia structured surfaces targeting medical implants: adhesive, wettability, mechanical, and bioactivity evaluation

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