A Novel HA/β-TCP-Collagen Composite Enhanced New Bone Formation for Dental Extraction Socket Preservation in Beagle Dogs

Ho, Ko Ning; Salamanca, Eisner; Chang, Kuo-Song; Shih, Tsai Chin; Chang, Yu Chi; Huang, Haw Ming; Teng, Nai Chia; Lin, Che Tong; Feng, Sheng-Wei; Chang, Wei Jen

doi:10.3390/ma9030191

Cited by 18 publications

(18 citation statements)

References 43 publications

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“…Alveolar bone contraction following tooth extraction has been demonstrated in various studies [1][2][3]. To counteract this ridge resorption, biphasic bone graft materials has been investigated for ridge preservation [20,21]. The positive effect of non-crosslinked collagen membrane in ridge preservation was also demonstrated in the previous study [15].…”

Section: Discussionmentioning

confidence: 98%

Biphasic Calcium Phosphate Sphere Graft Combined with a Double-Layer Non-Crosslinked Collagen Membrane Technique for Ridge Preservation: A Randomized Controlled Animal Study

et al. 2019

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The purpose of this study was to compare the histologic and radiologic differences between single- and double-layer collagen membrane techniques in flapless ridge preservation. The mandibular fourth premolar and first molar of four beagle dogs were used in the experiment. Mesial roots of the teeth were extracted and root canal treatment was performed at the distal roots. Ridge preservation was performed at the extraction sites using synthetic bone graft material. A single layer (control group) or double layer (test group) of non-crosslinked collagen membrane was applied following bone graft application. Three months later, the animals were sacrificed and micro-computed tomography (micro-CT) and histomorphometric analyses were conducted. Nonparametric Mann–Whitney test was performed to compare between the control and test groups. The vertical difference between buccal and lingual crests of control and test groups was 1.28 ± 0.41 and 0.53 ± 0.37 mm, respectively (p = 0.026). The mineralized bone area in control and test groups was 31.48% ± 7.41% and 42.25% ± 9.73%, respectively (p = 0.041). Within the limit of this study, ridge preservation using the double-layer membrane technique showed a reduced buccal bone resorption and improved new-bone formation in the ridge compared to that using the single-layer membrane technique.

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Section: Discussionmentioning

confidence: 98%

Biphasic Calcium Phosphate Sphere Graft Combined with a Double-Layer Non-Crosslinked Collagen Membrane Technique for Ridge Preservation: A Randomized Controlled Animal Study

et al. 2019

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“…Although studies in dogs, minipigs, sheep, and non-human primates, could provide a better insight into new bone formation and scaffold effectiveness thanks to the closer resemblance to the human bone, only studies employing rat and rabbit models were found to satisfy eligibility criteria, and were, therefore, included in the present systematic review [21,[27][28][29]126]. Even though after the first step of screening, studies in dogs, sheep, and pigs were included, the full-text analysis revealed that most of these studies did not meet the selection criteria due to a reduced sample size (n < 6 animals per group) or the non-critical dimensions of the bone defects [94,100,102,103,106]. Among the papers included, the most frequently used CSD model was the CSD in rat calvaria, which is one of the most commonly used animal models for evaluating bone healing [23,127].…”

Section: Discussionmentioning

confidence: 99%

The Impact of Bioceramic Scaffolds on Bone Regeneration in Preclinical In Vivo Studies: A Systematic Review

et al. 2020

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Bioceramic scaffolds are appealing for alveolar bone regeneration, because they are emerging as promising alternatives to autogenous and heterogenous bone grafts. The aim of this systematic review is to answer to the focal question: in critical-sized bone defects in experimental animal models, does the use of a bioceramic scaffolds improve new bone formation, compared with leaving the empty defect without grafting materials or using autogenous bone or deproteinized bovine-derived bone substitutes? Electronic databases were searched using specific search terms. A hand search was also undertaken. Only randomized and controlled studies in the English language, published in peer-reviewed journals between 2013 and 2018, using critical-sized bone defect models in non-medically compromised animals, were considered. Risk of bias assessment was performed using the SYRCLE tool. A meta-analysis was planned to synthesize the evidence, if possible. Thirteen studies reporting on small animal models (six studies on rats and seven on rabbits) were included. The calvarial bone defect was the most common experimental site. The empty defect was used as the only control in all studies except one. In all studies the bioceramic materials demonstrated a trend for better outcomes compared to an empty control. Due to heterogeneity in protocols and outcomes among the included studies, no meta-analysis could be performed. Bioceramics can be considered promising grafting materials, though further evidence is needed.

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“…A commercialized cell, human osteosarcoma (MG-63, ATCC CRL-1427) osteoblast-like cell, was used for the cell test in this study. The cell has been used for testing the bioactivity and biocompatibility of various biomaterials [ 24 , 25 , 26 ]. MG-63 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) medium containing 10% heat-inactivated Fetal bovine serum (FBS), 50 U/mL penicillin, and 50 mg/mL streptomycin on a culture dish in a humidified atmosphere of 5% CO 2 and 95% air at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

Effect of Hydroxyapatite Formation on Titanium Surface with Bone Morphogenetic Protein-2 Loading through Electrochemical Deposition on MG-63 Cells

et al. 2018

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Calcium phosphate ceramics used in dentistry and orthopedics are some of the most valuable biomaterials, owing to their excellent osteoconduction, osteoinduction, and osseointegration. Osteoconduction and osteoinduction are critical targets for bone regeneration, and osseointegration is essential for any dental implantations. In this study, a hydroxyapatite (HAp) hybrid coating layer with the sequential release of bone morphogenetic protein 2 (BMP-2) was deposited onto an etched titanium substrate by electrochemical deposition. The resulting release of BMP-2 from Ti–HAp was assessed by immersing samples in a simulated buffer fluid solution. Through coculture, human osteosarcoma cell proliferation and alkaline phosphatase activity were assessed. The characteristics and effect on cell proliferation of the hybrid coatings were investigated for their functionality through X-ray diffraction (XRD) and cell proliferation assays. Findings revealed that −0.8 V vs. Ag/AgCl (3 M KCl) exhibited the optimal HAp properties and a successfully coated HAp layer. XRD confirmed the crystallinity of the deposited HAp on the titanium surface. Ti-0.8 V Ti–HAp co-coating BMP sample exhibited the highest cell proliferation efficiency and was more favorable for cell growth. A successful biocompatible hybrid coating with optimized redox voltage enhanced the osseointegration process. The findings suggest that this technique could have promising clinical applications to enhance the healing times and success rates of dental implantation.

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A Novel HA/β-TCP-Collagen Composite Enhanced New Bone Formation for Dental Extraction Socket Preservation in Beagle Dogs

Cited by 18 publications

References 43 publications

Biphasic Calcium Phosphate Sphere Graft Combined with a Double-Layer Non-Crosslinked Collagen Membrane Technique for Ridge Preservation: A Randomized Controlled Animal Study

Biphasic Calcium Phosphate Sphere Graft Combined with a Double-Layer Non-Crosslinked Collagen Membrane Technique for Ridge Preservation: A Randomized Controlled Animal Study

The Impact of Bioceramic Scaffolds on Bone Regeneration in Preclinical In Vivo Studies: A Systematic Review

Effect of Hydroxyapatite Formation on Titanium Surface with Bone Morphogenetic Protein-2 Loading through Electrochemical Deposition on MG-63 Cells

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