A novel hairless mouse model on an atopic dermatitis-prone genetic background generated by receptor-mediated transgenesis

Takada, Toyoyuki; Shitara, Hiroshi; Matsuoka, Kunie; Kojima, Erika; Ishii, Rie; Kikkawa, Yoshiaki; Taya, Choji; Karasuyama, Hajime; Kohno, Kenji; Yonekawa, Hiromichi

doi:10.1007/s11248-008-9203-6

Cited by 9 publications

(12 citation statements)

References 13 publications

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“…This means that, with the exception of coat hair, no phenotypic differences are expected between NCN24 mice and the original NC mice. We confirmed this by comparing the coat hair, the time to AD onset, the progression of AD, the serum IgE level and its change over time, and the composition of the immune cell populations in the bone marrow, spleen and thymus (Table 1) between NCN24 mice and the original NC mice (Takada et al, 2008b). As expected, there were no differences between the two strains except for coat hair.…”

Section: Details Of the Nc Modelsupporting

confidence: 73%

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Mouse Models for Atopic Dermatitis Developed in Japan

Yonekawa¹,

Takada²,

Shitara³

et al. 2012

Atopic Dermatitis - Disease Etiology and Clinical Management

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Section: Details Of the Nc Modelsupporting

confidence: 73%

“…2). Our newly developed NCN24 mice will be useful to assess drugs for AD therapy because they allow the monitoring of skin inflammation without shaving (Takada et al, 2008b). DT is highly (a) (b) (a) Approximately 9 kb of the Krt71 promoter region was amplified by PCR using genomic C57BL/6J mouse DNA.…”

Section: Details Of the Nc Modelmentioning

confidence: 99%

Mouse Models for Atopic Dermatitis Developed in Japan

Yonekawa¹,

Takada²,

Shitara³

et al. 2012

Atopic Dermatitis - Disease Etiology and Clinical Management

Self Cite

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Section: )mentioning

confidence: 99%

“…In the present study, we investigated the influence of spring water on the FA composition of skin surface lipids by using a hairless AD mouse model, NCN24 mice (transgenic mice) 9) and reversed-phase HPLC with fluorescence detection (HPLC/ FD). 10,11) The spring water examined was available from Toyotomi hot spring (Hokkaido, Japan) that is famous for being effective against AD and psoriasis, and many people from across the country has visited the watering for cure.…”

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confidence: 99%

Influence of Hot Spring Water on Fatty Acid Composition of Skin Surface Lipids in Hairless Mouse Model of Atopic Dermatitis

Nishimura

Aoyanagi

Uchino

et al. 2016

Biological & Pharmaceutical Bulletin

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When hairless NCN24 mice with atopic dermatitis (AD) were sprayed with a petroleum-containing alkaline salt spring water rich in metaboric acid and sodium bicarbonate, AD symptoms diminished. Reversedphase HPLC with fluorescence detection (HPLC/FD) and online MS revealed that fatty acid (FA) composition of the skin surface lipids was similar to that in non-AD mice compared with that in AD mice. Strong negative correlations were noted between the levels of total serum immunoglobulin E (IgE) and palmitoleic acid and between the levels of total serum IgE and branched-hexadecanoic acid. Conversely, a strong positive correlation was noted between the levels of total serum IgE and linoleic acid. The present study demonstrates that the petroleum-containing spring water alters the FA composition of skin surface lipids in AD mice, which can be used as an index to evaluate inflammation.Key words atopic dermatitis; hot spring water; fatty acid; NCN24 mouse; skin surface lipid; fluorescence detection Atopic dermatitis (AD) is a common inflammatory skin disease that has both genetic and environmental factors in its etiology.1) It is well known that lipids, fatty acids (FAs) and their metabolites play an important role in the control of inflammation in AD.2) There are many reports on the relation between FA composition and inflammation. For example, an abnormal n-6 FA composition in cheek mucosal cells of children with AD 3) and an elevation of serum linoleic acid (18 : 2n-6) level in children with AD compared with that in non-AD children 4) have been observed in studies on AD and lipid metabolism. It has also been observed that arachidonic acid (20 : 4n-6) significantly accumulated in blood cells of NC/Nga mice (an AD animal model) with AD. 2)Using NC/Nga mice, 5,6) we had previously investigated methods for assessing balneotherapy effects on pathogenesis of AD, in which the dermatitis score was used as an index to evaluate inflammation. 7,8) Comprehensive analysis of gene expression in NC/Nga mice with AD revealed significant changes in expression of several genes involved in fatty acid metabolism, such as acetyl CoA carboxylase.8) To our knowledge, however, there have been no reports on FA composition of the skin surface lipids that play an important role in moisturizing and the skin barrier system in AD mouse models. This may be due to difficulty in lipid extraction from mice with hair such as NC/Nga mice.In the present study, we investigated the influence of spring water on the FA composition of skin surface lipids by using a hairless AD mouse model, NCN24 mice (transgenic mice) 9) and reversed-phase HPLC with fluorescence detection (HPLC/ FD).10,11) The spring water examined was available from Toyotomi hot spring (Hokkaido, Japan) that is famous for being effective against AD and psoriasis, and many people from across the country has visited the watering for cure. 12) One of the features of the spring is to include petroleum-containing hot spring water that is rare in Japan. Although there is not enough scientific evide...

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“…Furthermore, he developed the inbred strain NC, which was derived from a pair of pet mice called 'Nishiki nezumi' purchased in a bird shop at about the same time. This line was called the Nishiki-Cinnamon mouse because of the distinct color of its coat, and, later, much attention was given to this line as a model for atopic dermatitis [4,24,27]. In the 1960s, Kondo started to capture wild house mice (M. m. molossinus) in the suburbs of Anjo, Aichi Prefecture, where the Faculty of Agriculture of Nagoya University was located.…”

Section: New Light On Wild Mice As a Genetic Bio-resourcementioning

confidence: 99%

Japanese Wild Mice: A Rich Resource for New Disease Models

Matsushima

2012

Exp. Anim.

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Abstract:Breeding of fancy mice has been a tradition in Japan. Recent progress in animal science has shed a new light on Japanese wild-derived mice as tools for discovery of new disease models because these mice, Mus musculus molossinus, are genetically far remote from the majority of available laboratory mice. After decades of effort, five inbred strains of mice have been established from pairs of wild mice trapped in Tohoku, northeastern Japan, namely KOR1/Stm, KOR5/Stm, KOR7/Stm, AIZ/Stm, and MAE/Stm. They carried numerous mutations, leading to a variety of diseases. During the inbreeding of KOR1, the first spontaneous mutation was found in the Apoe (apolipoprotein E) gene, and the mutant was later designated as spontaneous hyperlipidemic (SHL). Thereafter, a number of other mutations were discovered among wild-derived inbred strains, including atopic dermatitis, microphthalmia, dominant white spots, sebaceous gland abnormalities, and audible song-like vocalization. Furthermore, to examine the possible effects of the genetic background for these mutant genes, sets of congenic strains were generated, in which the mutant gene was introduced into at least 3 different strains of laboratory mice, including BALB/c and C57BL/6. These congenic strains have now been established as novel disease models. These wildderived inbred strains serve as a treasure trove for novel disease models. Most of them have been deposited in the Riken BioResource Center (BRC), and some are also available from commercial breeders.

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A novel hairless mouse model on an atopic dermatitis-prone genetic background generated by receptor-mediated transgenesis

Cited by 9 publications

References 13 publications

Mouse Models for Atopic Dermatitis Developed in Japan

Mouse Models for Atopic Dermatitis Developed in Japan

Influence of Hot Spring Water on Fatty Acid Composition of Skin Surface Lipids in Hairless Mouse Model of Atopic Dermatitis

Japanese Wild Mice: A Rich Resource for New Disease Models

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