A novel hepadnavirus in domestic dogs

Diakoudi, Georgia; Capozza, Paolo; Lanave, Gianvito; Pellegrini, Francesco; Martino, Barbara Di; Elia, Gabriella; Decaro, Nicola; Camero, Michele; Ghergo, Paola; Stasi, Fabio; Cavalli, Alessandra; Tempesta, Maria; Barrs, Vanessa R.; Beatty, Julia A.; Bányai, Krisztiàn; Catella, Cristiana; Lucente, Maria Stella; Buonavoglia, Alessio; Fusco, Giovanna; Martella, Vito

doi:10.1038/s41598-022-06842-z

Cited by 14 publications

(20 citation statements)

References 16 publications

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“…This survey revealed that 6.3% (40/635) of dog sera were DDH-positive (Diakoudi et al, 2022). Out of 40 DCH positive sera, only 23 samples have information related to hematological and serum biochemical assays which showed 47.8% (11/23) of DDH-positive dogs had an increased ALT (Diakoudi et al, 2022). This report proposes the possibility of inter-species transmission of hepadnavirus in carnivores.…”

Section: Italymentioning

confidence: 80%

“…Interestingly, a novel hepadnavirus was also detected from dogs in 2022; it was tentatively named domestic dog hepadnavirus (DDH). The genome of DDH shared 98.0 and 96.9% nucleotide (nt) identity at the full genome level with Italian and Australian DCH, respectively (Diakoudi et al, 2022). This survey revealed that 6.3% (40/635) of dog sera were DDH-positive (Diakoudi et al, 2022).…”

Section: Italymentioning

confidence: 92%

“…The genome of DDH shared 98.0 and 96.9% nucleotide (nt) identity at the full genome level with Italian and Australian DCH, respectively (Diakoudi et al, 2022). This survey revealed that 6.3% (40/635) of dog sera were DDH-positive (Diakoudi et al, 2022). Out of 40 DCH positive sera, only 23 samples have information related to hematological and serum biochemical assays which showed 47.8% (11/23) of DDH-positive dogs had an increased ALT (Diakoudi et al, 2022).…”

Section: Italymentioning

confidence: 99%

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Global Prevalence of Domestic Cat Hepadnavirus: An Emerging Threat to Cats’ Health?

et al. 2022

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Hepatitis B is an infectious hepatocellular disease of global concern caused by hepatitis B virus (HBV), which belongs to Hepadnaviridae. Recently, a novel HBV-like virus, domestic cat hepadnavirus (DCH), was detected from an immunocompromised cat with a hepatic disease in Australia. Subsequent molecular investigation by independent research groups revealed that its prevalence rates were 6.5% in Australia, 10.8% in Italy, 12.4% in Thailand, 12.3% in Malaysia, 3.08% in the United Kingdom, and 0.78% in Japan. Although the correlation between DCH infection and hepatic diseases remains to be elucidated, understanding the diversity of circulating DCH will contribute to its prevention and control in domestic cats. Herein, we summarize the current epidemiological data of DCH in these countries.

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Section: Italymentioning

confidence: 80%

Section: Italymentioning

confidence: 92%

Section: Italymentioning

confidence: 99%

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Global Prevalence of Domestic Cat Hepadnavirus: An Emerging Threat to Cats’ Health?

et al. 2022

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“…Whether a pathogenic hepadnavirus infects domestic dogs is not known. HBV DNA was detected in 10% (19/189) of sera from wild domestic dogs in Brazil [ 16 ], and in Italy, sera from 40/635 dogs (6.3%) undergoing routine laboratory testing tested positive using a qPCR designed to detect DCH [ 17 ]. A complete genome of the dog-derived hepadnavirus shared 98% nucleotide homology with DCH, and a similarly high homology (97.7 to 98.7%) was found between canine-derived hepadnaviral sequences [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

“…HBV DNA was detected in 10% (19/189) of sera from wild domestic dogs in Brazil [ 16 ], and in Italy, sera from 40/635 dogs (6.3%) undergoing routine laboratory testing tested positive using a qPCR designed to detect DCH [ 17 ]. A complete genome of the dog-derived hepadnavirus shared 98% nucleotide homology with DCH, and a similarly high homology (97.7 to 98.7%) was found between canine-derived hepadnaviral sequences [ 17 ]. Evidence of an antibody response was also detected; 13/20 DCH qPCR positive canine sera tested positive for antibodies recognizing DCH core antigen on Western blot [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Hepadnavirus DNA Is Detected in Canine Blood Samples in Hong Kong but Not in Liver Biopsies of Chronic Hepatitis or Hepatocellular Carcinoma

Choi

Chen

Carrai

et al. 2022

Viruses

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Chronic hepatitis and hepatocellular carcinoma (HCC) caused by the hepadnavirus hepatitis B virus (HBV) are significant causes of human mortality. A hepatitis-B-like virus infecting cats, domestic cat hepadnavirus (DCH), was reported in 2018. DCH DNA is hepatotropic and detectable in feline blood or serum (3.2 to 12.3%). Detection of HBV DNA has been reported in sera from 10% of free-roaming dogs in Brazil, whereas 6.3% of sera from dogs in Italy tested positive for DCH DNA by real-time quantitative PCR (qPCR). If DCH, HBV, or another hepadnavirus is hepatotropic in dogs, a role for such a virus in the etiology of canine idiopathic chronic hepatitis (CH) or HCC warrants investigation. This study investigated whether DCH DNA could be detected via qPCR in blood from dogs in Hong Kong and also whether liver biopsies from dogs with confirmed idiopathic CH or HCC contained hepadnaviral DNA using two panhepadnavirus conventional PCRs (cPCR) and a DCH-specific cPCR. DCH DNA was amplified from 2 of 501 (0.4%) canine whole-blood DNA samples. A second sample taken 6 or 7 months later from each dog tested negative in DCH qPCR. DNA extracted from 101 liver biopsies from dogs in Hong Kong or the USA, diagnosed by board-certified pathologists as idiopathic CH (n = 47) or HCC (n = 54), tested negative for DCH DNA and also tested negative using panhepadnavirus cPCRs. This study confirms that DCH DNA can be detected in canine blood by qPCR, although at a much lower prevalence than that reported previously. We identified no evidence to support a pathogenic role for a hepadnavirus in canine idiopathic CH or HCC.

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The detection and full genomic characterization of domestic catOrthohepadnavirusesfrom Türkiye

Adıgüzel,

Erdem‐Şahinkesen,

Koç

et al. 2023

Veterinary Medicine & Sci

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BackgroundDomestic cat hepadnaviruses (DCHs) have been described as a novel virus that can infect cats.ObjectiveThe aim of our study is the first identification and molecular characterizations of DCH infection in Turkish domestic cats.MethodsThe blood, organ and ascites fluid samples from 550 cats were randomly sampled. The presence of DCH nucleic acid was investigated by using both in the literature and newly designed primers.ResultsIt was found that the hepadnavirus positivity rate is 4% (22/550) in Türkiye. The full genomic characterization was performed on 13 of 22 samples, and others were characterized as nearly full genome. In this study, we highlight that whole blood samples should be also screened for DCH, not only serum samples as has frequently been done in other studies. DCH‐infected cats were also found positive (54.54%, 12/22) for Feline leukaemia virus infection. BLAST results revealed that Turkish DCHs have 86.32%–99.08% homology with strains in the GenBank database, enabling us to construct phylogenetic trees.ConclusionsAccording to this study's results, it is suggested that this infection should be added to veterinary diagnostic panels worldwide. Additionally, we suggest that our new synthesized primers for the amplification of X gene can also be used for diagnosis.

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A novel hepadnavirus in domestic dogs

Cited by 14 publications

References 16 publications

Global Prevalence of Domestic Cat Hepadnavirus: An Emerging Threat to Cats’ Health?

Global Prevalence of Domestic Cat Hepadnavirus: An Emerging Threat to Cats’ Health?

Hepadnavirus DNA Is Detected in Canine Blood Samples in Hong Kong but Not in Liver Biopsies of Chronic Hepatitis or Hepatocellular Carcinoma

The detection and full genomic characterization of domestic catOrthohepadnavirusesfrom Türkiye

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