A novel herbicide‐inducible male sterility system

Zhang, Jinhui; Zhang, Wenlu; Yen, Yang; Long, Hai; Deng, Guangbing; Pan, Zhifen; Yu, Maoqun

doi:10.1002/jsfa.4117

Cited by 3 publications

(3 citation statements)

References 20 publications

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“…To visualize the dynamics of autophagy in rice tapetal cells at various developmental stages, we generated transgenic rice lines expressing GFP-AtATG8a (GFP-ATG8) fusion protein (Yoshimoto et al 2004) under the control of ETERNAL TAPETUM1 (EAT1) and ANTHER SPECIFIC PROTEIN 6 (Osg6B/OsC6) promoters, which were mainly expressed in the tapetum during anther development stages (Ono et al 2018;Yokoi et al 1997;Zhang et al 2010) (Figure 1A). The GFP-AtATG8a fusion construct (pBI121 binary vector) was kindly provided by Dr. Koki Yoshimoto (Meiji University, Kawasaki, Japan).…”

Section: Establishment Of Transgenic Rice Plants Stably Expressing Gfp-atg8 Specifically In the Tapetummentioning

confidence: 99%

Monitoring autophagy in rice tapetal cells during pollen maturation

Hanamata

Sawada

Toh

et al. 2019

Plant Biotechnology

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We have previously shown that autophagy is required for post meiotic anther development including programmed cell death-mediated degradation of the tapetum and pollen maturation in rice. However, the spatiotemporal dynamics of autophagy in the tapetum remain poorly understood. We here established an in vivo imaging technique to analyze the dynamics of autophagy in rice tapetum cells by expressing green fluorescent protein-tagged AtATG8, a marker for autophagosomes. 3D-imaging analysis revealed that the number of autophagosomes/autophagy-related structures is extremely low at the tetrad stage (stage 8), and autophagy is dramatically induced at the uninucleate stages (stage 9-10) throughout the tapetal cells during anther development. The present monitoring system for autophagy offers a powerful tool to analyze the regulation of autophagy in rice tapetal cells during pollen maturation.

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Section: Establishment Of Transgenic Rice Plants Stably Expressing Gfp-atg8 Specifically In the Tapetummentioning

confidence: 99%

Monitoring autophagy in rice tapetal cells during pollen maturation

Hanamata

Sawada

Toh

et al. 2019

Plant Biotechnology

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“…Zhang et al (2010b) developed a novel system for creating male-sterile transgenic plants by down-regulation of the specifically expressed glyphosate tolerance CP4 EPSPS gene in male reproductive tissues. The CP4 EPSPS gene was driven by a modified CaMV 35S promoter with three tetracycline operator copies in the vicinity of the TATA box.…”

Section: Engineered Male Sterilitymentioning

confidence: 99%

Gene, protein, and network of male sterility in rice

Wang¹,

Peng²,

Ji³

et al. 2013

Front. Plant Sci.

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Rice is one of the most important model crop plants whose heterosis has been well-exploited in commercial hybrid seed production via a variety of types of male-sterile lines. Hybrid rice cultivation area is steadily expanding around the world, especially in Southern Asia. Characterization of genes and proteins related to male sterility aims to understand how and why the male sterility occurs, and which proteins are the key players for microspores abortion. Recently, a series of genes and proteins related to cytoplasmic male sterility (CMS), photoperiod-sensitive male sterility, self-incompatibility, and other types of microspores deterioration have been characterized through genetics or proteomics. Especially the latter, offers us a powerful and high throughput approach to discern the novel proteins involving in male-sterile pathways which may help us to breed artificial male-sterile system. This represents an alternative tool to meet the critical challenge of further development of hybrid rice. In this paper, we reviewed the recent developments in our understanding of male sterility in rice hybrid production across gene, protein, and integrated network levels, and also, present a perspective on the engineering of male-sterile lines for hybrid rice production.

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“…This is of greater significance when transgenic male sterility is being exploited for hybrid seed production because of the dominant nature of the induced sterility trait. Several investigators have reported the development of transgenic male sterile plants using different approaches and strategies (Yamini et al 2007;Gils et al 2008;Yamamoto et al 2008;Nizampatnam et al 2009;Bihao et al 2010;Toppino et al 2010;Zhang et al 2010). However, only a few have reported the development of appropriate transgenic lines to restore fertility in the male sterile lines (Schmülling et al 1993;Zabaleta et al 1996;Bayer and Hess 2005;Li et al 2007;Gils et al 2008;Bihao et al 2010;Toppino et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Intron hairpin and transitive RNAi mediated silencing of orfH522 transcripts restores male fertility in transgenic male sterile tobacco plants expressing orfH522

Nizampatnam

Kumar

2011

Plant Mol Biol

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The present work was aimed at developing vector construct(s) suitable for restoring fertility in transgenic male sterile tobacco plants expressing male-sterility-inducing ORFH522 in tapetal cell layer (Nizampatnam et al. Planta 229:987-1001, 2009). PTGS vectors that could produce either intron spliced hairpin RNA against the orfH522 or induce silencing of orfH522 by heterologous 3'UTR region were developed using the selected 316 bp (orf316) fragment of orfH522. The constructs were independently mobilized into Agrobacterium and used for transforming tobacco. The T(1) generation plants carrying the restorer gene cassettes in homozygous condition were identified and crossed with the male sterile transgenic tobacco plants to obtain the hybrid seeds. PCR analysis of hybrid plants indicated segregation for the sterility inducing cassette while all the plants carried the restorer cassette. Hybrid plants produced fertile pollen grains and formed normal capsules upon selfing. Further molecular analyses of these hybrid plants with RT-PCR, Northern blotting and siRNA detection, revealed that intron interrupted hairpin RNA (ihp-RNA) mediated gene silencing was more effective compared to silencing by heterologous 3'UTR (SHUTR) as indicated by the complete degradation of orfH522 transcripts and formation of higher levels of orf316 specific siRNA molecules in plants carrying ihp-RNA restorer construct. Segregation analyses of F(2) (selfed hybrid) plants confirmed the co-segregation of gene cassettes and the traits in Mendelian di-hybrid ratio (9:3:3:1). Taken together, the results established that intron hairpin and transitive RNAi mediated silencing of orfH522 transcripts restored fertility in transgenic male sterile tobacco plants expressing orfH522 and ihp-RNA was more efficient in silencing orfH522 transcripts.

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A novel herbicide‐inducible male sterility system

Abstract: BACKGROUND: Heterosis is a phenomenon that first-generation offspring perform better than their parents. Conventional breeding methods have their shortcomings. It would be optimal to construct inducible male sterile plants.

Cited by 3 publications

References 20 publications

Monitoring autophagy in rice tapetal cells during pollen maturation

Monitoring autophagy in rice tapetal cells during pollen maturation

Gene, protein, and network of male sterility in rice

Intron hairpin and transitive RNAi mediated silencing of orfH522 transcripts restores male fertility in transgenic male sterile tobacco plants expressing orfH522

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