A novel Heterosigma akashiwo (Raphidophyceae) bloom extending from a South Carolina bay to offshore waters

Kempton, Jason W.; Keppler, Charles J.; Lewitus, Alan J.; Shuler, Andrew; Wilde, Susan B.

doi:10.1016/j.hal.2007.08.003

Cited by 39 publications

(32 citation statements)

References 20 publications

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“…In the study area, the blooms of H. akashiwo occurred following a temperature increase reached to the 15.0 °C which is the lower limit of the water temperature required for activation of the cysts (Taylor and Haigh, 1993) and the bloom reached to the maximum levels in late May. Kempton et al (2008) reported the dense bloom of H. akashiwo (19.5×10 6 cells L -1 ) in the temperature level of 22.7 °C. In a H. akashiwo bloom (1.20×10 6 cells L -1 ) occurred in the Hakata Bay of Japan, water temperature was measured over 20.0 °C (Shikata et al 2008).…”

Section: Discussionmentioning

confidence: 81%

“…akashiwo is a euryhaline raphidophycean and can grow at low salinity values under the 10.0 psu, but it grows faster at salinities over 10.0 psu (Rensel et al 2010). Kempton et al (2008) stated that H. akashiwo bloom related to a rapid decrease in salinities (from 31.4 to 21.3 psu). The water salinity was measured as 16.9 psu during the dense bloom of H. akashiwo occurred in the GHE in June 2010 (Tas and Yilmaz, 2015).…”

Section: Discussionmentioning

confidence: 99%

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Spring bloom of the raphidophycean Heterosigma akashiwo in the Golden Horn Estuary at the northeast of Sea of Marmara

Dursun¹,

Taş²,

Koray³

2016

EgeJFAS

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Abstract:In the period of February-May 2012, harmful algal blooms (HABs) along with environmental factors were investigated biweekly in the Golden Horn Estuary at the northeast of the Sea of Marmara and a dense bloom of the raphidophyceaen Heterosigma akashiwo (Y.Hada) Y.Hada ex Y.Hara & M.Chihara was observed in late May. H. akashiwo abundance increased gradually from the lower estuary to the upper estuary. The dense bloom of H. akashiwo in late May occurred at the temperature of 20.2°C and salinity of 16.4 psu and its abundance reached to 10.4×10 6 cells L -1 . When compared to mid-May, the mean temperature of surface water increased 4.50°C, while salinity decreased 2.30 psu in late May. Secchi depth values decreased rapidly from the lower to the upper estuary (6.00 m to 0.50 m). It is considered that a rapid increase in temperature, a decrease in salinity, and high nutrient concentrations in combination with low water circulation at the upper estuary were the causes of the bloom of H. akashiwo. Fish mortality or other harmful effects in environment were not observed during the H. akashiwo bloom. But, these events may create a potentially toxic risk for the study area in the future.

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Section: Discussionmentioning

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Spring bloom of the raphidophycean Heterosigma akashiwo in the Golden Horn Estuary at the northeast of Sea of Marmara

Dursun¹,

Taş²,

Koray³

2016

EgeJFAS

View full text Add to dashboard Cite

show abstract

“…Blooms of H. akashiwo are usually recorded at salinities between 20 and 35, reaching an optimum at approximately 20-25 psu (Honjo, 1993;Kempton et al, 2008;Martinez et al, 2010). However, formation of H. akashiwo blooms has been also associated with freshwater runoff, usually followed by a reduction in salinity to values < 15 psu (Taylor and Haigh, 1993), and the stratification of the water column (Yamochi, 1989).…”

Section: Discussionmentioning

confidence: 99%

“…Blooms of the ichthyotoxic raphidophyte Heterosigma akashiwo (Hada) Hada occur widely in coastal ecosystems at different latitudes, where they are frequently responsible for extensive losses to farmed and wild fish stocks (O'Halloran et al, 2006;Kempton et al, 2008). Adverse and allelopathic effects of H. akahiswo are also known for various marine invertebrates including copepods, ciliates, larvae and adults of oysters, shrimps, and scallops (Connell et al, 1997;Keppler et al, 2005;Wang et al, 2006;Yu et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Recurrent blooms of Heterosigma akashiwo (Raphidophyceae) in the Piraquê Channel, Rodrigo de Freitas Lagoon, southeast Brazil

et al. 2014

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Six blooms of Heterosigma akashiwo (Raphidophyceae) were observed from March 2007 through March 2008 in the Rodrigo de Freitas Lagoon, a semi-confined eutrophic system located in Rio de Janeiro state, southeast Brazil. Vegetative cells of H. akashiwo analysed by optical and electron microscopy showed morphology as described in the literature. The blooms (2.8 × 10 4 to 4 × 10 8 cell.L -1 ) were restricted to the middle section of the Piraquê Channel, which is situated in the northeastern part of the lagoon and receives freshwater inflow. The salinity of subsurface water and the channel depth showed significant negative correlations with H. akashiwo abundances, and appeared to restrict the blooms to this compartment of the lagoon. No fish mortality was associated with the H. akashiwo blooms, nor were brevetoxins detected in a cell extract obtained from the bloom observed on 19 March 2007.Keywords: morphology, taxonomy, blooms, urban coastal lagoon, Rio de Janeiro. ) foram restritas à zona intermédia do canal Piraquê, que se situa na parte nordeste da lagoa e recebe aporte de água doce. A salinidade da sub-superfície da água e a profundidade do canal apresentaram correlação negativa significativa com a abundância de H. akashiwo e parecem determinar a formação de florações restritas a este compartimento da lagoa. Não houve mortalidade de peixes durante as florações de H. akashiwo e não foi detectada a presença de brevetoxinas em um extrato celular obtido a partir da floração observada em 19 de março de 2007. Florações recorrentes de

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“…First, both SHA and qPCR have been developed and validated for this species (Tyrrell et al 2001(Tyrrell et al , 2002Coyne et al 2005;Handy et al 2005Handy et al , 2006Demir et al 2008;Greenfield et al 2008;Portune et al 2009). Second, H. akashiwo is globally distributed and has been implicated in fish killing blooms in New Zealand (Chang et al 1990), Japan (Honjo 1992), South Africa (Bates et al 2003), South Carolina, USA (Kempton et al 2008) and Washington State, USA (Rensel et al 2010). These fish kills also have associated economic impacts.…”

Section: Comparison Of Sandwich Hybridization Assay and Quantitative mentioning

confidence: 99%

Comparison of sandwich hybridization assay and quantitative PCR for the quantification of live and preserved cultures of Heterosigma akashiwo (Raphidophyceae)

Doll

Main

Bianco

et al. 2014

Limnology & Ocean Methods

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Harmful algal bloom (HAB) species include members of several phytoplankton taxa that cause a variety of negative effects on aquatic ecosystems, human health, and economies, such as trophic accumulation of toxins, shellfish poisoning, fishery and beach closures, and others (e.g., Smayda 1997; Scholin et al. 2000;Hoagland and Scatasta 2006). Light microscopy has been traditionally used to identify and enumerate HAB species, but this approach is time consuming and accurate species identification is difficult among morphologically similar taxa and when the organism of interest is rare within a complex microbial assemblage (Anderson 1995; Anderson et al. 2005). Consequently, there has been a recent surge in the development of methods that enable rapid and accurate detection and quantification of HAB species and the substances they produce to augment research and management activities that facilitate early warning of bloom events to close fisheries or alert health officials. Examples include antibody tests, (e.g., Campbell et al. 1989;Shapiro et al. 1989), optical detection (e.g., Kirkpatrick et al. 2000;Robbins et al. 2006), molecular approaches (e.g., Anderson 1995;Groben et al. 2004), and observatory platforms (e.g., Sosik et al. 2003;See et al. 2005;Olson and Sosik 2007;Paul et al. 2007;Roman et al. 2007; Scholin et al. 2009). Despite these numerous advances, comparisons between multiple approaches are surprisingly rare. Godhe et al. (2007) describes a novel workshop that entailed cross-comparisons of methods for quantifying the "red tide" dinoflagellate Alexandrium tamarense. While results elucidated the relative performance of several techniques, varying culture conditions and use of shared equipment prevented direct comparisons, highlighting the importance for a rigorous sideby-side methods comparison accomplished by splitting a single sample followed by analyses with multiple methods. Comparison AbstractIn this study, we directly compared two molecular techniques, sandwich hybridization assay (SHA) and quantitative PCR (qPCR), for quantifying laboratory cultures of the ichthyotoxic raphidophyte Heterosigma akashiwo. To maximize comparisons, all experiments entailed raising H. akashiwo in the laboratory, generating cellular homogenates then splitting them such that for each sample, the same homogenate was analyzed using both SHA and qPCR. To verify molecular data, all experiments included cell counts using light microscopy and a Sedgewick Rafter chamber. Results of standard curves for several geographically distinct strains using SHA were similar to prior research whereas others exhibited significantly different slopes, suggesting physiological differences between isolates. Data generated by qPCR showed a high degree of correlation with SHA responses. We also investigated the use of each method for quantifying H. akashiwo samples preserved with Lugol's iodine solution. Neither SHA nor qPCR produced results that were significantly different from initial values for Lugol's preserved samples stored under refri...

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A novel Heterosigma akashiwo (Raphidophyceae) bloom extending from a South Carolina bay to offshore waters

Cited by 39 publications

References 20 publications

Spring bloom of the raphidophycean Heterosigma akashiwo in the Golden Horn Estuary at the northeast of Sea of Marmara

Spring bloom of the raphidophycean Heterosigma akashiwo in the Golden Horn Estuary at the northeast of Sea of Marmara

Recurrent blooms of Heterosigma akashiwo (Raphidophyceae) in the Piraquê Channel, Rodrigo de Freitas Lagoon, southeast Brazil

Comparison of sandwich hybridization assay and quantitative PCR for the quantification of live and preserved cultures of Heterosigma akashiwo (Raphidophyceae)

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