A novel HLA‐E allele, HLA‐E*01:12:01:01, identified in a healthy Chinese blood donor

Abstract

HLA‐E*01:12:01:01 differs from HLA‐E*01:01:01:01 by a single nucleotide in exon 5 changing 276 proline to glutamine.

Single molecule real‐time DNA sequencing of the full HLA‐E gene for 212 reference cell lines

“…Two of the novel alleles found during this study code for unique protein variants, named E*01:12:01:02 and E*01:13 (Table 2). E*01:12:01:01 was submitted by a group in China and named in the same release of the IPD‐IMGT/HLA Database 34 . E*01:12:01:02 differs from its closest reference sequence E*01:01:01:01 by the SNPs 2924C>T in intron 6 and 2009C>A in exon 5, the latter causing a substitution of proline to glutamine at position 276 in the mature protein (Table 2).…”

HLA Class I Molecules as Immune Checkpoints for NK Cell Alloreactivity and Anti-Viral Immunity in Kidney Transplantation

“…In this latter study HLA-E was typed for over 2.5 million potential stem cell donors worldwide and although only a limited 535 bp amplicon (including last part of exon 2, intron 2 and first part of exon 3) was sequenced, it has caused an explosion of new HLA-E alleles (209). Also full length sequencing of HLA-E was developed using both Sanger sequencing (210,211) and NGS (212)(213)(214)(215)(216). These full length strategies have also revealed new alleles, including alleles with polymorphism present outside of the peptide binding groove.…”