A novel HPLC method for the measurement of thiobarbituric acid reactive substances (TBARS). A comparison with a commercially available kit

Seljeskog, Esben; Hervig, Tor; Mansoor, Mohammad Azam

doi:10.1016/j.clinbiochem.2006.03.012

Cited by 113 publications

(79 citation statements)

References 32 publications

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“…Some of them [43][44][45][46][47][48] performance (based on accuracy, specificity and recovery levels) than the traditional spectrophotometric MDA-TBA test, although MDA-DNPH showed a relatively high limit of detection and a lower reproducibility at lower MDA contents in standards and samples.…”

Section: Chromatographymentioning

confidence: 99%

A review of analytical methods measuring lipid oxidation status in foods: a challenging task

Barriuso

Astiasarán

Ansorena

2012

Eur Food Res Technol

275

220

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Lipid oxidation analysis in food samples is a relevant topic since compounds generated in the process are related to undesirable sensory and biological effects. As the process is complex and depends on the type of lipid substrate, oxidation agents and environmental factors, proper measurement of lipid oxidation remains a challenging task. A great variety of methodologies have been developed and implemented so far, both for determining primary oxidation products and secondary oxidation products. Most common methods and classical procedures are described, including peroxide value, TBARS analysis and chromatography. Some other methodologies such as chemiluminescence, fluorescence emission, Raman spectroscopy, infrared spectroscopy or magnetic resonance, provide interesting and promising results, so attention must be paid to these alternative techniques in the area of food lipid oxidation analysis.

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Section: Chromatographymentioning

confidence: 99%

A review of analytical methods measuring lipid oxidation status in foods: a challenging task

Barriuso

Astiasarán

Ansorena

2012

Eur Food Res Technol

275

220

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“…Poorly specific or nonspecific methods have been used for many years to determine AGE/ALE precursors (i.e., RCCs), including 4-HNE and MDA, the most widespread technique being the thiobarbituric acid reactive substances (TBARS) assay (63 ). RCCs may be specifically evaluated, but their chemical reactivity may interfere with sample preparation, necessitating their stabilization as dinitrophenylhydrazine derivatives (11 ).…”

Section: Reactive Intermediate Compoundsmentioning

confidence: 99%

Evaluation of Nonenzymatic Posttranslational Modification–Derived Products as Biomarkers of Molecular Aging of Proteins

2010

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BACKGROUND During their biological life, proteins are exposed in a cumulative fashion to irreversible nonenzymatic, late posttranslational modifications that are responsible for their molecular aging. It is now well established that these damaged proteins constitute a molecular substratum for many dysfunctions described in metabolic and age-related diseases, such as diabetes mellitus, renal insufficiency, atherosclerosis, or neurodegenerative diseases. Accordingly, the specific end products derived from these reactions are considered potentially useful biomarkers for these diseases. CONTENT The aim of this review is to give an overview of nonenzymatic posttranslational modifications of proteins and their influence in vivo, take inventory of the analytical methods available for the measurement of posttranslational modification–derived products, and assess the potential contribution of new technologies for their clinical use as biological markers of protein molecular aging. SUMMARY Despite their clinical relevance, biomarkers of posttranslational modifications of proteins have been studied only in the context of experimental clinical research, owing to the analytical complexity of their measurement. The recent implementation in clinical chemistry laboratories of mass spectrometry–based methods that provide higher specificity and sensitivity has facilitated the measurement of these compounds. These markers are not used currently by clinicians in routine practice, however, and many challenges, such as standardization, have to be confronted before these markers can be used as efficient tools in the detection and monitoring of long-term complications of metabolic and age-related diseases.

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“…Thiobarbituric acid-malondialdehyde (MDA) adducts were separated using a method adapted from different authors ( 20,21 ) by HPLC and measured by fl uorimetry using an external calibration curve (excitation 535 nm, emission 555 nm). Hydroperoxides were quantifi ed in lipid blends according to a method adapted from Nourooz-Zahed et al ( 22 ).…”

Section: Quantifi Cation Of Free Malondialdehyde and Hydroperoxides Imentioning

confidence: 99%

Dietary oxidized n-3 PUFA induce oxidative stress and inflammation: role of intestinal absorption of 4-HHE and reactivity in intestinal cells

Awada

Soulage

Meynier

et al. 2012

Journal of Lipid Research

179

154

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A novel HPLC method for the measurement of thiobarbituric acid reactive substances (TBARS). A comparison with a commercially available kit

Cited by 113 publications

References 32 publications

A review of analytical methods measuring lipid oxidation status in foods: a challenging task

A review of analytical methods measuring lipid oxidation status in foods: a challenging task

Evaluation of Nonenzymatic Posttranslational Modification–Derived Products as Biomarkers of Molecular Aging of Proteins

Dietary oxidized n-3 PUFA induce oxidative stress and inflammation: role of intestinal absorption of 4-HHE and reactivity in intestinal cells

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