A novel hydrogen peroxide biosensor based on the immobilization of hemoglobin on three-dimensionally ordered macroporous (3DOM) gold-nanoparticle-doped titanium dioxide (GTD) film

Voltammetry at porous electrodes: A theoretical study

“…Table 1 shows recent examples of the use of porous electrodes fabricated in this manner in electrochemical sensing, where both improved sensitivity and limits of detection are often claimed. These include the detection of species such as NADH, [1][2][3] glucose, 4-6 nitrobenzene 7 and hydrogen peroxide, 8 in addition to a number of immunosensors.…”

Direct electron transfer biosensor for hydrogen peroxide carrying nanocomplex composed of horseradish peroxidase and Au-nanoparticle – Characterization and application to bienzyme systems

“…On the other hand, most of HRP-AuNP systems reported work at much negative potential such as À0.2 V vs Ag/AgCl [3,[8][9][10]. Similar working potentials for the H 2 O 2 detection were reported for an HRP-based nanowire system [27], nonenzymatic electrocatalytic systems [28][29][30][31][32], and nanomaterial-based systems with non-HRP proteins [13,[33][34][35][36][37]. In these cases, it is reasonable to consider that the catalytic redox centers do not work according to the above scheme.…”

“…Although there are several reports about the direct electron transfer systems using the combination of the AuNP and HRP [3,[8][9][10], the working electrode potentials were generally more negative, that is, they required a relatively high overpotential for the direct electron transfer to HRP. Recent papers using the combination of HRP and AuNP tend to ''hybrid'' approaches, that is, the nanoparticles are combined with organic/inorganic materials [11][12][13][14]. These approaches mainly aim to enhance the amount of enzyme and/or functional site, and improve the sensitivity and stability.…”

A new conductometric biosensor based on horseradish peroxidase immobilized on chitosan and chitosan/gold nanoparticle films

“…Electrochemical biosensors are rapid, selective, sensitive, and can be produced at low cost [2]; in addition, electrochemical biosensors have been demonstrated to be a simple, very efficient, and inexpensive method for H 2 O 2 quantification [8].…”