A novel hydrogen peroxide biosensor based on the immobilization of horseradish peroxidase onto Au-modified titanium dioxide nanotube arrays

Kafi, A.K.M.; Wu, Guosheng; Chen, Aicheng

doi:10.1016/j.bios.2008.06.004

Cited by 235 publications

(51 citation statements)

References 45 publications

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“…Pyrrole (99%) was received from ACROS Organics and purified before use by passing through a neutral Al 2 O 3 column to obtain a colourless liquid. Alumina powders of 40 sizes 0.05, 0.3 and 1.0 μm were received from CHI Instruments. Water was purified using Milli-Q water purification system.…”

Section: Methodsmentioning

confidence: 99%

“…The most suitable approach for electrode surface confinement 40 of POMs is an incorporation of them into a conducting polymer matrix, which can be done by two methods. The first approach involves the covalent modification of already synthesized polymer film at the electrode surface with the POM.…”

Section: Introductionmentioning

confidence: 99%

“…[31][32][33] Different examples of electrode modifiers that possess the advantages of electrochemical detection for hydrogen peroxide, 75 such as low-cost, reliability and simplicity, include Prussian blue, 34,35 Fe 3 O 4 , 36 vanadium-doped zirconias 37 and polyoxometalates. 38,39 Despite the high selectivity and sensitivity of enzyme-modified electrodes, 40,41 the limitation of usage of these systems is related to the difficulty in obtaining the optimal conditions and the lack of the enzymatic activity over a period of time, 42 which makes them less attractive. Owing to their inherent 5 characteristics, POMs are presented as excellent candidates for electrocatalytic reduction of hydrogen peroxide.…”

Section: Introductionmentioning

confidence: 99%

“…The SEM image reveals large 400-500 nm particles that were seen as part of a film with low separation between particles. Small particles, < 50 nm, could also be seen 40 decorating the surface of the larger particles. Fe 3+ -, Cu 2+ -POM doped polypyrrole films reveal the same surface morphology.…”

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confidence: 99%

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Transition metal ion-substituted polyoxometalates entrapped in polypyrrole as an electrochemical sensor for hydrogen peroxide

Anwar

Vagin

Laffir

et al. 2012

Analyst

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A conducting polymer was used for the immobilization of various transition metal ion-substituted Dawson-type polyoxometalates (POMs) onto glassy carbon electrodes. Voltammetric responses of films of different thicknesses were stable within the pH domain 2-7 and reveal redox processes associated with the conducting polymer, the entrapped POMs and incorporated metal ions. The resulting POM doped 10 polypyrrole films were found to be extremely stable towards redox switching between the various redox states associated with the incorporated POM. An amperometric sensor for hydrogen peroxide detection based upon the POM doped polymer films was investigated. The detection limits were 0.3 and 0.6 uM, for the Cu 2+ -and Fe 3+ -substituted POM-doped polypyrrole films respectively, with a linear region from 0.1 up to 2mM H 2 O 2 . Surface characterization of the polymer films was carried out using atomic force 15 microscopy, x-ray photoelectron spectroscopy and scanning electron microscopy. IntroductionPolyoxometalates (POMs) are a widely increasing class of inorganic early transition metal-oxide clusters. The structural and compositional diversity among this class of compounds have 20 enabled them to be promising candidates for a wide range of applications across material science, medicine, biotechnology, nanotechnology and catalysis. [1][2][3][4][5][6][7][8][9][10][11][12] The general structure of POMs consists of MO 6 units joined together by edge or corner sharing and connected tetrahedrally to a centrally bonded heteroatom 25 (usually phosphorus or silicon). One of the MO 6 units can be removed from the structure at higher pH and substituted with different metal ions allowing for controllable molecular design.Various strategies have been used for immobilization of POMs onto different surfaces, safeguarding their inherent properties. 30 These include: sol-gel technique, 13 polyoxometalates. 38,39 Despite the high selectivity and sensitivity of enzyme-modified electrodes, 40, 41 the limitation of usage of these systems is related to the difficulty in obtaining the optimal conditions and the lack of the enzymatic activity over a period of time, 42 POMs inside the polymer matrix were accessible for voltammetric studies in acidic medium. The films exhibited stable redox responses in the pH range of 2-7. Surface characterization was carried out using the AFM, XPS and SEM. Electrocatalytic activity of the films containing iron and copper-20 substituted POMs towards the reduction of hydrogen peroxide was studied by amperometry at physiological conditions (pH 6.5) and compared with the performance of the same POMs in solution 50 and immobilized onto an electrode surface via multilayer assemblies. 19 Conducting polymer-based POM-doped 25 polypyrrole films reveal the best analytical characteristics for hydrogen peroxide reduction in comparison with some other approaches. Experimental 30 MaterialsThe potassium salts of all the substituted Dawson type polyoxometalates investigated were synthesized and characterised by both ele...

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Transition metal ion-substituted polyoxometalates entrapped in polypyrrole as an electrochemical sensor for hydrogen peroxide

Anwar

Vagin

Laffir

et al. 2012

Analyst

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“…This result demonstrated that MB was an efficient mediator for transferring electrons between HRP and the electrode. HRP catalyzed hydrogen peroxide reduction according to the following schemes (Kafi et al, 2008) …”

Section: Electrochemical Characteristics Of the Developed Electrodementioning

confidence: 99%

Reagentless biosensor based on layer-by-layer assembly of functional multiwall carbon nanotubes and enzyme-mediator biocomposite

Zhou

Zhang

et al. 2011

J. Zhejiang Univ. Sci. B

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Abstract:A simple and controllable layer-by-layer (LBL) assembly method was proposed for the construction of reagentless biosensors based on electrostatic interaction between functional multiwall carbon nanotubes (MWNTs) and enzyme-mediator biocomposites. The carboxylated MWNTs were wrapped with polycations poly(allylamine hydrochloride) (PAH) and the resulting PAH-MWNTs were well dispersed and positively charged. As a water-soluble dye methylene blue (MB) could mix well with horseradish peroxidase (HRP) to form a biocompatible and negativelycharged HRP-MB biocomposite. A (PAH-MWNTs/HRP-MB) n bionanomultilayer was then prepared by electrostatic LBL assembly of PAH-MWNTs and HRP-MB on a polyelectrolyte precursor film-modified Au electrode. Due to the excellent biocompatibility of HRP-MB biocomposite and the uniform LBL assembly, the immobilized HRP could retain its natural bioactivity and MB could efficiently shuttle electrons between HRP and the electrode. The incorporation of MWNTs in the bionanomultilayer enhanced the surface coverage concentration of the electroactive enzyme and increased the catalytic current response of the electrode. The proposed biosensor displayed a fast response (2 s) to hydrogen peroxide with a low detection limit of 2.0×10 −7 mol/L (S/N=3). This work provided a versatile platform in the further development of reagentless biosensors.

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Synthesis and Analysis Applications of TiO ₂ ‐Based Nanomaterials

Sun

Fahruddin

et al. 2010

Trace Analysis With Nanomaterials

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IntroductionNanoscale titania (TiO 2 ) has a wide range of properties -from optical to electronic, chemical, and even structural -that make it an especially versatile material for performing chemical analysis. For example, TiO 2 has a fi nite resistance as a semiconductor that can change with adsorption of other species. This property has been used to build effective chemical -electrical transducers that form the basis of many chemical sensors [1 -5] . TiO 2 (formally Ti IV ) is also susceptible to oxidation and its conversion into Ti III is accompanied by a signifi cantly increased con ductance and redshift in adsorption wavelength. This behavior has been used for the analysis of certain oxidizing or reducing agents by monitoring changes in electrical [4, 6 -8] and optical properties [9 -13] . Other properties of TiO 2 nanomaterials that are favorable for trace analysis include high thermal and chemical stability, optical transparency in the visible and near -IR domain, photovoltaic properties, photo -cleaning capability, strong adsorption characteristics, and pH -dependent surface charges.Under UV illumination, the electrons of TiO 2 nanomaterials can be pumped into a conduction band, leaving holes in a valence band. This photovoltaic effect has been used for voltammetric sensing when the nanomaterials are placed in an electrical circuit under light irradiation [13 -16] . Alternatively, photo -generated electrons and holes can also act as strong reducing and oxidizing agents in the absence of an electric circuit and can participate in surface reactions. A useful application of this property, from the standpoint of analysis, is the photo -cleaning and recovery of titania -based sensors that have been poisoned by adsorption of organic contaminants [17 -19] .The strong adsorption characteristics of nanostructured TiO 2 are attributed to the high chemical activities of these surfaces and result primarily from their amphiprotic nature [20 -22] . While strong adsorption can sometimes lead to matrix interferences and poisoning issues, this property has been widely exploited to preconcentrate analytes by solid -phase extraction [20, 23 -25] and to design sensitive platforms for trace analysis. For similar reasons, the surface of nanostructured TiO 2 can be readily functionalized and charges on the surface can be altered by

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A novel hydrogen peroxide biosensor based on the immobilization of horseradish peroxidase onto Au-modified titanium dioxide nanotube arrays

Cited by 235 publications

References 45 publications

Transition metal ion-substituted polyoxometalates entrapped in polypyrrole as an electrochemical sensor for hydrogen peroxide

Transition metal ion-substituted polyoxometalates entrapped in polypyrrole as an electrochemical sensor for hydrogen peroxide

Reagentless biosensor based on layer-by-layer assembly of functional multiwall carbon nanotubes and enzyme-mediator biocomposite

Synthesis and Analysis Applications of TiO ₂ ‐Based Nanomaterials

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A novel hydrogen peroxide biosensor based on the immobilization of horseradish peroxidase onto Au-modified titanium dioxide nanotube arrays

Cited by 235 publications

References 45 publications

Transition metal ion-substituted polyoxometalates entrapped in polypyrrole as an electrochemical sensor for hydrogen peroxide

Transition metal ion-substituted polyoxometalates entrapped in polypyrrole as an electrochemical sensor for hydrogen peroxide

Reagentless biosensor based on layer-by-layer assembly of functional multiwall carbon nanotubes and enzyme-mediator biocomposite

Synthesis and Analysis Applications of TiO 2 ‐Based Nanomaterials

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Synthesis and Analysis Applications of TiO ₂ ‐Based Nanomaterials