A novelcfr-carrying Tn7transposon derivative characterized inMorganella morganiiof swine origin in China

Chen, Yanpeng; Lei, Changwei; Zuo, Lei; Kong, Linghan; Kang, Zhuang-Zhuang; Zeng, Jinxin; Zhang, Xiuzhong; Wang, Hongning

doi:10.1093/jac/dky494

Cited by 19 publications

(14 citation statements)

References 18 publications

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“…Moreover, it also decreases susceptibility to the 16-membered macrolides spiramycin and josamycin [ 2 ]. Since the first identified cfr gene in plasmid pSCFS1 from a Staphylococcus sciuri isolate, the transferable cfr gene has been detected in both Gram-positive and Gram-negative bacteria such as Staphylococcus , Enterococcus , Bacillus , Macrococcus , Jeotgalicoccus , Streptococcus , Proteus , Escherichia coli , and Morganella morganii [ 3 , 4 , 5 ]. Mobile gene elements such as insertion sequences (ISs) and plasmids can acquire antimicrobial resistance genes and play a vital role in the dissemination of cfr in both Gram-negative and Gram-positive genera [ 4 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

Rapid Increase in the IS26-Mediated cfr Gene in E. coli Isolates with IncP and IncX4 Plasmids and Co-Existing cfr and mcr-1 Genes in a Swine Farm

et al. 2021

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This paper aimed to investigate the molecular epidemiological features of the cfr gene in E. coli isolates in a typical swine farm during 2014–2017. A total of 617 E. coli isolates were screened for the cfr gene using PCR amplification. A susceptibility test, pulsed-field gel electrophoresis (PFGE), S1-PFGE, southern blotting hybridization, and the genetic context of the cfr gene were all used for analyzing all cfr-positive E. coli isolates. A conjugation experiment was conducted with the broth mating method using E. coli C600 as the recipient strain and 45 mcr-1-cfr-bearing E. coli isolates as the donor strain. Plasmids pHNEP124 and pHNEP129 were revealed by Illumina Miseq 2500. Eighty-five (13.7%) E. coli isolates were positive for the cfr gene and the prevalence of the cfr gene had significantly increased from 1.6% in 2014 to 29.1% in 2017. The Pulsed-Field Gel Electrophoresis (PFGE) analysis indicated that the spread of the cfr gene among E. coli isolates was mainly due to horizontal transfer. In addition, the cfr gene was primarily located on the plasmids between 28.8-kb to 60-kb in size, and the cfr gene was flanked by two copies of IS26 with the same orientation. Sequence analysis suggested that the plasmids pHNEP124 and pHNEP129 co-harboring the cfr and mcr-1 genes belonged to the plasmids IncP plasmid and IncX4 plasmid, respectively. In conclusion, this is the first study to report the high prevalence of the cfr gene among E. coli isolates and the first report of the complete genome sequence of IncP and IncX4 plasmids carrying the mcr-1 and cfr genes. The occurrence and dissemination of the cfr/mcr-1-carrying plasmids among E. coli isolates need further surveillance.

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Section: Introductionmentioning

confidence: 99%

Rapid Increase in the IS26-Mediated cfr Gene in E. coli Isolates with IncP and IncX4 Plasmids and Co-Existing cfr and mcr-1 Genes in a Swine Farm

et al. 2021

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“…Ongoing resistance genes or virulence factors acquisition via mobile genetic elements (MGEs) including integrative and conjugative elements (ICEs) and mobilizable genomic islands (MGIs) has promoted M. morganii to become a new clinical treatment challenge ( Flannery et al, 2009 ; Schultz et al, 2017 ). Resistance genes were mainly plasmid mediated and harbored by various transposons or integrons, such as bla KPC–2 -carrying IncP6 plasmid ( Kukla et al, 2018 ), bla OXA–181 -carrying IncN plasmid ( McGann et al, 2015 ), bla NDM–1 -carrying IncC plasmid ( Aires-de-Sousa et al, 2020 ), bla NDM–5 -carrying IncX3 plasmid ( Guo et al, 2019 ), bla IMP–27 -carrying Tn 7 transposon ( Walkty et al, 2018 ), bla CTX–M–3 -carrying Tn 6741 transposon ( Luo et al, 2020 ), cfr -carrying Tn 7 transposon ( Chen et al, 2019 ) and bla GES–5 -carrying In 1390 integron ( Moura et al, 2018 ), which have significantly contributed to the increased levels of resistance in M. morganii .…”

Section: Introductionmentioning

confidence: 99%

Clinical Molecular and Genomic Epidemiology of Morganella morganii in China

et al. 2021

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Objectives: Ongoing acquisition of antimicrobial resistance genes has made Morganella morganii a new clinical treatment challenge. Understanding the molecular epidemiology of M. morganii will contribute to clinical treatment and prevention.Methods: We undertook a 6-year clinical molecular epidemiological investigation of M. morganii from three tertiary hospitals in China since 2014. Antimicrobial susceptibility testing was performed using a VITEK-2 system. All isolates were screened for β-lactam and plasmid-mediated quinolone resistance genes by PCR. Isolates carrying carbapenem-resistant genes were subjected to whole-genome sequencing (WGS). The variation and evolution of these mobile genetic elements (MGEs) were then systematically analyzed.Results: Among all M. morganii isolates (n = 335), forty (11.9%) were recognized as multidrug resistant strains. qnrD1, aac(6′)-Ib-cr, blaTEM–104, and blaCTX–M–162 were the top four most prevalent resistance genes. Notably, phylogenomic and population structure analysis suggested clade 1 (rhierBAPS SC3 and SC5) associated with multiple resistance genes seemed to be widely spread. WGS showed a blaOXA–181-carrying IncX3 plasmid and a Proteus genomic island 2 variant carrying blaCTX–M–3, aac(6′)-Ib-cr coexisted in the same multidrug resistant strain zy_m28. Additionally, a blaIMP–1-carrying IncP-1β type plasmid was found in the strain nx_m63.Conclusion: This study indicates a clade of M. morganii is prone to acquire resistance genes, and multidrug resistant M. morganii are increasing by harboring a variety of MGEs including two newly discovered ones in the species. We should be vigilant that M. morganii may bring more extensive and challenging antimicrobial resistance issue.

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“…Tn 7 , and its derivatives had the ability to integrate into bacterial plasmids and chromosomes ( Peters, 2014 ). There were several reports of Tn 7 derivatives located in bacterial chromosomes ( Chen et al., 2018 ; Chen et al., 2019 ). To date, Tn 6726 and 40.7-kb Tn 7 -related element were the only two bla NDM -carrying Tn 7 derivatives integrated into chromosomes.…”

Section: Discussionmentioning

confidence: 99%

Chromosomal Integration of Huge and Complex blaNDM-Carrying Genetic Elements in Enterobacteriaceae

Luo

Yin

Zeng

et al. 2021

Front. Cell. Infect. Microbiol.

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In this study, a detailed genetic dissection of the huge and complex blaNDM-carrying genetic elements and their related mobile genetic elements was performed in Enterobacteriaceae. An extensive comparison was applied to 12 chromosomal genetic elements, including six sequenced in this study and the other six from GenBank. These 12 genetic elements were divided into five groups: a novel IME Tn6588; two related IMEs Tn6523 (SGI1) and Tn6589; four related ICEs Tn6512 (R391), Tn6575 (ICEPvuChnBC22), Tn6576, and Tn6577; Tn7 and its derivatives Tn6726 and 40.7-kb Tn7-related element; and two related IMEs Tn6591 (GIsul2) and Tn6590. At least 51 resistance genes, involved in resistance to 18 different categories of antibiotics and heavy metals, were found in these 12 genetic elements. Notably, Tn6576 carried another ICE Tn6582. In particular, the six blaNDM-carrying genetic elements Tn6588, Tn6589, Tn6575, Tn6576, Tn6726, and 40.7-kb Tn7-related element contained large accessory multidrug resistance (MDR) regions, each of which had a very complex mosaic structure that comprised intact or residual mobile genetic elements including insertion sequences, unit or composite transposons, integrons, and putative resistance units. Core blaNDM genetic environments manifested as four different Tn125 derivatives and, notably, two or more copies of relevant Tn125 derivatives were found in each of Tn6576, Tn6588, Tn6589, and 40.7-kb Tn7-related element. The huge and complex blaNDM-carrying genetic elements were assembled from complex transposition and homolog recombination. Firstly identified were eight novel mobile elements, including three ICEs Tn6576, Tn6577, and Tn6582, two IMEs, Tn6588 and Tn6589, two composite transposons Tn6580a and Tn6580b, and one integron In1718.

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A novelcfr-carrying Tn7transposon derivative characterized inMorganella morganiiof swine origin in China

Cited by 19 publications

References 18 publications

Rapid Increase in the IS26-Mediated cfr Gene in E. coli Isolates with IncP and IncX4 Plasmids and Co-Existing cfr and mcr-1 Genes in a Swine Farm

Rapid Increase in the IS26-Mediated cfr Gene in E. coli Isolates with IncP and IncX4 Plasmids and Co-Existing cfr and mcr-1 Genes in a Swine Farm

Clinical Molecular and Genomic Epidemiology of Morganella morganii in China

Chromosomal Integration of Huge and Complex blaNDM-Carrying Genetic Elements in Enterobacteriaceae

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