A novel image processing workflow for the in vivo quantification of skin microvasculature using dynamic optical coherence tomography

Zugaj, Didier; Chenet, A.; Petit, Laurent; Vaglio, J.; Pascual, Teresa; Piketty, Christophe; Bourdès, Valérie

doi:10.1111/srt.12442

Cited by 7 publications

(4 citation statements)

References 20 publications

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“…OCT is sensitive to the movement of blood cells, thus providing complete information about the shape of blood vessels and blood flow data that reaches the maximum depth of the skin in the middle dermis ( 21 , 39 , 45 ). OCT has a good correlation and repeatability in assessing changes in skin blood flow ( 40 , 41 ) and has the advantages of locating the dermal-epidermal junction and quantifying the size and distribution of blood vessels ( 40 ). In this study, OCT was used to detect the blood flow distribution in the MED erythema area, which measured the maximum subcutaneous depth of blood flow and calculated the total and the peak of blood flow in the test areas.…”

Section: Discussionmentioning

confidence: 99%

The role and safety of UVA and UVB in UV-induced skin erythema

Yang

Fan²,

Tan

et al. 2023

Front. Med.

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BackgroundDifferent wavelengths of ultraviolet (UV) light cause skin damage through different mechanisms. Minimal erythema dose (MED) is usually used to clinically evaluate skin sensitivity to ultraviolet radiation by inducing skin erythema using ultraviolet B (UVB) or ultraviolet A (UVA) + UVB.AimsIn this study, we detected changes in the blood flow at the MED erythema caused by UVB and UVA + UVB radiation through optical coherence tomography (OCT) to explain the role of different bands of ultraviolet rays in erythema induction.MethodsTwo MED irradiation areas on the subjects' back were irradiated with UVB alone or UVA + UVB (UVA: UVB = 8:1). The absolute energy of UVB remained the same in UVB and UVA+UVB. At 24 h after the irradiation, the changes in the blood flow in the MED area were detected using OCT.ResultsCompared with the blank control, the maximum blood flow depth, blood flow peak, and total blood flow of UVB-MED and UVA+UVB-MED were significantly increased. Notably, the maximum blood flow depth and blood flow peak of UVB-MED were higher than UVA+UVB-MED. There was no significant difference in total blood perfusion between UVA+UVB-MED and UVB-MED. Under the same UVB energy, the skin erythema caused by UVA + UVB was weaker than UVB alone.ConclusionsThe analysis of local blood flow by OCT showed that the peak and maximum depth of local blood flow caused by UVB alone were significantly higher than UVA + UVB.

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Section: Discussionmentioning

confidence: 99%

The role and safety of UVA and UVB in UV-induced skin erythema

Yang

Fan²,

Tan

et al. 2023

Front. Med.

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“…This is likely related to the pilot nature of the study, including a small number of subjects in each group; indeed, a clear trend of MVFD reduction was evident from normal/ nonspecific to early, active, and late NVC patterns, and MVFD was significantly lower in patients with a higher semiquantitative NVC score, with a higher number of abnormal shapes, and with a lower number of capillaries at nail fold. In addition, we quantified the MVFD using a software tool provided by the producer, whereas novel image processing methodologies are currently ongoing to enhance the technical capabilities of D-OCT for the accurate detection and characterization of microcirculation in the skin (Zugaj et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Nailfold Microvascular Imaging by Dynamic Optical Coherence Tomography in Systemic Sclerosis: A Case-Controlled Pilot Study

Abignano

Green

Eng

et al. 2022

Journal of Investigative Dermatology

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“…The LC-OCT system reported here generates B-scans and C-scans of 2048 ⇥ 875 pixels in 100 ms, resulting in an identical pixel rate for both modes of P ⇠ 18 MPixels/s. For comparison, the FD-OCT system commercialized by Michelson Diagnostics for skin imaging (VivoSight OCT scanner) has an A-scan rate of 20 kHz and generates B-scans of 1356 ⇥ 460 pixels [28]. Therefore its pixel rate for B-scan imaging is ⇠ 9 MPixels/s, while its pixel rate for C-scan imaging is only ⇠ 20 kPixels/s (for conventional OCT requiring a 3D image to yield C-scans, the pixel rate for C-scan imaging corresponds to the A-scan rate).…”

Section: Image Acquisition Speedmentioning

confidence: 99%

Dual-mode line-field confocal optical coherence tomography for ultrahigh-resolution vertical and horizontal section imaging of human skin in vivo

Ogien¹,

Levecq²,

Azimani³

et al. 2020

Biomed. Opt. Express

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Line-field confocal optical coherence tomography (LC-OCT) is a recently introduced technique for ultrahigh-resolution vertical section (B-scan) imaging of human skin in vivo. This work presents a new implementation of the LC-OCT technique to obtain horizontal section images (C-scans) in addition to B-scans. C-scan imaging is achieved with this dual-mode LC-OCT system using a mirror galvanometer for lateral scanning along with a piezoelectric chip for modulation of the interferometric signal. A quasi-identical spatial resolution of ⇠ 1 µm is measured for both B-scans and C-scans. The images are acquired in both modes at a rate of 10 frames per second. The horizontal field of view of the C-scans is 1.2 ⇥ 0.5 mm 2 , identical to the vertical field of view of the B-scans. The user can switch between the two modes by clicking a button. In vivo cellular-resolution imaging of human skin is demonstrated in both B-scan and C-scan modes, with the possibility to navigate within the skin tissues in real time.

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A novel image processing workflow for the in vivo quantification of skin microvasculature using dynamic optical coherence tomography

Abstract: Background: Currently, imaging technologies that can accurately assess or provide

Cited by 7 publications

References 20 publications

The role and safety of UVA and UVB in UV-induced skin erythema

The role and safety of UVA and UVB in UV-induced skin erythema

Nailfold Microvascular Imaging by Dynamic Optical Coherence Tomography in Systemic Sclerosis: A Case-Controlled Pilot Study

Dual-mode line-field confocal optical coherence tomography for ultrahigh-resolution vertical and horizontal section imaging of human skin in vivo

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