A Novel Immobilization Method for Entrapment: LentiKats®

Schlieker, M.; Vorlop, Klaus‐Dieter

doi:10.1007/978-1-59745-053-9_29

Cited by 29 publications

(20 citation statements)

References 4 publications

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“…Structural changes in the lenses were not visible under 65 °C, although above this temperature, hydrogel melting becomes noticeable. Commercial available PVA lenses, called LentiKats®, tolerate a maximum temperature from 50 °C to 55 °C and pH values between 3.1 and 8.5‎ (Schlieker and Vorlop, ). Hence, the lenses produced in this work (Figure ) display an improved thermal stability when compared with these commercial available PVA lenticular material because they endure incubation at temperatures 10 °C higher.…”

Section: Resultsmentioning

confidence: 97%

High‐affinity water‐soluble system for efficient naringinase immobilization in polyvinyl alcohol–dimethyl sulfoxide lens‐shaped particles

Nunes

Fernandes

Ribeiro

2012

J of Molecular Recognition

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Polyvinyl alcohol (PVA) is a water-soluble, biocompatible and biodegradable synthetic polymer whose application in the immobilization of biological agents for use in biocatalysis has shown promising results. This study aimed to investigate and optimize the immobilization of naringinase from Penicillium decumbens in PVA networks, targeting for the hydrolysis of naringin. Variables such as the most suitable cross-linker, catalyst, inorganic salt, co-solvents and solidification process were identified as key issues for PVA-based methods to form lens-shaped particles, while retaining high enzyme activity and stability. Major improvements were established for better and more reproducible immobilization conditions, namely, by designing a new immobilization apparatus to produce uniform lens-shaped particles. The common problems of PVA-based entrapment were significantly mitigated, through the use of selected cross-linker, glutaraldehyde (GA), and co-solvent, dimethyl sulfoxide (DMSO), which decreased the toxicity of the immobilization process and allowed the control of membrane porosity, respectively. The relevance of DMSO and GA and their interaction and effect on the swelling ratio, encapsulation efficiency and residual activity of PVA biocatalysts were established. The immobilization of naringinase in PVA under a DMSO concentration of 60%, cross-linked with 1% GA, and particle lens size of 3.5-4.0 mm, width of 100-300 µm and average particle volume of 12.5 ± 0.92 µL, allowed an encapsulation efficiency of 98.6% and an average residual activity of 87% ± 3.6%. The kinetic characterization of the immobilized naringinase showed no changes in pH profile, whereas hydrolytic activity increased up to 60 °C. Immobilization in PVA/DMSO/GA lens-shaped particles enhanced the storage stability of naringinase. Moreover, these naringinase bio-immobilizates retained a conversion rate higher than 78% after 23 runs.

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Section: Resultsmentioning

confidence: 97%

High‐affinity water‐soluble system for efficient naringinase immobilization in polyvinyl alcohol–dimethyl sulfoxide lens‐shaped particles

Nunes

Fernandes

Ribeiro

2012

J of Molecular Recognition

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“…Even though it has been mainly used for cell immobilization [8], it has been successfully applied to the immobilization of enzymepolymer composites [9,10] and enzyme aggregates [11]. The most common immobilization method is performed by mixing the biocatalyst with a solution of commercial PVA, and the promotion of gelification is accomplished by dripping the mixture over a surface, allowing for partial drying [8,12] by freezing and thawing [13] or by UV light [14], or otherwise by dripping the mixture to an extrusion solution such as boric acid or sodium sulfate to promote the cross-link between PVA molecules [15][16][17][18][19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Immobilization of Naringinase in PVA–Alginate Matrix Using an Innovative Technique

Nunes

Vila-Real

Fernandes

et al. 2009

Appl Biochem Biotechnol

101

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A synthetic polymer, polyvinyl alcohol (PVA), a cheap and nontoxic synthetic polymer to organism, has been ascribed for biocatalyst immobilization. In this work PVA-alginate beads were developed with thermal, mechanical, and chemical stability to high temperatures (<80 degrees C). The combination of alginate and bead treatment with sodium sulfate not only prevented agglomeration but produced beads of high gel strength and conferred enzyme protection from inactivation by boric acid. Naringinase from Penicillium decumbens was immobilized in PVA (10%)-alginate beads with three different sizes (1-3 mm), at three different alginate concentrations (0.2-1.0%), and these features were investigated in terms of swelling ratio within the beads, enzyme activity, and immobilization yield during hydrolysis of naringin. The pH and temperature optimum were 4.0 and 70 degrees C for the PVA-alginate-immobilized naringinase. The highest naringinase activity yield in PVA (10%)-alginate (1%) beads of 2 mm was 80%, at pH 4.0 and 70 degrees C. The Michaelis constant (K(Mapp)) and the maximum reaction velocity (V(maxapp)) were evaluated for both free (K(Mapp) = 0.233 mM; V(maxapp) = 0.13 mM min(-1)) and immobilized naringinase (K(Mapp) = 0.349 mM; V(maxapp) = 0.08 mM min(-1)). The residual activity of the immobilized enzyme was followed in eight consecutive batch runs with a retention activity of 70%. After 6 weeks, upon storage in acetate buffer pH 4 at 4 degrees C, the immobilized biocatalyst retained 90% of the initial activity. These promising results are illustrative of the potential of this immobilization strategy for the system evaluated and suggest that its application may be effectively performed for the entrapment of other biocatalysts.

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“…by immobilizing the biomass in polymeric matrixes. Immobilisation of biomass brings additional advantages such as easier separation from the treated wastewater, long retention time of the biomass in the system, and protection of the biomass from grazing by predators and from unfavourable environmental factors such as toxic substances or unsuitable pH values (Schlieker and Vorlop, 2006).…”

Section: Introductionmentioning

confidence: 99%

Comparison of denitrification at low temperature using encapsulated Paracoccus denitrificans, Pseudomonas fluorescens and mixed culture

Vacková

Srb

Stloukal

et al. 2011

Bioresource Technology

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A Novel Immobilization Method for Entrapment: LentiKats®

Cited by 29 publications

References 4 publications

High‐affinity water‐soluble system for efficient naringinase immobilization in polyvinyl alcohol–dimethyl sulfoxide lens‐shaped particles

High‐affinity water‐soluble system for efficient naringinase immobilization in polyvinyl alcohol–dimethyl sulfoxide lens‐shaped particles

Immobilization of Naringinase in PVA–Alginate Matrix Using an Innovative Technique

Comparison of denitrification at low temperature using encapsulated Paracoccus denitrificans, Pseudomonas fluorescens and mixed culture

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