A novel immunoproteomics method for identifying in vivo-induced Campylobacter jejuni antigens using pre-adsorbed sera from infected patients

Hu, Yuanqing; Shang, Yuwei; Huang, Jinlin; Wang, Yan; Ren, Fangzhe; Jiao, Yang; Pan, Zhiming; Jiao, Xinan

doi:10.1016/j.bbagen.2013.06.042

Cited by 15 publications

(16 citation statements)

References 37 publications

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“…Considering the crucial role of dnaK in antigenic mimicry and GBS, it can be concluded that individuals infected with C. jejuni strains that have sialylated LOS classes and the selected capsular serotypes as well as a high expression pro le of dnaK may be more likely to develop GBS. Furthermore, dnaK gene can be mentioned as an antigen candidate in preventive studies or as a diagnostic marker [10].…”

Section: Discussionmentioning

confidence: 99%

“…Post-infection diseases like GBS with C. jejuni have been proved to be associated with antibodies of human gangliosides. The induction of these autoantibodies is associated with molecular mimicry between human gangliosides and bacterial epitopes present at the surface of the Lipo-Oligosaccharide (LOS) [9] and the antibodies response to diarrhea is different to GBS infected by C. jejuni [10]. In addition to antiganglioside antibodies, Heat Shock Proteins (HSP) family can mediate in the autoimmune diseases.…”

Section: Introductionmentioning

confidence: 99%

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High prevalence of Campylobacter jejuni CC21 and CC257 clonal complexes in children with gastroenteritis in Iran: A global analysis

Sarhangi

Bakhshi

2020

Preprint

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Aims: The aim of this study was to assess the distribution of GBS-related lipo-oligosaccharide (LOS) classes and capsular genotypes among Iranian clinical C. jejuni strains and to assess its relation with dnaK gene expression. Moreover, a comprehensive study of C. jejuni MLST-genotypes and global comparison with peer sequences worldwide was intended.Methods: Distribution of sialylated-LOS classes and specific capsular genotypes were investigated in C.jejuni of clinical origin. The expression of dnaK in C. jejuni strains was measured by Real-Time-PCR. MLST-genotyping was performed to investigate the clonal relationship of clinical C. jejuni strains and comparison with global sequences worldwide.Results: C. jejuni HS23/36c was the predominant genotype (45%), followed by HS2 (20%), and HS19 and HS4 (each 10%). A total of 80% of isolates were assigned to LOS class B and C. Higher expression level of dnaK gene was detected in strains with HS23/36c, HS2 and HS4 capsular genotypes and sialylated LOS classes B or C in this study. MLST analysis showed that isolates were highly diverse and represented 6 different sequence types and 3 clonal complexes. ST-21 and ST-257 complexes were dominants (75%) in our C.jejuni strains. The CC21 was the largest CC in our collection which is consistent with global C.jejuni strains worldwide. No new ST and no common ST with our neighbor countries was detected in this study.Conclusion: Global analysis of MLST results demonstrated that ST-50 (CC21) was widely distributed in different countries, while ST-19 (CC21) and ST-257 (CC257) was less ubiquitously spread. Overall, CC21 and CC353 complexes were most frequent and most widely distributed clonal complexes around the world; although, CC353 was not detected in this study. This shows a picture of movement of dominant Campylobacter strains worldwide. The occurrence of identical clonal complexes with different capsular types and LOS classes in this study is consistent with genetic variation in circulating identical genotypes and their evolution toward different pathotypes probably through acquisition of different genetic elements including LOS and CPS gene clusters.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

High prevalence of Campylobacter jejuni CC21 and CC257 clonal complexes in children with gastroenteritis in Iran: A global analysis

Sarhangi

Bakhshi

2020

Preprint

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“…The disease is common in regions with extensive animal husbandry. There is a different level of infection of cattle, in some countries the infection reaches 29% [5].…”

Section: Diagnosis Of Cattle Campylobacteriosismentioning

confidence: 99%

Diagnosis of Cattle Campylobacteriosis

Ivanov¹,

Zhanserkenova²,

Nurgaliyeva³

et al. 2019

Seriâ agrarnyh nauk

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“…The in vivo gene expression profiles were evaluated by comparing the level of RNA transcription on DOC and CCDA plates with the expression of virulence genes induced with sodium deoxycholate [10,14]. The total RNA was extracted using TRIzol (TaKaRa, Dalian, China) according to the manufacturer's instructions after a 15 h culture of strain NCTC 11168 on CCDA or DOC plates.…”

Section: Real-time Pcr Analysismentioning

confidence: 99%

Use of In Vivo-Induced Antigen Technology to Identify In Vivo-Expressed Genes of Campylobacter jejuni During Human Infection

Hu¹,

Huang²,

Li³

et al. 2014

Journal of Microbiology and Biotechnology

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Campylobacter jejuni is a prevalent foodborne pathogen worldwide. Human infection by C. jejuni primarily arises from contaminated poultry meats. Genes expressed in vivo may play an important role in the pathogenicity of C. jejuni. We applied an immunoscreening method, in vivo-induced antigen technology (IVIAT), to identify in vivo-induced genes during human infection by C. jejuni. An inducible expression library of genomic proteins was constructed from sequenced C. jejuni NCTC 11168 and was then screened using adsorbed, pooled human sera obtained from clinical patients. We successfully identified 24 unique genes expressed in vivo. These genes were implicated in metabolism, molecular biosynthesis, genetic information processing, transport, and other processes. We selected six genes with different functions to compare their expression levels in vivo and in vitro using real-time RT-PCR. The results showed that the selected six genes were significantly upregulated in vivo but not in vitro. In short, these identified in vivo-induced genes may contribute to human infection of C. jejuni, some of which may be meaningful vaccine candidate antigens or diagnosis serologic markers for campylobacteriosis. IVIAT may present a significant and efficient method for understanding the pathogenicity mechanism of Campylobacter and for finding targets for its prevention and control.

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A novel immunoproteomics method for identifying in vivo-induced Campylobacter jejuni antigens using pre-adsorbed sera from infected patients

Abstract: This new analysis method may prove to be useful for identifying in vivo-induced antigens within any host infected by bacteria and will contribute to the development of new subunit vaccines.

Cited by 15 publications

References 37 publications

High prevalence of Campylobacter jejuni CC21 and CC257 clonal complexes in children with gastroenteritis in Iran: A global analysis

High prevalence of Campylobacter jejuni CC21 and CC257 clonal complexes in children with gastroenteritis in Iran: A global analysis

Diagnosis of Cattle Campylobacteriosis

Use of In Vivo-Induced Antigen Technology to Identify In Vivo-Expressed Genes of Campylobacter jejuni During Human Infection

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