A novel in vitro germination method revealed the influence of environmental variance on the pecan pollen viability

Peng, Hua-Zheng; Jin, Qun-Ying; Ye, Hualin; Zhu, Tong

doi:10.1016/j.scienta.2014.10.057

Cited by 14 publications

(14 citation statements)

References 12 publications

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“…Different techniques are cited in the literature to investigate the occurrence of viable pollens in several species and these are grouped in direct methods, such as in vitro germination [7,8,9] and in vivo [9,10,11] or indirect based in cytological parameters such as staining [9,11,12,13].…”

Section: Introductionmentioning

confidence: 99%

Pollen Viability in Eggplant Using Colorimetric and In vitro Techniques

Valadares

Lima

Nóbrega

et al. 2019

JEAI

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The causes of fruit abortion, in eggplants, may be related to the absence of viable pollen under high temperatures, common in the Northeast region of Brazil, resulting in a reduction in the number of fruits per plant and consequently in productivity. The objective of this study was to evaluate the techniques of identification of PV – pollen viability in eggplant, as well as to correlate these results with the FFI – Fruit Fixation Index, NFP – Number of Fruits per Plant and PP – Production per Plant obtained in cultivation under high temperatures. The experiment was conducted at the Federal Rural University of Pernambuco, Recife, Brazil, between September and December 2017. The experiment design was a randomized block design with four replications, in the 7 x 4 factorial scheme (7 genotypes x 4 evaluation of PV) containing four plants per experimental plot. The results showed that the different techniques used detected viable pollens in the genotypes evaluated in smaller and in larger proportions. The highest percentages were observed in the treatments with CA – Carmine Acetic and AS – Alexander Solution and these overestimated the PV (>90%). In the test with TTC – 2,3,5-triphenyltetrazolium chloride (25%), the viability estimation was equivalent to the in vitro germination test, however the genetic correlations were null and/or very low between these techniques and the FFI, NFP and PP traits. The genetic correlations between the results obtained with CA and AS with FFI, NFP and PP under conditions of high temperatures were high and positive and suggest that the selection based on PV only, except with the use of TTC solution, may be efficient for indirect selection of genotypes with high FFI, NFP and PP.

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Section: Introductionmentioning

confidence: 99%

Pollen Viability in Eggplant Using Colorimetric and In vitro Techniques

Valadares

Lima

Nóbrega

et al. 2019

JEAI

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“…In a breeding program, usually only superior parents are chosen for cross-hybridization (Ajeeshkumar and Decruse 2013 ). However, pollens of elite cultivars may not be available at hand, therefore properly stored pollen becomes an alternative to keep the desirable germplasm for cross-pollination at need in plant improvement programs (Metz et al 2000 ; Martínez-Gómez et al 2002 ; Deng and Harbaugh 2004 ; Lora et al 2006 ; Masum Akond et al 2012 ; de Souza et al 2014 ; Peng et al 2015 ; Wang et al 2015 ).…”

Section: Introductionmentioning

confidence: 99%

“…Pollen longevity or viability is defined as the stored pollen retaining its viability after long-term preservation (Vaknin and Disikowitch 2000 ). Several reports have shown that pollen stored at low temperatures was effective for long-term preservation, such as almond (Martínez-Gómez et al 2002 ), cherimoya (Lora et al 2006 ), crape myrtle (Masum Akond et al 2012 ), Arabidopsis (Bou Daher et al 2009 ), bromeliads (Parton et al 2002 ; de Souza et al 2014 ), pecan (Peng et al 2015 ), caladium (Deng and Harbaugh 2004 ), jojoba (Vaknin et al 2003 ), mango (Dutta et al 2013 ) and lily (Wang et al 2004 ). Pritchard and Prendergast ( 1989 ) used several orchid species to evaluate the pollen storage ability, including Anacamptis pyramidalis , Cymbidium elegans , C. tracyanum , Dactylorhiza fuchsii , D. maculata , Epipactis purpurata , Gymnadenia conopsea , Listera ovata , Orchis mascula , O. morio , and Spiranthes spiralis .…”

Section: Introductionmentioning

confidence: 99%

Phalaenopsis pollinia storage at sub-zero temperature and its pollen viability assessment

et al. 2018

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BackgroundIn a breeding program, usually only superior parents are chosen for cross hybridization. Pollens of elite cultivars may not be available at hand. Properly stored pollens provide an opportunity for cross hybridization at unavailable time.ResultsPollen of a Phalaenopsis hybrid was evaluated for the storage ability at different temperatures, including room temperature, 4, − 20, and − 80 °C for up to 96 weeks. The viability of pollen was assessed by TTC staining, in vitro germination and hand pollination during and after storage. Pollen stored at all temperatures for 4 weeks remained viable and capable of successful pollination. Pollen lost its viability after 4 weeks at room temperature. Pollen remains viable after 40 weeks at 4 °C, and after 96 weeks at both − 20 and − 80 °C of storage. Viable pollen could be successfully pollinated to the female parent at all effective storage conditions and produced seeds.ConclusionsOur results indicate that Phalaenopsis pollen can be stored at 4 °C up to 40 weeks for short-term purpose. For long-term storage, pollen can be kept at both − 20 and −80 °C.

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“…Determination of pollen viability can be done using direct methods, such as in vitro (Peng et al 2015) and in vivo germination (Soares et al 2014;Souza et al 2015), or through indirect methods based on cytological parameters, such as coloration in response to staining (Melloni et al 2013;Souza et al 2015). Although the histochemical test is quick, easy and inexpensive, it should not be the only method used to estimate pollen viability, since it does not supply information on the germinative capacity.…”

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confidence: 99%

Viability of pollen grains of tetraploid banana

et al. 2016

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Obtaining banana tetraploid cultivars from triploid strains results in total or partial reestablishment of fertility, allowing the occurrence of some fruits with seeds, a feature that is undesirable from a marketing perspective. The objective of this study was to assess the viability of pollen of 12 banana tetraploid hybrids (AAAB) by means of in vitro germination and two histochemical tests (acetocarmine and 2,3,5-triphenyltetrazolium chloride). The pollen tube growth was evaluated by germinating grains in three culture media -M1: Received: Jul. 23, 2015 -Accepted: Sept. 20, 2015 for the genotypes YB42-17 and Caprichosa, which were 76 and 70%, respectively. However, the in vitro germination rate was lower than 50% for all the genotypes, except for the hybrids YB42-17 (M1) and YB42-47 (M1). The medium M1 provided the greatest germination percentage and pollen tube growth. Among the genotypes assessed, YB42-47 presented the highest germination rate (61.5%) and tube length (5.0 mm). On the other hand, the Vitória cultivar had the lowest germination percentage (8.2%) in medium M1. Studies of meiosis can shed more light on the differences observed in the evaluated tetraploids, since meiotic irregularities can affect pollen viability.

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A novel in vitro germination method revealed the influence of environmental variance on the pecan pollen viability

Cited by 14 publications

References 12 publications

Pollen Viability in Eggplant Using Colorimetric and In vitro Techniques

Pollen Viability in Eggplant Using Colorimetric and In vitro Techniques

Phalaenopsis pollinia storage at sub-zero temperature and its pollen viability assessment

Viability of pollen grains of tetraploid banana

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