A novel in vivo atlas of human hippocampal subfields using high-resolution 3T magnetic resonance imaging

Winterburn, Julie L.; Pruessner, Jens C.; Chavez, Sofia; Schira, Mark M.; Lobaugh, Nancy J.; Voineskos, Aristotle N.; Chakravarty, M. Mallar

doi:10.1016/j.neuroimage.2013.02.003

Cited by 236 publications

(309 citation statements)

References 43 publications

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“…The entire anterior-posterior length of one hemisphere of each subject was resegmented, whereas other groups with higher reliabilities segment only a few coronal slices 23,33,37 .The subfield with the lowest kappa (0.64) is the CA2/CA3, which is a small, thin structure. It has previously been shown that the intra-rater error for all subfields in this protocol is higher than a simulated 0.3 mm translational error in every cardinal direction, or a simulated 1% expansion/shrinkage of labels 34 . In other words, the manual resegmentation error is smaller than introducing a small systematic error, which supports the high manual reproducibility of the protocol.…”

Section: Discussionmentioning

confidence: 76%

“…This protocol was developed for high-resolution MR images such as those used to illustrate the protocol steps in this manuscript 26,34 . Highresolution images were acquired on a 3T scanner by taking advantage of long scan times and image averaging.…”

Section: Discussionmentioning

confidence: 99%

“…All subjects provided written, informed consent for data acquisition and sharing. For details about the acquisition sequence used to collect these images, please refer to Winterburn et al, 2013 andPark et al, 2014. 26,34 Images for all five subjects…”

Section: Study Participantsmentioning

confidence: 99%

“…This manuscript provides detailed written and video instructions for reliably implementing the hippocampal subfield segmentation protocol described previously by Winterburn and colleagues 34 on high-resolution 3T MR images. The protocol has been implemented on five images of healthy controls for the whole hippocampus and five hippocampal subfields (CA1, CA2/CA3, CA4/dentate gyrus, strata radiatum/lacunosum/ moleculare, and subiculum).…”

Section: Introductionmentioning

confidence: 99%

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High-resolution <em>In Vivo</em> Manual Segmentation Protocol for Human Hippocampal Subfields Using 3T Magnetic Resonance Imaging

Winterburn

Pruessner

Sofia

et al. 2015

JoVE

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The human hippocampus has been broadly studied in the context of memory and normal brain function and its role in different neuropsychiatric disorders has been heavily studied. While many imaging studies treat the hippocampus as a single unitary neuroanatomical structure, it is, in fact, composed of several subfields that have a complex three-dimensional geometry. As such, it is known that these subfields perform specialized functions and are differentially affected through the course of different disease states. Magnetic resonance (MR) imaging can be used as a powerful tool to interrogate the morphology of the hippocampus and its subfields. Many groups use advanced imaging software and hardware (>3T) to image the subfields; however this type of technology may not be readily available in most research and clinical imaging centers. To address this need, this manuscript provides a detailed step-by-step protocol for segmenting the full anterior-posterior length of the hippocampus and its subfields: cornu ammonis (CA) 1, CA2/CA3, CA4/dentate gyrus (DG), strata radiatum/lacunosum/moleculare (SR/SL/SM), and subiculum. This protocol has been applied to five subjects (3F, 2M; age 29-57, avg. 37). Protocol reliability is assessed by resegmenting either the right or left hippocampus of each subject and computing the overlap using the Dice's kappa metric. Mean Dice's kappa (range) across the five subjects are: whole hippocampus, CA1, CA2/CA3,); CA4/dentate gyrus, 0.83 (0.81-0.85); strata radiatum/lacunosum/moleculare, 0.71 (0.68-0.73); and subiculum 0.75 (0.72-0.78). The segmentation protocol presented here provides other laboratories with a reliable method to study the hippocampus and hippocampal subfields in vivo using commonly available MR tools. Video LinkThe video component of this article can be found at

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Section: Discussionmentioning

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Section: Study Participantsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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High-resolution <em>In Vivo</em> Manual Segmentation Protocol for Human Hippocampal Subfields Using 3T Magnetic Resonance Imaging

Winterburn

Pruessner

Sofia

et al. 2015

JoVE

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“…Although we do not believe that this would materially affect the results presented here, we cannot exclude this as a possibility and further studies using consistent imaging parameters would be required to confirm our findings. Sixthly, the images used were of limited resolution compared with the highresolution temporal lobe imaging which is achievable [Winterburn et al, 2013]. Given that the hippocampi are relatively small structures it may be that using higher resolution scans would enable the detection of more subtle shape differences between groups.…”

Section: Discussionmentioning

confidence: 99%

O5‐01‐02: Differential Hippocampal Shapes in Posterior Cortical Atrophy Patients: A Comparison With Control and Typical Ad Subjects

Manning

Macdonald

Leung³

et al. 2014

Alzheimer's & Dementia

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Posterior cortical atrophy (PCA) is a neurodegenerative syndrome characterized by predominant visual deficits and parieto-occipital atrophy, and is typically associated with Alzheimer's disease (AD) pathology. In AD, assessment of hippocampal atrophy is widely used in diagnosis, research, and clinical trials; its utility in PCA remains unclear. Given the posterior emphasis of PCA, we hypothesized that hippocampal shape measures may give additional group differentiation information compared with wholehippocampal volume assessments. We investigated hippocampal volume and shape in subjects with PCA (n 5 47), typical AD (n 5 29), and controls (n 5 48). Hippocampi were outlined on MRI scans and their 3D meshes were generated. We compared hippocampal volume and shape between disease groups. Mean adjusted hippocampal volumes were 8% smaller in PCA subjects (P < 0.001) and~22% smaller in tAD subject (P < 0.001) compared with controls. Significant inward deformations in the superior hippocampal tail were observed in PCA compared with controls even after adjustment for hippocampal volume. Inward deformations in large areas of the hippocampus were seen in tAD subjects compared with controls and PCA subjects, but only localized shape differences remained after adjusting for hippocampal volume. The shape differences observed, even allowing for volume differences, suggest that PCA and tAD are each associated

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Exploring the cognitive assessment potential of MRI‐based volumetric hippocampal segmentation in Parkinson's disease

Tarhan,

Atalay,

Buz Yaşar

et al. 2024

Brain and Behavior

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PurposeTo investigate the potential of magnetic resonance imaging (MRI)‐based total and segmental hippocampus volume analysis in the assessment of cognitive status in Parkinson's disease (PD).MethodsWe divided participants into three groups Group A‐Parkinson patients (Pp) with normal cognitive status (n = 25), Group B‐Pp with dementia (n = 17), and Group C‐healthy controls (n = 37). Three‐dimensional T1W Fast Spoiled Gradient Recalled Echo images were used for Volbrain hippocampus subfield segmentation. We used the “Winterburn” protocol, which divides the hippocampus into five segments, Cornu Ammonis (CA),CA2/CA3, CA4/dentate gyrus, stratum radiatum, lacunosum, and moleculare, and subiculum.ResultsA total of 79 participants were included in the study, consisting of 42 individuals with PD (64.2% male) and 37 healthy controls (54.1% male). The mean age of PD was 60.9 ± 10.7 years and the mean age of control group was 59.27 ± 12.3 years. Significant differences were found in total hippocampal volumes between Group A and B (p = .047. Statistically significant group differences were found in total, right, and left CA1 volumes (analysis of variance [ANOVA]: F(2,76) = 8.098, p = .001; F(2,76) = 7.628, p = .001; F(2,76) = 5.084, p = .008, respectively), as well as in total subiculum volumes (ANOVA: F(2,76) = 4.368, p = .016). Post hoc tests showed that total subiculum volume was significantly lower in individuals with normal cognitive status (0.474 ± 0.116 cm3) compared to healthy controls (0.578 ± 0.151 cm3, p = .013).ConclusionVolumetric hippocampal MRI can be used to assess the cognitive status of Pp. Longitudinal studies that evaluate Pp who progress from normal cognition to dementia are required to establish a causal relationship.

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A novel in vivo atlas of human hippocampal subfields using high-resolution 3T magnetic resonance imaging

Cited by 236 publications

References 43 publications

High-resolution <em>In Vivo</em> Manual Segmentation Protocol for Human Hippocampal Subfields Using 3T Magnetic Resonance Imaging

High-resolution <em>In Vivo</em> Manual Segmentation Protocol for Human Hippocampal Subfields Using 3T Magnetic Resonance Imaging

O5‐01‐02: Differential Hippocampal Shapes in Posterior Cortical Atrophy Patients: A Comparison With Control and Typical Ad Subjects

Exploring the cognitive assessment potential of MRI‐based volumetric hippocampal segmentation in Parkinson's disease

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