A novel integrin α5β1 antagonistic peptide, A5-1, screened by Protein Chip system as a potent angiogenesis inhibitor

Kim, Eung-Yoon; Bang, Ji Young; Chang, Soo-Ik; Kang, In‐Cheol

doi:10.1016/j.bbrc.2008.10.166

Cited by 22 publications

(12 citation statements)

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“…Combinatorial approaches to peptides as protein-site mimetics have been discussed in a recent review. 56 Such screens of either random [57][58][59][60][61] or biased peptide libraries 22,38,62 of peptides of up to 20 amino acids in length, 63 may identify potent peptidic inhibitors that are not necessarily similar to native ligands of the protein, but nevertheless efficiently inhibit the proteinprotein interaction of interest. Lead inhibitory peptides, whether based on a known binding partner or obtained from a library screen, often serve as merely the first step in developing an inhibitor.…”

Section: Finding An Optimal Synthetic Peptide By a Wide-scale Screenmentioning

confidence: 99%

Peptidic modulators of protein‐protein interactions: Progress and challenges in computational design

Rubinstein

Niv

2009

Biopolymers

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With the decline in productivity of drug‐development efforts, novel approaches to rational drug design are being introduced and developed. Naturally occurring and synthetic peptides are emerging as novel promising compounds that can specifically and efficiently modulate signaling pathways in vitro and in vivo. We describe sequence‐based approaches that use peptides to mimic proteins in order to inhibit the interaction of the mimicked protein with its partners. We then discuss a structure‐based approach, in which protein‐peptide complex structures are used to rationally design and optimize peptidic inhibitors. We survey flexible peptide docking techniques and discuss current challenges and future directions in the rational design of peptidic inhibitors. © 2009 Wiley Periodicals, Inc. Biopolymers 91: 505–513, 2009.This article was originally published online as an accepted preprint. The “Published Online”date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com

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Section: Finding An Optimal Synthetic Peptide By a Wide-scale Screenmentioning

confidence: 99%

Peptidic modulators of protein‐protein interactions: Progress and challenges in computational design

Rubinstein

Niv

2009

Biopolymers

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“…A5-1, a peptide selected through proteoChip-based library screening, is effective as an angiogenistic inhibitior against integrin α5β1 10 . Korean medicinal plant extracts chosen under the same methodology have been verified for their anti-angiogenic functions 11 .…”

Section: Introductionmentioning

confidence: 99%

Analysis of anti-angiogenic mechanism of HangAmDan-B (HAD-B), a Korean traditional medicine, using antibody microarray chip

et al. 2010

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The inhibitory effects of the water extract of HangAmDan-B (WEHAD-B), which is a crude extract of eight Korean medicinal animals and plants on bFGF-induced neovascularization were investigated. WEHAD-B significantly prevented bFGF-induced HUVE cell proliferation, adhesion, migration, and capillary-like tubular network formation. Half-maximal inhibition of proliferation on the endothelial cells by WEHAD-B was observed at a concentration of approximately 250 μg/mL. Our antibody microarray-based ProteoChip data showed that WEHAD-B increased the expression of STAT1 and Rb2, which are involved in cell growth, apoptosis, and controlling the cell cycle in bFGF-induced HUVECs. These results indicate that the inhibition of bFGF-induced angiogenesis by WEHAD-B may be due to upregulation of cell signaling proteins, STAT1 and Rb2. The blood vessel formation in a chick chorioallantoic membrane (CAM) treated with WEHAD-B was markedly reduced in length compared with a PBS-treated control group. Taken together, these data suggest that antibody-arrayed ProteoChip technology may be an useful tool for determining molecular mechanism of natural products in biological samples.

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“…59 They can be incubated with cells or receptors, typically in a high-throughput fashion such as in a 96-well plate or a microarray, and numerous readouts for binding exist. For example, screens can be made for binding versus competitor fluorescently tagged natural ligands (looking for loss of fluorescence) 63,64 or using biotinylated peptide and streptavidin–horseradish peroxidase (HRP) as a detection reagent. 65 PS-SPCLs are relatively easy and inexpensive to synthesize in large numbers.…”

Section: Peptide Libraries Used To Select Cell-binding Peptidesmentioning

confidence: 99%

“…They are typically incubated with cells or receptors in a high-throughput fashion, such as in a 96-well plate or a microarray, and the readout for binding is varied. Screens can be made for binding versus competitor natural ligands that are fluorescently tagged (looking for loss of fluorescence) 64,87 or can use biotinylated peptide and streptavidin–HRP as a detection reagent. 65 Other screens have used specific cellular effects as the readout.…”

Section: Using Peptide Libraries To Isolate Cell-binding Peptidesmentioning

confidence: 99%

Combinatorial Peptide Libraries: Mining for Cell-Binding Peptides

2013

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A novel integrin α5β1 antagonistic peptide, A5-1, screened by Protein Chip system as a potent angiogenesis inhibitor

Cited by 22 publications

References 21 publications

Peptidic modulators of protein‐protein interactions: Progress and challenges in computational design

Peptidic modulators of protein‐protein interactions: Progress and challenges in computational design

Analysis of anti-angiogenic mechanism of HangAmDan-B (HAD-B), a Korean traditional medicine, using antibody microarray chip

Combinatorial Peptide Libraries: Mining for Cell-Binding Peptides

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