A novel interleukin-1 receptor-associated kinase-4 from thick shell mussel Mytilus coruscus is involved in inflammatory response

Qi, Pengzhi; Huang, Huanqing; Guo, Baoying; Liao, Zhi; Liu, Huihui; Tang, Zurong; He, Yuehua

doi:10.1016/j.fsi.2018.10.018

Cited by 18 publications

(5 citation statements)

References 53 publications

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“…Healthy adult M. coruscus (about 8.0–10.5 cm in shell height) with an average weight of 60.4 ± 3.2 g were obtained from Donghe market in Zhoushan City, Zhejiang Province, China. The domestication conditions were consistent with previous studies [ 59 ]. Briefly, animals were kept in tanks with artificial sterile seawater (ASW) at 25 ± 1 °C, salinity 25%, and fed with spirulina powder.…”

Section: Methodssupporting

confidence: 91%

An Interleukin-17 Isoform from Thick Shell Mussel Mytilus coruscus Serves as a Mediator of Inflammatory Response

et al. 2023

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The inflammatory cytokine interleukin-17 (IL17) plays an important role in innate immunity by binding to its receptors (IL17Rs) to activate immune defense signals. To date, information on members of the IL17 family is still very limited in molluscan species. Here, a novel member of the IL17 family was identified and characterized from thick shell mussel Mytilus coruscus, and this gene was designated as McIL17-1 by predicting structural domains and phylogenetic analysis. McIL17-1 transcripts existed in all examined tissues with high expression levels in gills, hemocytes and digestive glands. After the stimuli of different pathogen associated molecular patterns (PAMPs) for 72 h, transcriptional expression of McIL17-1 was significantly upregulated, except for poly I:C stimulation. Cytoplasm localization of McIL17-1 was shown in HEK293T cells by fluorescence microscopy. Further, in vivo and in vitro assays were performed to evaluate the potential function of McIL17-1 played in immune response. McIL17-1 was either knocked down or overexpressed in vivo through RNA inference (RNAi) and recombinant protein injection, respectively. With the infection of living Vibrio alginolyticus, a high mortality rate was exhibited in the McIL17-1 overexpressed group compared to the control group, while a lower mortality rate was observed in the McIL17-1 knocked down group than control group. In vitro, the flow cytometric analysis showed that the apoptosis rate of McIL17-1 inhibited hemocytes was significantly lower than that of the control group after lipopolysaccharide stimulation. These results collectively suggested that the newly identified IL17 isoform is involved in the inflammatory response to bacterial infection in M. coruscus.

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Section: Methodssupporting

confidence: 91%

An Interleukin-17 Isoform from Thick Shell Mussel Mytilus coruscus Serves as a Mediator of Inflammatory Response

et al. 2023

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“…Mussels are also rich in polysaccharides that hold biological activities. Previous research showed that mussels have properties of immune regulation 19 , 20 , antioxidants 21 , preventing non-alcoholic fatty liver 22 , and reducing cholesterol levels in mice 23 . In view of the positive effects of MP on immunity, inflammatory response, oxidative stress, and other aspects, it can be hypothesized that MP may help alleviate mice in an immunosuppressive state and with poor intestinal health.…”

Section: Introductionmentioning

confidence: 99%

Immunomodulatory activity of a water-soluble polysaccharide extracted from mussel on cyclophosphamide-induced immunosuppressive mice models

Xiang

Wang

Chen³

et al. 2022

npj Sci Food

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This study aimed to investigate the protective effect of mussel polysaccharide (MP) on cyclophosphamide (Cy)-induced intestinal mucosal immunosuppression and microbial dysbiosis in mice. MP was shown to stimulate secretion of cytokines (SIgA, IL-2, IF-γ, IL-4, IL-10) and production of transcription factors (occludin, claudin-1, ZO-1, mucin-2, IL-2, IF-γ, IL-4, IL-10). Key proteins (p-IκB-α, p-p65) of the NF-κB pathway were upregulated after MP administration. SCFAs levels, which were decreased after the Cy treatment, were improved after treatment with MP. Furthermore, 16 S rRNA sequencing data of fecal samples revealed, through α-diversity and β-diversity analysis, that MP improved microbial community diversity and modulate the overall composition of gut microbiota. Taxonomic composition analysis showed that MP increased the abundance of probiotics species (Lactobacillus) and decreased the proportion of pathogenic species (Desulfovibrio). These findings suggested that MP has a potential immunomodulatory activity on the immunosuppressive mice.

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“…IRAKs have been identified in many aquatic organisms; for example, IRAK1 homologs have been identified in red tailed shrimp ( Fenneropenaeus penicillatus ) ( 77 ) and Pacific white shrimp ( 78 ), and IRAK4 homologs have been identified in an abalone ( Haliotis discus ) (GenBank accession No. KU351646.1), a thick shell mussel ( Mytilus coruscus ) ( 79 ), and a sea cucumber ( A. japonicus ) ( 39 ). Dual luciferase reporter assay data showed that 3’ UTRs of both IRAK1 and IRAK4 in aquatic animals have multiple miRNA target binding sites ( Figures 2G, H ).…”

Section: Mirnas Targeting Nf-κb Pathwaysmentioning

confidence: 99%

Non-coding RNAs targeting NF-κB pathways in aquatic animals: A review

Zhao

Zou²,

Yan³

et al. 2023

Front. Immunol.

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Nuclear factor-kappa B (NF-κB) pathways have a close relationship with many diseases, especially in terms of the regulation of inflammation and the immune response. Non-coding RNAs (ncRNAs) are a heterogeneous subset of endogenous RNAs that directly affect cellular function in the absence of proteins or peptide products; these include microRNAs (miRNAs), long noncoding RNAs (lncRNAs), circular RNAs (circRNAs), etc. Studies on the roles of ncRNAs in targeting the NF-κB pathways in aquatic animals are scarce. A few research studies have confirmed detailed regulatory mechanisms among ncRNAs and the NF-κB pathways in aquatic animals. This comprehensive review is presented concerning ncRNAs targeting the NF-κB pathway in aquatic animals and provides new insights into NF-κB pathways regulatory mechanisms of aquatic animals. The review discusses new possibilities for developing non-coding-RNA-based antiviral applications in fisheries.

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A novel interleukin-1 receptor-associated kinase-4 from thick shell mussel Mytilus coruscus is involved in inflammatory response

Cited by 18 publications

References 53 publications

An Interleukin-17 Isoform from Thick Shell Mussel Mytilus coruscus Serves as a Mediator of Inflammatory Response

An Interleukin-17 Isoform from Thick Shell Mussel Mytilus coruscus Serves as a Mediator of Inflammatory Response

Immunomodulatory activity of a water-soluble polysaccharide extracted from mussel on cyclophosphamide-induced immunosuppressive mice models

Non-coding RNAs targeting NF-κB pathways in aquatic animals: A review

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