A novel labeled and label-free dual electrochemical detection of endotoxin based on aptamer-conjugated magnetic reduced graphene oxide-gold nanocomposite

Lipopolysaccharides (LPS) are the major constituent on the cell envelope of all gram-negative bacteria. They are ubiquitous in water, air, and food, and are toxic inflammatory stimulators and optimal biomarkers for some urinary disorders and sepsis. A good many optical, electrical, thermal, and electrochemical sensors based on the intermolecular interplay of LPS with proteins, peptides, and aptamers are reported, they are generally complicated methods. We demonstrate the single-molecule nanopore approach for the identification of LPS from distinct bacteria as well as the discrimination of serotypes from the same bacterium. With a 4 nm nanopore, we achieve a LOD of 10 ng/mL and a linear response up to 10,000 ng/mL. Both the antibiotic PMB and DNA aptamer display good binding affinity to LPS, and the complexes exhibit distinct nanopore translocation behaviors compared with the individual LPS. The real sample tests in both human serum and tap water show good performance characteristics in the identification of LPS in complicated media with a nanopore platforms. Our work shows a highly-sensitive and easy-to-handle scheme for clinical and environmental biomarkers determination and provides a promising screening tool for early warning of contamination in food, water, and medical supplies.

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“…1. DNA aptamer 39,41 was melted at 95 ℃ for 5 min. before cooling down to room temperature and was left at r.t. for 30 min.…”

Section: Translocation Measurements Of Lps and Lps Complexes With Nan...mentioning

confidence: 99%

Single-Molecule Discrimination and the Specific Binding Properties of the Lipopolysaccharides with a Solid-State Nanopore sensor

Zhu

Liang

Zheng

et al. 2022

Preprint

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“…Metal oxide nanomaterials usually have higher catalytic activity than single-component nanomaterials (like gold nanoparticles), which is very useful for homogeneous dispersion of noble metal nanoparticles on a specific surface [57,58]. The synergetic effect occurs at the border of metal and oxide support which results in high catalytic activity [46,59].…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Au/Fe₃O₄/RGO based aptasensor for measurement of miRNA‐128, a biomarker for acute lymphoblastic leukemia (ALL)

Dinani

Pourmadadi

Yazdian

et al. 2022

Engineering in Life Sciences

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Due to their high sensitivity, simplicity, portability, self‐contained, and low cost, the development of electrochemical biosensors is a beneficial way to diagnose and anticipate many types of cancers. An electrochemical nanocomposite‐based aptasensor is fabricated for the determination of miRNA‐128 concentration as the acute lymphoblastic leukemia (ALL) biomarker for the first time. The aptamer chains were immobilized on the surface of the glassy carbon electrode (GCE) through gold nanoparticles/magnetite/reduced graphene oxide (AuNPs/Fe3O4/RGO). Fast Fourier transform infrared (FTIR), X‐ray diffraction (XRD), vibrating sample magnetometer (VSM), and transmission electron microscopy (TEM) were used to characterize synthesized nanomaterials. Cyclic voltammetry (CV), square wave voltammetry (SWV), and electrochemical impedance spectroscopy (EIS) were used to characterize the modified GCE in both label‐free and labeled methods. The results indicate that the modified working electrode has high selectivity and for miRNA‐128 over other biomolecules. The hexacyanoferrate redox system typically operated at around 0.3 V (vs. Ag/AgCl), and the methylene blue redox system ran at about 0 V, were used as an electrochemical probe. The detection limit and linear detection range for hexacyanoferrate and methylene blue are 0.05346 fM, 0.1–0.9 fM, and 0.005483 fM, 0.01–0.09 fM, respectively. The stability and diffusion control analyses were performed as well. In both label‐free and labeled methods, the modified electron showed high selectivity for miRNA‐128. The use of methylene blue as a safer redox mediator caused miRNA‐128 to be detected with greater accuracy at low potentials in PBS media. The findings also show the substantial improvement in detection limit and linearity by using reduced graphene oxide‐magnetite‐gold nanoparticles that can be verified by comparing with previous studies on the detection of other miRNAs.

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“…These devices are even more sensitive in detecting endotoxins; in the case of aptasensor by Zamani et al the detection limit is approximately 0.2 fg/mL. 13 A common element of all biosensors is the use of metal NPs, which enables the extremely sensitive detection of endotoxins. [13][14][15] This work describes a new method for the detection of 3-hydroxyacids that are markers of bacterial lipopolysaccharides (endotoxin).…”

Section: Introductionmentioning

confidence: 99%

“…13 A common element of all biosensors is the use of metal NPs, which enables the extremely sensitive detection of endotoxins. [13][14][15] This work describes a new method for the detection of 3-hydroxyacids that are markers of bacterial lipopolysaccharides (endotoxin). In this publication we also present the quantification result of 3-hydroxycarboxylic acids with a chemically pure silver-109 NPs produced by 1064 nm pulsed fiber laser with 2D galvanometer scanner.…”

Section: Introductionmentioning

confidence: 99%

“…Using biosensors is a novel approach for quantification and detection of endotoxin. These devices are even more sensitive in detecting endotoxins; in the case of aptasensor by Zamani et al the detection limit is approximately 0.2 fg/mL 13 . A common element of all biosensors is the use of metal NPs, which enables the extremely sensitive detection of endotoxins 13–15 …”

Section: Introductionmentioning

confidence: 99%

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Infrared pulsed fiber laser‐produced silver‐109 nanoparticles for laser desorption/ionization mass spectrometry of 3‐hydroxycarboxylic acids

Kołodziej

Płaza‐Altamer

Nizioł

et al. 2022

Rapid Comm Mass Spectrometry

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Rationale3‐Hydroxycarboxylic acids are one of the major components of bacterial lipopolysaccharides (LPS), also known as endotoxins. Endotoxins pose a serious health risk and can seriously damage the internal organs of humans and animals. 3‐Hydroxycarboxylic acids can be used as environmental markers to determine endotoxin levels. At the time of preparation of this manuscript no studies on laser mass spectrometry (MS) and analysis with silver nanoparticles (NP) for 3‐hydroxycarboxylic acids have been published in literature.MethodsSix acids, 3‐hydroxyoctanoic (3‐OH‐C8:0), 3‐hydroxydecanoic (3‐OH‐C10:0), 3‐hydroxydodecanoic (3‐OH‐C12:0), 3‐hydroxytetradecanoic (3‐OH‐C14:0), 3‐hydroxyhexadecanoic (3‐OH‐C16:0), and 3‐hydroxyoctadecanoic (3‐OH‐C18:0) acids, were used as test compounds on the target containing silver‐109 NPs for quantification using matrix‐assisted laser desorption/ionization (MALDI)–type mass spectrometer. Methods were also tested on spiked human blood serum samples to quantify 3‐hydroxycarboxylic acids and verify the influence of the biological matrix on the measurement.ResultsAnalyzed acids were directly tested in 1 000 000‐fold concentration change conditions ranging from 1 mg/mL to 1 ng/mL. The semi‐automatic MSI (MS imaging) method allowed us to obtain two to five times lower limit of detection (LOD) and lower limit of quantitation (LLOQ) values than common LDI (Bruker Daltonics, Bremen, Germany) method for analyzed acids. For almost all results of 3‐hydroxycarboxylic acids, the trendline fit was better for the semi‐automatic MSI method than the manual LDI method.ConclusionFor the first time, the use of laser MS for the quantification of 3‐hydroxycarboxylic acids has been demonstrated, and it has been proven that it can be used in the quantitative analysis of such compounds over a wide range of concentrations. In addition, a comparison of two methods—manual LDI‐MS and semi‐automatic MSI—is presented.

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A novel labeled and label-free dual electrochemical detection of endotoxin based on aptamer-conjugated magnetic reduced graphene oxide-gold nanocomposite

Cited by 36 publications

References 66 publications

Single-Molecule Discrimination and the Specific Binding Properties of the Lipopolysaccharides with a Solid-State Nanopore sensor

Single-Molecule Discrimination and the Specific Binding Properties of the Lipopolysaccharides with a Solid-State Nanopore sensor

Fabrication of Au/Fe₃O₄/RGO based aptasensor for measurement of miRNA‐128, a biomarker for acute lymphoblastic leukemia (ALL)

Infrared pulsed fiber laser‐produced silver‐109 nanoparticles for laser desorption/ionization mass spectrometry of 3‐hydroxycarboxylic acids

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A novel labeled and label-free dual electrochemical detection of endotoxin based on aptamer-conjugated magnetic reduced graphene oxide-gold nanocomposite

Cited by 36 publications

References 66 publications

Single-Molecule Discrimination and the Specific Binding Properties of the Lipopolysaccharides with a Solid-State Nanopore sensor

Single-Molecule Discrimination and the Specific Binding Properties of the Lipopolysaccharides with a Solid-State Nanopore sensor

Fabrication of Au/Fe3O4/RGO based aptasensor for measurement of miRNA‐128, a biomarker for acute lymphoblastic leukemia (ALL)

Infrared pulsed fiber laser‐produced silver‐109 nanoparticles for laser desorption/ionization mass spectrometry of 3‐hydroxycarboxylic acids

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Fabrication of Au/Fe₃O₄/RGO based aptasensor for measurement of miRNA‐128, a biomarker for acute lymphoblastic leukemia (ALL)