Economical sterile surgical gloves or sterile endobag can be used instead of expensive commercial ones to retrieve the gallbladder specimen and also intraabdominal spilt stones safely without complications in laparoscopic cholecystectomy. But the evidence available on the subject is still conflicting. Study was done with the objective of comparing the duration of surgery with the use of powder-free glove bag versus without glove bag for extraction of gallbladder specimen to determine the ease and efficacy of the use of glove bag.This was a 1 year randomized controlled trial, conducted in the Department of general surgery, Dr Prabhakar Kore hospital, KLE, Belgavi between January 2017 to January 2018. The patients were divided into group A (use of powder-free glove bag for extraction of gallbladder specimen) and group B (without the use of glove bag for extraction of gallbladder specimen). The intra-op time taken for withdrawal of the specimen in both groups was measured and compared. A total of 60 people were included in the analysis, with 30 participants each in study and control group. Among the control group, the median time taken for removal specimen was 2.05 minutes; it was 4.25 minutes was a study group. The difference in the median time taken for removal specimen between the group was statistically significant (P value <0.001).The post-operative infective complications were significantly lower in the study group, as compared to controls. But the duration and the occurrence of intraoperative bile leak were higher among study group. Hence the choice procedure needs to make with caution, considering all the risks and benefits involved.