A novel lineage-specific nuclear factor regulates mb-1 gene transcription at the early stages of B cell differentiation.

Hagman, James; Travis, Adam; Grosschedl, Rudolf

doi:10.1002/j.1460-2075.1991.tb04905.x

Cited by 140 publications

(139 citation statements)

References 40 publications

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“…5a). Two of these possess exactly the same DNA sequence as the Ebf-binding site in the mb-1 promoter where Ebf1 was first identified 33 ; therefore, we named them as M1 and M2, and two sites as E1 and E2, which have the inner palindrome of the ideal consensus site for Ebf proteins 34 . Ebf3 bound with high affinity to the M1 and E2 sites but only weakly to the E1 site as shown by electrophoretic mobility shift assay (EMSA; Fig.…”

Section: Expression Of Ebf3mentioning

confidence: 99%

Ebf factors and MyoD cooperate to regulate muscle relaxation via Atp2a1

et al. 2014

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Myogenic regulatory factors such as MyoD and Myf5 lie at the core of vertebrate muscle differentiation. However, E-boxes, the cognate binding sites for these transcription factors, are not restricted to the promoters/enhancers of muscle cell-specific genes. Thus, the specificity in myogenic transcription is poorly defined. Here we describe the transcription factor Ebf3 as a new determinant of muscle cell-specific transcription. In the absence of Ebf3 the lung does not unfold at birth, resulting in respiratory failure and perinatal death. This is due to a hypercontractile diaphragm with impaired Ca 2 þ efflux-related muscle functions. Expression of the Ca 2 þ pump Serca1 (Atp2a1) is downregulated in the absence of Ebf3, and its transgenic expression rescues this phenotype. Ebf3 binds directly to the promoter of Atp2a1 and synergises with MyoD in the induction of Atp2a1. In skeletal muscle, the homologous family member Ebf1 is strongly expressed and together with MyoD induces Atp2a1. Thus, Ebf3 is a new regulator of terminal muscle differentiation in the diaphragm, and Ebf factors cooperate with MyoD in the induction of muscle-specific genes.

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Section: Expression Of Ebf3mentioning

confidence: 99%

Ebf factors and MyoD cooperate to regulate muscle relaxation via Atp2a1

et al. 2014

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“…Nuclear protein was isolated from human IL-10-expressing Raji B cells as previously described (37). CG and AT oligonucleotides representing bp Ϫ568 to Ϫ591 of the IL-10 promoter (from the transcription start site) were synthesized: CG, 5Ј-TCGACATCCTGTGACCCCGCCTGTCCTG-3Ј and 5Ј-TCGACAGGACAGGCGGGGTCACAGGATG-3Ј; and AT, 5Ј-TCGA CATCCTGTGACCCCGCCTGTACTG-3Ј and 5Ј-TCGACAGTACAGGC GGGGTCACAGGATG-3Ј (underline indicates polymorphic residue).…”

Section: Emsamentioning

confidence: 99%

“…CG and AT oligonucleotides representing bp Ϫ568 to Ϫ591 of the IL-10 promoter (from the transcription start site) were synthesized: CG, 5Ј-TCGACATCCTGTGACCCCGCCTGTCCTG-3Ј and 5Ј-TCGACAGGACAGGCGGGGTCACAGGATG-3Ј; and AT, 5Ј-TCGA CATCCTGTGACCCCGCCTGTACTG-3Ј and 5Ј-TCGACAGTACAGGC GGGGTCACAGGATG-3Ј (underline indicates polymorphic residue). Synthesis of 32 P-labeled, double-stranded probe DNA and EMSAs was performed as previously described (37,38). Labeled probe was electrophoresed on a 6% nondenaturing polyacrylamide gel to separate labeled probe from free nucleotide, single-stranded oligonucleotides, and doublestranded oligonucleotides that were incompletely extended.…”

Section: Emsamentioning

confidence: 99%

Functional Analysis of −571 IL-10 Promoter Polymorphism Reveals a Repressor Element Controlled by Sp1

Steinke

Barekzi

Hagman

et al. 2004

The Journal of Immunology

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Transcriptional dysregulation of the IL-10 gene may contribute to the development and severity of autoimmune, infectious, neoplastic, and allergic diseases. A C to A base substitution has been identified at −571 bp in the IL-10 promoter and has been linked to immune diseases. The role of this polymorphism in IL-10 promoter function was assessed using luciferase reporter constructs. The presence of an A at −571 (A allele) increases promoter activity compared with that of a promoter with a C at this position (C allele). Binding of nuclear extract proteins from IL-10-producing human cell lines to DNA sequences including this base exchange and flanking sequences was demonstrated using EMSAs. Specific binding of the transcription factors Sp1 and Sp3 was demonstrated to a region immediately upstream of the polymorphism. No differences in the binding affinity of recombinant Sp1 were observed between the two forms of the promoter. Reconstitution of Sp1 expression decreased IL-10 promoter function in an Sp1-deficient cell line, demonstrating that this element functions as a repressor. The C to A base exchange relieves the repression mediated by Sp1. Individuals carrying the A allele of the IL-10 promoter may display increased synthesis of IL-10, resulting in suppressed immune responses and a modulation of their susceptibility to autoimmune, infectious, neoplastic, or atopic disease.

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“…E2A proteins E47 and E12 are essential for B lineage development in that they are required for expression of several important B lineage-specific genes, including Recombination-activating gene-1, CD19, VpreB, 5, mb-1, and Ig (34). EBF is a homodimeric transcription factor that is expressed at all stages of mammalian B cell development, with the exception of the terminal plasma cell stage (39). B cell differentiation in EBF-deficient mice is arrested at the pro-B cell stage; early B lineage cells with rearranged D H and J H gene segments are completely absent in these mice (40).…”

Section: Discussionmentioning

confidence: 99%

Genomic Structure and Transcriptional Regulation of the Early B Cell GenechB1

Goitsuka

Mamada

Kitamura

et al. 2001

The Journal of Immunology

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The avian B cell differentiation Ag chB1 is a membrane glycoprotein relative of the mammalian B cell differentiation Ag CD72. Unlike CD72, this C-type lectin is expressed in relatively high levels on immature B cells in the bursa of Fabricius and is down-regulated on mature B cells in the periphery. An immunoreceptor tyrosine-based inhibitory motif in the chB1 cytoplasmic tail suggests a potential regulatory role in intrabursal B cell development. To gain further insight into the selective expression and function of chB1, we determined the genomic organization of chB1 and examined the mechanism of its transcriptional regulation. The 8-exon chB1 gene proved to have very similar organization to that of mouse CD72, further supporting the idea that chB1 is a CD72 relative. As for mouse CD72, the chB1 promoter region lacks a TATA box but contains a conserved initiator element. The 131-bp region (−161 to −30) proximal to the transcriptional start site, which contains a potential early B cell factor binding site, is essential for the B lineage stage-specific transcription of chB1, whereas PU.1 and B cell-specific activator protein/Pax5 have been shown to play important roles in CD72 promoter activity and cell-type specificity. This analysis suggests that differences in transcriptional regulation of these phylogenetically related genes may determine the differences in expression pattern and, therefore, the function of avian chB1 and mammalian CD72 during B cell development.

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A novel lineage-specific nuclear factor regulates mb-1 gene transcription at the early stages of B cell differentiation.

Cited by 140 publications

References 40 publications

Ebf factors and MyoD cooperate to regulate muscle relaxation via Atp2a1

Ebf factors and MyoD cooperate to regulate muscle relaxation via Atp2a1

Functional Analysis of −571 IL-10 Promoter Polymorphism Reveals a Repressor Element Controlled by Sp1

Genomic Structure and Transcriptional Regulation of the Early B Cell GenechB1

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