A Novel Lithocholic Acid Derivative Upregulates Detoxification-Related Genes in Human Induced Pluripotent Stem Cell-Derived Intestinal Organoids

Yamada, Shigeru; Masuno, Hiroyuki; Kagechika, Hiroyuki; Tanatani, Aya; Kanda, Yasunari

doi:10.1248/bpb.b22-00492

Cited by 4 publications

(2 citation statements)

References 16 publications

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“…Dcha-20, a novel LCA derivative with vitamin-D-like activity, upregulated the expression and activity of CYP3A4, an indicator of intestinal functional maturation, in a human-induced pluripotent stem-cell-derived intestinal organoid [34]. This suggests a novel strategy to enhance the regenerative capacity of the intestinal epithelium and promote epithelial injury repair [36].…”

Section: Bile Acids Intestinal Stem Cells (Iscs) and Epithelial Injur...mentioning

confidence: 91%

Bile Acids, Intestinal Barrier Dysfunction, and Related Diseases

Shi

Jin

Huang

2023

Cells

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The intestinal barrier is a precisely regulated semi-permeable physiological structure that absorbs nutrients and protects the internal environment from infiltration of pathological molecules and microorganisms. Bile acids are small molecules synthesized from cholesterol in the liver, secreted into the duodenum, and transformed to secondary or tertiary bile acids by the gut microbiota. Bile acids interact with bile acid receptors (BARs) or gut microbiota, which plays a key role in maintaining the homeostasis of the intestinal barrier. In this review, we summarize and discuss the recent studies on bile acid disorder associated with intestinal barrier dysfunction and related diseases. We focus on the roles of bile acids, BARs, and gut microbiota in triggering intestinal barrier dysfunction. Insights for the future prevention and treatment of intestinal barrier dysfunction and related diseases are provided.

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Section: Bile Acids Intestinal Stem Cells (Iscs) and Epithelial Injur...mentioning

confidence: 91%

Bile Acids, Intestinal Barrier Dysfunction, and Related Diseases

Shi

Jin

Huang

2023

Cells

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“…Recently, Yamada et al (2022) investigated the effects of a synthetic lithocholic acid derivative, developed to have the same effects as vitamin D and referred to as Dcha‐20, on gene expression in iPSC‐derived intestinal organoids. Precisely, the authors evaluated the effects of Dcha‐20 on the gene expression of metabolizing enzymes and proteins involved in drug transport and excretion, and made a comparison with those of vitamin D. Briefly, starting from 253G1 iPSCs, definitive endoderm cells were obtained by adding activin A and CHIR99021 to the culture medium.…”

Section: Ipscs and Drug‐induced Toxicitiesmentioning

confidence: 99%

iPSCs as a groundbreaking tool for the study of adverse drug reactions: A new avenue for personalized therapy

Rispoli,

Scandiuzzi Piovesan,

Decorti

et al. 2023

WIREs Mechanisms of Disease

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Induced pluripotent stem cells (iPSCs), obtained by reprogramming different somatic cell types, represent a promising tool for the study of drug toxicities, especially in the context of personalized medicine. Indeed, these cells retain the same genetic heritage of the donor, allowing the development of personalized models. In addition, they represent a useful tool for the study of adverse drug reactions (ADRs) in special populations, such as pediatric patients, which are often poorly represented in clinical trials due to ethical issues. Particularly, iPSCs can be differentiated into any tissue of the human body, following several protocols which use different stimuli to induce specific differentiation processes. Differentiated cells also maintain the genetic heritage of the donor, and therefore are suitable for personalized pharmacological studies; moreover, iPSC‐derived differentiated cells are a valuable tool for the investigation of the mechanisms underlying the physiological differentiation processes. iPSCs‐derived organoids represent another important tool for the study of ADRs. Precisely, organoids are in vitro 3D models which better represent the native organ, both from a structural and a functional point of view. Moreover, in the same way as iPSC‐derived 2D models, iPSC‐derived organoids are appropriate personalized models since they retain the genetic heritage of the donor. In comparison to other in vitro models, iPSC‐derived organoids present advantages in terms of versatility, patient‐specificity, and ethical issues. This review aims to provide an updated report of the employment of iPSCs, and 2D and 3D models derived from these, for the study of ADRs.This article is categorized under: Cancer > Stem Cells and Development

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