A novel lupene-type triterpenic glucoside from the leaves ofClerodendrum inerme

Abstract

A new triterpenic glucoside, lup-1,5,20(29)-trien-3-O-beta-D-glucopyranoside (4), along with three known phytoconstituents: n-octacosane, friedelin and beta-amyrin, has been isolated from the leaves of Clerodendrum inerme (L.) Gaertn. (Verbenaceae). Structure elucidation was carried out on the basis of chemical and physical evidence (IR, 1H-NMR, 13C-NMR, DEPT and MS spectra). The alcoholic and aqueous extracts of the leaves of C. inerme showed significant antinociceptive activity in analgaesiometer tests.

Bioassay-guided isolation and mechanistic action of anti-inflammatory agents from Clerodendrum inerme leaves

“…inerme plant is reported to contain a variety of phytochemicals (Shrivastava and Patel, 2007b;Parveen et al, 2010;Shahabuddin et al, 2013). The isolation of acacetin from C. inerme in this study is in agreement with the earlier reports by Shrivastava and Patel (2007b).…”

“…(Verbenaceae family) known also as Sam-Ma-Nga in Thai or, seaside clerodendrum in English, occurs widely in coastal mangrove forests of Thailand (Office of Mangrove Resources Conservation, 2009) and other South Asian countries and is used in traditional medicine for the treatment of skin diseases, rheumatic pain and arthritis, fever, cough, hepatitis, and other inflammatory diseases (Shrivastava and Patel, 2007a;Chethana et al, 2013). The major chemical constituents of C. inerme are flavonoids, terpenes, steroids and phenolic compounds (Shrivastava and Patel, 2007b;Parveen et al, 2010;Shahabuddin et al, 2013). C. inerme possesses a number of biological activities including antimicrobial, anti-hepatotoxic, anti-oxidant, analgesic and anti-inflammatory activities (Gopal Contents and Sengottuvelu, 2008;Gurudeeban et al, 2010;Yankanchi and Koli, 2010;Sangeetha et al, 2011;Chethana et al, 2013).…”

Folk Medicinal Uses of Verbenaceae Family Plants in Bangladesh

“…Significant antinociceptive activity reported for alcoholic and aqueous extracts of leaves (Parveen et al, 2010). Hepatoprotective activity demonstrated by ethanolic extract of leaves against carbon tetrachloride-induced liver damage in Swiss albino rats (Gopal and Sengottuvelu, 2008).…”

“…Clerodendrum inerme has been reported to have significant antinociceptive activity (Parveen et al, 2010), which might explain its use for rheumatoid arthritis, which has quite intensive pain as one of its symptoms. Antiinflammatory properties has also been reported for Clerodendrum trichotomum (Kim et al, 2009), which again can justify its folk medicinal use in Bangladesh for treatment of rheumatoid arthritis.…”

Triterpenoids

“…Acankoreosides M, N and O, with the new genins 397-399, are also from Acanthopanax koreanum. 171 Leaves of Oplopanax horridus are the source of two saponins, one of which has a new 30-norlupane genin 410. 170 Schefflerins A-F 403-408 from Schefflera arboricola all have new genins.…”