A novel lysozyme from Xanthomonas oryzae phage ϕXo411 active against Xanthomonas and Stenotrophomonas

Lee, Chia-Ni; Lin, Juey‐Wen; Chow, Te-Yuan; Tseng, Yi-Hsiung; Weng, Shu-Fen

doi:10.1016/j.pep.2006.06.013

Cited by 29 publications

(26 citation statements)

References 30 publications

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“…The search for the presence of SAR domains revealed 103 endolysins presenting an N-terminal transmembrane domain, 38 of which have a high Gly/Ala content (40% to 60%) and fewer than three basic residues (42,96). These endolysins were annotated as presenting a putative SAR sequence and are listed in Fig.…”

Section: Resultsmentioning

confidence: 99%

Molecular Aspects and Comparative Genomics of Bacteriophage Endolysins

Oliveira

Melo

Santos

et al. 2013

J Virol

251

247

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Phages are recognized as the most abundant and diverse entities on the planet. Their diversity is determined predominantly by their dynamic adaptation capacities when confronted with different selective pressures in an endless cycle of coevolution with a widespread group of bacterial hosts. At the end of the infection cycle, progeny virions are confronted with a rigid cell wall that hinders their release into the environment and the opportunity to start a new infection cycle. Consequently, phages encode hydrolytic enzymes, called endolysins, to digest the peptidoglycan. In this work, we bring to light all phage endolysins found in completely sequenced double-stranded nucleic acid phage genomes and uncover clues that explain the phage-endolysin-host ecology that led phages to recruit unique and specialized endolysins.

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Section: Resultsmentioning

confidence: 99%

Molecular Aspects and Comparative Genomics of Bacteriophage Endolysins

Oliveira

Melo

Santos

et al. 2013

J Virol

251

247

View full text Add to dashboard Cite

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“…The supernatants were passed through a membrane filter (0.45-m pore size) and then centrifuged (15,000 ϫ g, 2 h, 4°C). The phage pellets were suspended in 1.0 ml of SM buffer (50 mM Tris-HCl [pH 7.5] containing 100 mM NaCl, 10 mM MgSO 4 , and 0.01% gelatin) and loaded on a block gradient of CsCl (1.2, 1.35, 1.45, 1.50, and 1.70 g/ml), followed by ultracentrifugation at 28,000 rpm for 2 h at 4°C with a TH641 rotor (Sorvall OTD Combi) (28). Banded phage particles were recovered and dialyzed against SM buffer.…”

Section: Methodsmentioning

confidence: 99%

Lytic Myophage Abp53 Encodes Several Proteins Similar to Those Encoded by Host Acinetobacter baumannii and Phage phiKO2

Lee

Tseng

Lin

et al. 2011

Appl Environ Microbiol

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Acinetobacter baumannii is an important Gram-negative opportunistic pathogen causing nosocomial infections. The emergence of multiple-drug-resistant A. baumannii isolates has increased in recent years. Directed toward phage therapy, a lytic phage of A. baumannii, designated Abp53, was isolated from a sputum sample in this study. Abp53 has an isometric head and a contractile tail with tail fibers (belonging to Myoviridae), a latent period of about 10 min, and a burst size of approximately 150 PFU per infected cell. Abp53 could completely lyse 27% of the A. baumannii isolates tested, which were all multiple drug resistant, but not other bacteria. Mg 2؉ enhanced the adsorption and productivity of, and host lysis by, Abp53. Twenty Abp53 virion proteins were visualized in SDS-polyacrylamide gel electrophoresis, with a 47-kDa protein being the predicted major capsid protein. Abp53 has a double-stranded DNA genome of 95 kb. Sequence analyses of a 10-kb region revealed 8 open reading frames. Five of the encoded proteins, including 3 tail components and 2 hypothetical proteins, were similar to proteins encoded by A. baumannii strain ACICU. ORF1176 (one of the tail components, 1,176 amino acids [aa]), which is also similar to tail protein gp21 of Klebsiella phage phiKO2, contained repeated domains similar to those within the ACICU_02717 protein of A. baumannii ACICU and gp21. These findings suggest a common ancestry and horizontal gene transfer during evolution. As phages can expand the host range by domain duplication in tail fiber proteins, repeated domains in ORF1176 might have a similar significance in Abp53.

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“…We found that phiL7 p28 was of the same size as and was 77% identical to the experimentally identified Xop411 lysozyme (25). P27 was predicted to be a holin, small in size (8 kDa) and similar to class III holins in having one transmembrane domain (aa 24 to 46) and a predicted periplasmic C terminus; the gene was located beside the lysozyme gene (37).…”

Section: Resultsmentioning

confidence: 54%

“…Xanthomonas oryzae pv. oryzae strain 21 and its lytic phage Xop411 have been described previously (24,25). Strains of Xanthomonas and Escherichia coli were grown at 28°C and 37°C, respectively, in TYG (tryptone, 10 g; yeast extract, 6 g; 1 ml of 1 M MgSO 4 ; 25 ml of 20% [wt/vol] glucose in deionized H 2 O to a final volume of 1 liter).…”

Section: Methodsmentioning

confidence: 99%

“…Strains of Xanthomonas and Escherichia coli were grown at 28°C and 37°C, respectively, in TYG (tryptone, 10 g; yeast extract, 6 g; 1 ml of 1 M MgSO 4 ; 25 ml of 20% [wt/vol] glucose in deionized H 2 O to a final volume of 1 liter). Phage propagation, plaque assay, purification of phage particles, isolation of phage DNA, and restriction digestion were performed as described previously (24,25).…”

Section: Methodsmentioning

confidence: 99%

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Genomic Characterization of the Intron-Containing T7-Like Phage phiL7 ofXanthomonas campestris

Lee

Lin

Weng

et al. 2009

Appl Environ Microbiol

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The lytic phage phiL7, which morphologically belongs to the Siphoviridae family, infects Xanthomonas campestris pv. campestris. Nucleotide sequence analysis has revealed that phiL7 contains a linear doublestranded DNA genome (44,080 bp, 56% G؉C) with a 3-protruding cos site (5-TTACCGGAC-3) and 59 possible genes. Among the deduced proteins, 32 have homologs with known functions and 18 show no database similarities; moreover, the genes encoding these 18 proteins mostly have varying G؉C contents and form clusters dispersed along the genome. Only 39 genes have sequences related (27% to 78%) to those of sequenced genes of X. oryzae pv. oryzae phages, although the genome size and architecture of these Xanthomonas phages are similar. These findings suggest that phiL7 acquired genes by horizontal transfer, followed by evolution via various types of mutations. Major differences were found between phiL7 and the X. oryzae pv. oryzae phages: (i) phiL7 has a group I intron inserted in the DNA polymerase gene, the first such intron observed in Xanthomonas phages; (ii) although infection of phiL7 exerted inhibition to the host RNA polymerase, similar to the situations in X. oryzae pv. oryzae phages Xp10 and Xop411, sequence analysis did not identify a homologue of the Xp10 p7 that controls the shift from host RNA polymerase (RNAP) to viral RNAP during transcription; and (iii) phiL7 lacks the tail fiber protein gene that exhibits domain duplications thought to be important for host range determination in OP1, and sequence analysis suggested that p20 (tail protein III) instead has the potential to play this role.The genus Xanthomonas in the gamma subdivision of the Proteobacteria is a diverse and economically important group of bacterial plant pathogens consisting of 27 species (35), causing tremendous loss in agriculture worldwide. Individual species of this genus comprise multiple pathogenic variants (pathovars) and collectively cause diseases on at least 124 monocot species and 268 dicot species: for example, Xanthomonas campestris pv. campestris causes black rot in crucifers, X. campestris pv. vesicatoria causes spot disease in peppers and tomatoes, X. axonopodis pv. citri causes citrus canker, and X. oryzae pv. oryzae causes bacterial leaf blight on rice plants (17). In addition, members of the genus Xanthomonas commonly produce great amounts of an exopolysaccharide (xanthan gum) and X. campestris pv. campestris has been the organism of choice in industry for production of xanthan, which is useful in food, agriculture, industry and cosmetics (55).For controlling bacterial plant diseases, chemical control with antibiotics and copper compounds has been the standard (31, 46). However, many agrochemicals are toxic and may pose significant environmental and health risks (14). Therefore, there has been a resurgence of interest recently in using bacteriophages to replace the currently prevalent chemical measures, in which phages can be used effectively as part of integrated disease management strategies (12,15,16,32). Although pha...

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A novel lysozyme from Xanthomonas oryzae phage ϕXo411 active against Xanthomonas and Stenotrophomonas

Cited by 29 publications

References 30 publications

Molecular Aspects and Comparative Genomics of Bacteriophage Endolysins

Molecular Aspects and Comparative Genomics of Bacteriophage Endolysins

Lytic Myophage Abp53 Encodes Several Proteins Similar to Those Encoded by Host Acinetobacter baumannii and Phage phiKO2

Genomic Characterization of the Intron-Containing T7-Like Phage phiL7 ofXanthomonas campestris

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